Restriction map for
pF1KSDA0908
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvaII AviII
AvrII BamHI BbrPI BbsI BbvCI
BciVI BfrI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmFI BspDI
BspEI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MluI MroI
NheI NotI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SwaI Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 886 GTCTAC GT^[AC][GT]AC
AclI 299 AACGTT AA^CGTT
AcyI 79 GGCGCC G[GA]^CG[CT]C
AspI 925 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 73 CCCGAG C^[CT]CG[GA]G
BanI 79 GGCGCC G^G[CT][GA]CC
BanII 19 GAGCTC G[GA]GC[CT]^C
BbeI 79 GGCGCC GGCGC^C
BcgI 84 CGAGCTGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1476 TGATCA T^GATCA
BcnI 1323 CCCGG CC^[GC]GG
BglI 71 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1217 AGATCT A^GATCT
BsaHI 79 GGCGCC G[GA]^CG[CT]C
BsaI 319 GGTCTC GGTCTC(1/5)
BsmBI 24 CGTCTC CGTCTC(1/5)
BsmI 578 GAATGC GAATGC(1/-1)
BsoBI 73 CCCGAG C^[CT]CG[GA]G
Bsp1407I 824 TGTACA T^GTACA
BspLU11I 458 ACATGT A^CATGT
BspMI 155 ACCTGC ACCTGC(4/8)
BsrDI 1413 CATTGC GCAATG(2/0)
BsrFI 616 GCCGGC [GA]^CCGG[CT]
BsrGI 824 TGTACA T^GTACA
Cfr10I 616 GCCGGC [GA]^CCGG[CT]
Cfr13I 216 GGGCC G^G[ACGT]CC
EarI 205 GAAGAG CTCTTC(1/4)
EcoRI 1003 GAATTC G^AATTC
FbaI 1476 TGATCA T^GATCA
HgaI 8 GCGTC GACGC(5/10)
Hin1I 79 GGCGCC G[GA]^CG[CT]C
HphI 1446 TCACC GGTGA(8/7)
KasI 79 GGCGCC G^GCGCC
Ksp632I 205 GAAGAG CTCTTC(1/4)
MfeI 995 CAATTG C^AATTG
MunI 995 CAATTG C^AATTG
NaeI 616 GCCGGC GCC^GGC
NarI 79 GGCGCC GG^CGCC
NciI 1323 CCCGG CC^[GC]GG
NdeI 1205 CATATG CA^TATG
NgoMIV 616 GCCGGC G^CCGGC
NruI 110 TCGCGA TCG^CGA
NspI 458 ACATGT [GA]CATG^[CT]
PflFI 925 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 313 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 299 AACGTT AA^CGTT
PstI 784 CTGCAG CTGCA^G
PvuII 60 CAGCTG CAG^CTG
SacI 19 GAGCTC GAGCT^C
Sau96I 216 GGGCC G^G[ACGT]CC
SfcI 784 CTGCAG C^T[GA][CT]AG
SfoI 79 GGCGCC GGC^GCC
SspBI 824 TGTACA T^GTACA
StuI 335 AGGCCT AGG^CCT
Tth111I 925 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1075 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 825 GTAC GT^AC
852 GTAC
AflIII 458 ACATGT A^C[GA][CT]GT
473 ACGTGT
AspHI 19 GAGCTC G[AT]GC[AT]^C
92 GTGCTC
BalI 675 TGGCCA TGG^CCA
938 TGGCCA
BfaI 556 CTAG C^TAG
1076 CTAG
BsiHKAI 19 GAGCTC G[AT]GC[AT]^C
92 GTGCTC
BsiYI 678 CCAAAGCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
698 CCTTTGTGGGG
BslI 678 CCAAAGCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
698 CCTTTGTGGGG
BspHI 488 TCATGA T^CATGA
513 TCATGA
BstAPI 1387 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1432 GCACTCCTTGC
BstNI 222 CCTGG CC^[AT]GG
315 CCTGG
DraI 282 TTTAAA TTT^AAA
1254 TTTAAA
DsaI 1294 CCATGG C^C[GA][CT]GG
1397 CCATGG
EaeI 675 TGGCCA [CT]^GGCC[GA]
938 TGGCCA
EcoRII 222 CCTGG ^CC[AT]GG
315 CCTGG
HaeII 79 GGCGCC [GA]GCGC^[CT]
428 GGCGCT
MaeI 556 CTAG C^TAG
1076 CTAG
MaeIII 714 GTCAC ^GT[ACGT]AC
1030 GTGAC
MluNI 675 TGGCCA TGG^CCA
938 TGGCCA
MscI 675 TGGCCA TGG^CCA
938 TGGCCA
MspA1I 60 CAGCTG C[AC]G^C[GT]G
1449 CCGCTG
MvaI 222 CCTGG CC^[AT]GG
315 CCTGG
NcoI 1294 CCATGG C^CATGG
1397 CCATGG
PleI 372 GACTC GAGTC(4/5)
906 GACTC
RcaI 488 TCATGA T^CATGA
513 TCATGA
RsaI 825 GTAC GT^AC
852 GTAC
SfaNI 68 GATGC GCATC(5/9)
790 GCATC
SspI 967 AATATT AAT^ATT
1013 AATATT
TfiI 730 GATTC G^A[AT]TC
951 GAATC
Tsp45I 714 GTCAC ^GT[GC]AC
1030 GTGAC
TspRI 716 CACTG CA[GC]TG[ACGT][ACGT]^
1116 CACTG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI BbvI BmyI BsaJI BseRI
BsgI BsmAI Bsp1286I BsrI BssKI
BstF5I BstUI BstYI Cac8I CfoI
DdeI DpnII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeII MboI MboII
MflI MnlI MseI MslI MspI
MvnI MwoI NdeII NlaIII NlaIV
Sau3AI ScrFI StyI TaqI Tru9I
TseI Tsp509I TthHB8I XhoII