Restriction map for
pF1KSDA0920
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AviII AvrII BamHI BbeI BbrPI
BcgI BfrI BglI BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoT22I FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MslI MunI NarI NdeI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 2308 GGCGTC G[GA]^CG[CT]C
AhdI 2119 GACGAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2572 CCTCAGG CC^T[ACGT]AGG
Asp700I 369 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2119 GACGAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 2142 GGATAC GTATCC(6/5)
BglII 2071 AGATCT A^GATCT
BlpI 2755 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2755 GCTGAGC GC^T[ACGT]AGC
BsaHI 2308 GGCGTC G[GA]^CG[CT]C
BsmBI 2310 CGTCTC CGTCTC(1/5)
BspHI 1599 TCATGA T^CATGA
BsrBI 488 GAGCGG CCGCTC(-3/-3)
BsrDI 1401 CATTGC GCAATG(2/0)
BsrFI 1016 GCCGGC [GA]^CCGG[CT]
BssSI 976 CACGAG CACGAG(-5/-1)
Bsu36I 2572 CCTCAGG CC^T[ACGT]AGG
CelII 2755 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1016 GCCGGC [GA]^CCGG[CT]
Eam1105I 2119 GACGAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2572 CCTCAGG CC^T[ACGT]AGG
EcoRI 1695 GAATTC G^AATTC
HgaI 2309 GCGTC GACGC(5/10)
Hin1I 2308 GGCGTC G[GA]^CG[CT]C
HincII 1777 GTCAAC GT[CT]^[GA]AC
HindII 1777 GTCAAC GT[CT]^[GA]AC
NaeI 1016 GCCGGC GCC^GGC
NgoMIV 1016 GCCGGC G^CCGGC
NheI 1012 GCTAGC G^CTAGC
PaeR7I 3217 CTCGAG C^TCGAG
PshAI 2107 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1599 TCATGA T^CATGA
SbfI 1131 CCTGCAGG CCTGCA^GG
ScaI 2741 AGTACT AGT^ACT
SmaI 2012 CCCGGG CCC^GGG
Sse8387I 1131 CCTGCAGG CCTGCA^GG
XbaI 858 TCTAGA T^CTAGA
XcmI 1700 CCAGGCAAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3217 CTCGAG C^TCGAG
XmaCI 2012 CCCGGG C^CCGGG
XmaI 2012 CCCGGG C^CCGGG
XmnI 369 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 1606 CGCG CG^CG
3151 CGCG
AspHI 1039 GAGCTC G[AT]GC[AT]^C
1363 GAGCTC
AspI 1636 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2844 GACTTTGTC
BclI 810 TGATCA T^GATCA
2328 TGATCA
BsiHKAI 1039 GAGCTC G[AT]GC[AT]^C
1363 GAGCTC
BstUI 1606 CGCG CG^CG
3151 CGCG
BstXI 644 CCACCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2147 CCACAGTCCTGG
EcoRV 300 GATATC GAT^ATC
607 GATATC
FbaI 810 TGATCA T^GATCA
2328 TGATCA
HaeII 2008 AGCGCC [GA]GCGC^[CT]
2640 GGCGCT
MseI 2699 TTAA T^TAA
3233 TTAA
MvnI 1606 CGCG CG^CG
3151 CGCG
NspI 2558 GCATGT [GA]CATG^[CT]
2974 ACATGC
PflFI 1636 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2844 GACTTTGTC
PflMI 460 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
928 CCAGAGCCTGG
SacI 1039 GAGCTC GAGCT^C
1363 GAGCTC
StuI 1321 AGGCCT AGG^CCT
1762 AGGCCT
Tru9I 2699 TTAA T^TAA
3233 TTAA
Tth111I 1636 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2844 GACTTTGTC
Van91I 460 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
928 CCAGAGCCTGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
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AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII