Restriction map for
pF1KSDA0921
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# NO CUT ENZYMES ( 66 enzymes) #
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AccI AccIII AclI AcsI AflII
AgeI ApoI AscI AseI AsnI
BbsI BciVI BclI BfrI BglII
BpuAI BsaBI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspLU11I Bst1107I
BstBI BstZ17I ClaI DraI EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI MamI MfeI MluI MroI
MunI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI SalI SfiI SfuI SnaBI
SpeI SphI SwaI Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 25 enzymes) #
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Acc65I 3626 GGTACC G^GTACC
AhdI 3786 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2917 GTGCAC G^TGCAC
ApaLI 2917 GTGCAC G^TGCAC
Asp718I 3626 GGTACC G^GTACC
AspEI 3786 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 170 TGCGCA TGC^GCA
AvrII 981 CCTAGG C^CTAGG
BamHI 1124 GGATCC G^GATCC
BlnI 981 CCTAGG C^CTAGG
CpoI 4352 CGGACCG CG^G[AT]CCG
Eam1105I 3786 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 170 TGCGCA TGC^GCA
KpnI 3626 GGTACC GGTAC^C
NdeI 2434 CATATG CA^TATG
RsrII 4352 CGGACCG CG^G[AT]CCG
SapI 1497 GCTCTTC GCTCTTC(1/4)
SbfI 3594 CCTGCAGG CCTGCA^GG
ScaI 4036 AGTACT AGT^ACT
SexAI 4793 ACCAGGT A^CC[AT]GGT
SgrAI 314 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 3594 CCTGCAGG CCTGCA^GG
SspI 4053 AATATT AAT^ATT
TfiI 1761 GATTC G^A[AT]TC
Tsp509I 1292 AATT ^AATT
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# TWO CUT ENZYMES ( 28 enzymes) #
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AatII 1051 GACGTC GACGT^C
3098 GACGTC
AflIII 2810 ACGTGT A^C[GA][CT]GT
3329 ACACGT
AocI 1801 CCTGAGG CC^T[ACGT]AGG
4401 CCTCAGG
Asp700I 2034 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3018 GAATATGTTC
AspI 931 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1264 GACGTGGTC
BbvCI 4592 CCTCAGC CCTCAGC(-5/-2)
1927 GCTGAGG
BcgI 1560 CGACTACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
108 GCAGTGGGCTCG
BfaI 982 CTAG C^TAG
1462 CTAG
Bsp1407I 2123 TGTACA T^GTACA
2996 TGTACA
BspHI 2210 TCATGA T^CATGA
2471 TCATGA
BsrGI 2123 TGTACA T^GTACA
2996 TGTACA
BssSI 3357 CACGAG CACGAG(-5/-1)
2533 CTCGTG
BstYI 1124 GGATCC [GA]^GATC[CT]
1615 GGATCT
Bsu36I 1801 CCTGAGG CC^T[ACGT]AGG
4401 CCTCAGG
Eco81I 1801 CCTGAGG CC^T[ACGT]AGG
4401 CCTCAGG
EcoNI 1308 CCTGGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1923 CCTGGCTGAGG
KspI 525 CCGCGG CCGC^GG
4558 CCGCGG
MaeI 982 CTAG C^TAG
1462 CTAG
MflI 1124 GGATCC [GA]^GATC[CT]
1615 GGATCT
PflFI 931 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1264 GACGTGGTC
RcaI 2210 TCATGA T^CATGA
2471 TCATGA
SacI 154 GAGCTC GAGCT^C
2593 GAGCTC
SacII 525 CCGCGG CCGC^GG
4558 CCGCGG
SspBI 2123 TGTACA T^GTACA
2996 TGTACA
StuI 1409 AGGCCT AGG^CCT
4262 AGGCCT
Tth111I 931 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1264 GACGTGGTC
XhoII 1124 GGATCC [GA]^GATC[CT]
1615 GGATCT
XmnI 2034 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3018 GAATATGTTC
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# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssHII
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I XcmI XmaCI XmaI