Restriction map for
pF1KSDA0924
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII AccI AccII AccIII AcyI
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KspI MaeI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 773 GGTACC G^GTACC
AclI 793 AACGTT AA^CGTT
Alw44I 1120 GTGCAC G^TGCAC
AlwI 1515 GATCC GGATC(4/5)
ApaLI 1120 GTGCAC G^TGCAC
Asp718I 773 GGTACC G^GTACC
AspHI 1120 GTGCAC G[AT]GC[AT]^C
AvaII 935 GGTCC G^G[AT]CC
BalI 1422 TGGCCA TGG^CCA
BanII 685 GAGCCC G[GA]GC[CT]^C
BbsI 283 GAAGAC GAAGAC(2/6)
BcnI 288 CCGGG CC^[GC]GG
BpuAI 283 GAAGAC GAAGAC(2/6)
BsgI 1648 CTGCAC GTGCAG(16/14)
BsiHKAI 1120 GTGCAC G[AT]GC[AT]^C
BsiYI 1758 CCAGAGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1758 CCAGAGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 149 TGTACA T^GTACA
BspHI 942 TCATGA T^CATGA
BsrDI 497 GCAATG GCAATG(2/0)
BsrGI 149 TGTACA T^GTACA
BstAPI 1122 GCACTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1479 GGTGACC G^GT[ACGT]ACC
BstPI 1479 GGTGACC G^GT[ACGT]ACC
BstXI 1245 CCAGTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1514 AGATCC [GA]^GATC[CT]
CfoI 807 GCGC GCG^C
DraI 1743 TTTAAA TTT^AAA
EaeI 1422 TGGCCA [CT]^GGCC[GA]
EcoO65I 1479 GGTGACC G^GT[ACGT]ACC
EcoRI 294 GAATTC G^AATTC
HapII 288 CCGG C^CGG
HhaI 807 GCGC GCG^C
HinP1I 807 GCGC G^CGC
HindIII 1344 AAGCTT A^AGCTT
HpaII 288 CCGG C^CGG
KpnI 773 GGTACC GGTAC^C
MflI 1514 AGATCC [GA]^GATC[CT]
MluNI 1422 TGGCCA TGG^CCA
MscI 1422 TGGCCA TGG^CCA
MspA1I 1426 CAGCGG C[AC]G^C[GT]G
MspI 288 CCGG C^CGG
NciI 288 CCGGG CC^[GC]GG
Psp1406I 793 AACGTT AA^CGTT
RcaI 942 TCATGA T^CATGA
ScaI 1277 AGTACT AGT^ACT
SspBI 149 TGTACA T^GTACA
XhoII 1514 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AflII 439 CTTAAG C^TTAAG
1450 CTTAAG
AlwNI 25 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
257 CAGTGTCTG
Asp700I 1005 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1174 GAAAGGTTTC
BfrI 439 CTTAAG C^TTAAG
1450 CTTAAG
BseRI 310 GAGGAG GAGGAG(10/8)
454 GAGGAG
BsmI 1134 GCATTC GAATGC(1/-1)
1214 GCATTC
BspMI 1503 ACCTGC ACCTGC(4/8)
1646 ACCTGC
DsaI 963 CCATGG C^C[GA][CT]GG
1019 CCATGG
EcoT14I 963 CCATGG C^C[AT][AT]GG
1019 CCATGG
FauI 1710 CCCGC CCCGC(4/6)
672 GCGGG
MaeII 266 ACGT A^CGT
794 ACGT
NcoI 963 CCATGG C^CATGG
1019 CCATGG
PleI 429 GACTC GAGTC(4/5)
729 GACTC
SphI 270 GCATGC GCATG^C
809 GCATGC
StyI 963 CCATGG C^C[AT][AT]GG
1019 CCATGG
TaqI 1283 TCGA T^CGA
1441 TCGA
TfiI 160 GAATC G^A[AT]TC
1596 GAATC
TthHB8I 1283 TCGA T^CGA
1441 TCGA
XmnI 1005 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1174 GAAAGGTTTC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BanI BbvI BmyI BpmI BsaJI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI Tru9I TseI
Tsp45I Tsp509I TspRI