Restriction map for
pF1KSDA0936
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# NO CUT ENZYMES ( 136 enzymes) #
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Acc65I AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BanI
BbeI BbrPI BbsI BcgI BciVI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI FauI FseI FspI
HaeII HgaI HhaI HinP1I HindIII
HpaI KasI KpnI KspI MaeIII
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tsp45I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 519 GTCTAC GT^[AC][GT]AC
AlwNI 775 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1171 GAGCAC G[AT]GC[AT]^C
AspI 1590 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 712 TGGCCA TGG^CCA
BamHI 801 GGATCC G^GATCC
BanII 497 GGGCTC G[GA]GC[CT]^C
BbvCI 1287 CCTCAGC CCTCAGC(-5/-2)
BclI 216 TGATCA T^GATCA
BglII 1444 AGATCT A^GATCT
BsaAI 1041 CACGTA [CT]AC^GT[GA]
BseRI 391 CTCCTC GAGGAG(10/8)
BsiHKAI 1171 GAGCAC G[AT]GC[AT]^C
BsmI 130 GAATGC GAATGC(1/-1)
BsrDI 697 GCAATG GCAATG(2/0)
DraI 159 TTTAAA TTT^AAA
DraIII 716 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 595 CTCTTC CTCTTC(1/4)
EcoRV 839 GATATC GAT^ATC
EcoT14I 1552 CCATGG C^C[AT][AT]GG
EcoT22I 132 ATGCAT ATGCA^T
FbaI 216 TGATCA T^GATCA
HapII 1860 CCGG C^CGG
HincII 1230 GTCAAC GT[CT]^[GA]AC
HindII 1230 GTCAAC GT[CT]^[GA]AC
HpaII 1860 CCGG C^CGG
Ksp632I 595 CTCTTC CTCTTC(1/4)
MluNI 712 TGGCCA TGG^CCA
MscI 712 TGGCCA TGG^CCA
MspI 1860 CCGG C^CGG
NcoI 1552 CCATGG C^CATGG
NheI 757 GCTAGC G^CTAGC
NsiI 132 ATGCAT ATGCA^T
NspI 788 ACATGC [GA]CATG^[CT]
PflFI 1590 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1160 CAGCTG CAG^CTG
ScaI 507 AGTACT AGT^ACT
SspI 624 AATATT AAT^ATT
StyI 1552 CCATGG C^C[AT][AT]GG
Tth111I 1590 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AatII 544 GACGTC GACGT^C
1410 GACGTC
AcyI 544 GACGTC G[GA]^CG[CT]C
1410 GACGTC
AocI 513 CCTGAGG CC^T[ACGT]AGG
585 CCTCAGG
AvaI 28 CTCGGG C^[CT]CG[GA]G
440 CCCGAG
AvaII 403 GGACC G^G[AT]CC
1359 GGACC
BsaHI 544 GACGTC G[GA]^CG[CT]C
1410 GACGTC
BsaI 1767 GAGACC GGTCTC(1/5)
1062 GGTCTC
BsoBI 28 CTCGGG C^[CT]CG[GA]G
440 CCCGAG
BspMI 963 ACCTGC ACCTGC(4/8)
1340 GCAGGT
BstYI 801 GGATCC [GA]^GATC[CT]
1444 AGATCT
Bsu36I 513 CCTGAGG CC^T[ACGT]AGG
585 CCTCAGG
DsaI 1552 CCATGG C^C[GA][CT]GG
1828 CCACGG
EaeI 712 TGGCCA [CT]^GGCC[GA]
1857 TGGCCG
Eco81I 513 CCTGAGG CC^T[ACGT]AGG
585 CCTCAGG
EcoNI 141 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
921 CCTGGAAAAGG
Hin1I 544 GACGTC G[GA]^CG[CT]C
1410 GACGTC
HphI 1183 GGTGA GGTGA(8/7)
1077 TCACC
MflI 801 GGATCC [GA]^GATC[CT]
1444 AGATCT
MslI 175 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1174 CACAAAAATG
MspA1I 1160 CAGCTG C[AC]G^C[GT]G
1420 CAGCGG
PflMI 639 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1849 CCAGTGCATGG
PstI 1338 CTGCAG CTGCA^G
1448 CTGCAG
Van91I 639 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1849 CCAGTGCATGG
XcmI 1543 CCACCTAATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1845 CCAGCCAGTGCATGG
XhoII 801 GGATCC [GA]^GATC[CT]
1444 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XmnI