Restriction map for
pF1KSDA0940
##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbrPI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsiHKAI BsmBI
Bsp1407I BspDI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII ClaI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HpaI KpnI MamI
MfeI MflI MluI MluNI MscI
MslI MunI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI SacI
SalI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
StuI SwaI Tth111I XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 1363 GTAGAC GT^[AC][GT]AC
AccIII 968 TCCGGA T^CCGGA
AcyI 594 GGCGCC G[GA]^CG[CT]C
AflII 1227 CTTAAG C^TTAAG
AlwI 377 GGATC GGATC(4/5)
AocI 277 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1783 AGCGCT AGC^GCT
AvaI 1973 CCCGAG C^[CT]CG[GA]G
BanII 283 GAGCCC G[GA]GC[CT]^C
BbeI 594 GGCGCC GGCGC^C
BcgI 893 GCAACGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 94 GTATCC GTATCC(6/5)
BfrI 1227 CTTAAG C^TTAAG
BsaHI 594 GGCGCC G[GA]^CG[CT]C
BsaI 127 GAGACC GGTCTC(1/5)
BseAI 968 TCCGGA T^CCGGA
BsgI 1795 GTGCAG GTGCAG(16/14)
BsiWI 626 CGTACG C^GTACG
BsmAI 127 GAGAC GTCTC(1/5)
BsoBI 1973 CCCGAG C^[CT]CG[GA]G
BspEI 968 TCCGGA T^CCGGA
BspMI 1343 GCAGGT ACCTGC(4/8)
BstAPI 679 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 277 CCTCAGG CC^T[ACGT]AGG
CpoI 970 CGGACCG CG^G[AT]CCG
DraIII 1883 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 403 CTCTTC CTCTTC(1/4)
Eco47III 1783 AGCGCT AGC^GCT
Eco81I 277 CCTCAGG CC^T[ACGT]AGG
Hin1I 594 GGCGCC G[GA]^CG[CT]C
HindIII 1451 AAGCTT A^AGCTT
KasI 594 GGCGCC G^GCGCC
Ksp632I 403 CTCTTC CTCTTC(1/4)
MroI 968 TCCGGA T^CCGGA
NarI 594 GGCGCC GG^CGCC
NdeI 1841 CATATG CA^TATG
PleI 85 GAGTC GAGTC(4/5)
PpuMI 797 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 720 CAGCTG CAG^CTG
RsrII 970 CGGACCG CG^G[AT]CCG
SapI 402 GCTCTTC GCTCTTC(1/4)
SbfI 783 CCTGCAGG CCTGCA^GG
SexAI 328 ACCTGGT A^CC[AT]GGT
SfoI 594 GGCGCC GGC^GCC
Sse8387I 783 CCTGCAGG CCTGCA^GG
SspI 1135 AATATT AAT^ATT
TfiI 220 GAATC G^A[AT]TC
XbaI 1276 TCTAGA T^CTAGA
XcmI 941 CCAAGTCCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 1110 AAATTT [GA]^AATT[CT]
1535 AAATTC
ApoI 1110 AAATTT [GA]^AATT[CT]
1535 AAATTC
BbsI 436 GTCTTC GAAGAC(2/6)
666 GTCTTC
BbvCI 165 CCTCAGC CCTCAGC(-5/-2)
259 CCTCAGC
BfaI 1277 CTAG C^TAG
1471 CTAG
BpuAI 436 GTCTTC GAAGAC(2/6)
666 GTCTTC
BsaWI 968 TCCGGA [AT]^CCGG[AT]
1007 ACCGGA
BsiEI 812 CGGTCG CG[GA][CT]^CG
2019 CGACCG
BsmI 744 GAATGC GAATGC(1/-1)
1747 GCATTC
BspHI 644 TCATGA T^CATGA
1080 TCATGA
BsrDI 1770 CATTGC GCAATG(2/0)
1654 GCAATG
BsrFI 159 GCCGGC [GA]^CCGG[CT]
522 GCCGGC
Cfr10I 159 GCCGGC [GA]^CCGG[CT]
522 GCCGGC
DraII 797 GGGACCC [GA]G^G[ACGT]CC[CT]
1015 AGGCCCT
EaeI 630 CGGCCA [CT]^GGCC[GA]
1737 TGGCCG
EcoNI 1087 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1402 CCTCCTAAAGG
EcoO109I 797 GGGACCC [GA]G^G[ACGT]CC[CT]
1015 AGGCCCT
EcoT14I 1200 CCATGG C^C[AT][AT]GG
1543 CCATGG
HgaI 1097 GACGC GACGC(5/10)
301 GCGTC
HincII 388 GTCAAC GT[CT]^[GA]AC
427 GTCAAC
HindII 388 GTCAAC GT[CT]^[GA]AC
427 GTCAAC
HinfI 85 GAGTC G^A[ACGT]TC
220 GAATC
KspI 287 CCGCGG CCGC^GG
620 CCGCGG
MaeI 1277 CTAG C^TAG
1471 CTAG
NaeI 159 GCCGGC GCC^GGC
522 GCCGGC
NcoI 1200 CCATGG C^CATGG
1543 CCATGG
NgoMIV 159 GCCGGC G^CCGGC
522 GCCGGC
PflMI 767 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
941 CCAAGTCCTGG
PstI 784 CTGCAG CTGCA^G
1933 CTGCAG
RcaI 644 TCATGA T^CATGA
1080 TCATGA
SacII 287 CCGCGG CCGC^GG
620 CCGCGG
SfcI 784 CTGCAG C^T[GA][CT]AG
1933 CTGCAG
SmlI 240 CTTGAG C^T[CT][GA]AG
1227 CTTAAG
StyI 1200 CCATGG C^C[AT][AT]GG
1543 CCATGG
Tsp509I 1111 AATT ^AATT
1536 AATT
Van91I 767 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
941 CCAAGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BanI BbvI BclI BcnI
BglI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EcoRII FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I