Restriction map for
pF1KSDA0948
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI AvrII BamHI BciVI BclI
BfrI BglII BlnI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FauI FbaI FseI HincII
HindII HindIII HpaI Ksp632I KspI
MamI MfeI MflI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SapI SfiI SfuI
SgrAI SmaI SnaBI SpeI SspBI
SspI SwaI Tru9I Tth111I Van91I
XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 1462 GACGTC GACGT^C
Acc65I 430 GGTACC G^GTACC
AccII 800 CGCG CG^CG
AcsI 970 GAATTT [GA]^AATT[CT]
ApoI 970 GAATTT [GA]^AATT[CT]
Asp700I 1014 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 430 GGTACC G^GTACC
AspHI 250 GAGCTC G[AT]GC[AT]^C
AviII 1532 TGCGCA TGC^GCA
BbeI 1510 GGCGCC GGCGC^C
BbrPI 27 CACGTG CAC^GTG
BbsI 727 GAAGAC GAAGAC(2/6)
BcgI 22 CGATGCACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1424 CCCGG CC^[GC]GG
BglI 553 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 613 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 613 GCTGAGC GC^T[ACGT]AGC
BpuAI 727 GAAGAC GAAGAC(2/6)
BsaAI 27 CACGTG [CT]AC^GT[GA]
BsgI 777 GTGCAG GTGCAG(16/14)
BsiHKAI 250 GAGCTC G[AT]GC[AT]^C
BsmBI 1162 GAGACG CGTCTC(1/5)
BspHI 680 TCATGA T^CATGA
BsrDI 301 GCAATG GCAATG(2/0)
BsrFI 168 GCCGGT [GA]^CCGG[CT]
BstAPI 600 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1134 GGTGACC G^GT[ACGT]ACC
BstPI 1134 GGTGACC G^GT[ACGT]ACC
BstUI 800 CGCG CG^CG
BstXI 1308 CCAGCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 613 GCTGAGC GC^T[ACGT]AGC
Cfr10I 168 GCCGGT [GA]^CCGG[CT]
DraIII 1432 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 730 GACAAGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 65 CCATGG C^C[GA][CT]GG
EcoO65I 1134 GGTGACC G^GT[ACGT]ACC
FspI 1532 TGCGCA TGC^GCA
KasI 1510 GGCGCC G^GCGCC
KpnI 430 GGTACC GGTAC^C
MslI 666 CACATTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 516 CCGCTG C[AC]G^C[GT]G
MvnI 800 CGCG CG^CG
NarI 1510 GGCGCC GG^CGCC
NciI 1424 CCCGG CC^[GC]GG
NcoI 65 CCATGG C^CATGG
NheI 1350 GCTAGC G^CTAGC
NspI 524 GCATGC [GA]CATG^[CT]
PaeR7I 1204 CTCGAG C^TCGAG
PmaCI 27 CACGTG CAC^GTG
PmlI 27 CACGTG CAC^GTG
PpuMI 1077 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 680 TCATGA T^CATGA
SacI 250 GAGCTC GAGCT^C
SbfI 891 CCTGCAGG CCTGCA^GG
ScaI 1070 AGTACT AGT^ACT
SfoI 1510 GGCGCC GGC^GCC
SphI 524 GCATGC GCATG^C
Sse8387I 891 CCTGCAGG CCTGCA^GG
TfiI 658 GATTC G^A[AT]TC
XhoI 1204 CTCGAG C^TCGAG
XmnI 1014 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1462 GACGTC G[GA]^CG[CT]C
1510 GGCGCC
AlwI 828 GGATC GGATC(4/5)
1008 GGATC
AlwNI 601 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1024 CAGAAGCTG
ApaI 911 GGGCCC GGGCC^C
1111 GGGCCC
BalI 1211 TGGCCA TGG^CCA
1556 TGGCCA
BfaI 988 CTAG C^TAG
1351 CTAG
BsaHI 1462 GACGTC G[GA]^CG[CT]C
1510 GGCGCC
BseRI 247 GAGGAG GAGGAG(10/8)
1549 GAGGAG
BspMI 210 GCAGGT ACCTGC(4/8)
894 GCAGGT
DpnII 829 GATC ^GATC
1009 GATC
EcoNI 579 CCTCTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1143 CCTGCAAAAGG
HaeII 1186 AGCGCC [GA]GCGC^[CT]
1510 GGCGCC
HgaI 1164 GACGC GACGC(5/10)
801 GCGTC
Hin1I 1462 GACGTC G[GA]^CG[CT]C
1510 GGCGCC
MaeI 988 CTAG C^TAG
1351 CTAG
MboI 829 GATC ^GATC
1009 GATC
MluNI 1211 TGGCCA TGG^CCA
1556 TGGCCA
MscI 1211 TGGCCA TGG^CCA
1556 TGGCCA
NdeII 829 GATC ^GATC
1009 GATC
PleI 111 GACTC GAGTC(4/5)
565 GAGTC
Sau3AI 829 GATC ^GATC
1009 GATC
SexAI 11 ACCTGGT A^CC[AT]GGT
902 ACCTGGT
StuI 479 AGGCCT AGG^CCT
876 AGGCCT
TaqI 439 TCGA T^CGA
1205 TCGA
TspRI 1047 CACTG CA[GC]TG[ACGT][ACGT]^
1257 CAGTG
TthHB8I 439 TCGA T^CGA
1205 TCGA
XcmI 1046 CCACTGCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1468 CCATTCTATGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanI BanII BbvCI BbvI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboII MnlI
MspI MvaI MwoI NlaIII NlaIV
PstI RsaI Sau96I ScrFI SfaNI
SfcI SmlI StyI TseI Tsp45I
Tsp509I