Restriction map for
pF1KSDA0955
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# NO CUT ENZYMES ( 145 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfaI BfrI BglI BglII
BlnI BpuAI BsaAI BsaI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HindIII HpaI
KasI KpnI KspI MaeI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 466 GACGTC GACGT^C
AfaI 684 GTAC GT^AC
Asp700I 916 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 527 CCCGGG C^[CT]CG[GA]G
BanII 864 GAGCCC G[GA]GC[CT]^C
BciVI 966 GGATAC GTATCC(6/5)
BlpI 1188 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1188 GCTGAGC GC^T[ACGT]AGC
BsaBI 576 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1031 GTGCAG GTGCAG(16/14)
BsmBI 468 CGTCTC CGTCTC(1/5)
BsoBI 527 CCCGGG C^[CT]CG[GA]G
BspMI 347 ACCTGC ACCTGC(4/8)
BsrFI 1055 ACCGGC [GA]^CCGG[CT]
BssSI 1264 CTCGTG CACGAG(-5/-1)
BstYI 987 GGATCT [GA]^GATC[CT]
CelII 1188 GCTGAGC GC^T[ACGT]AGC
CfoI 753 GCGC GCG^C
Cfr10I 1055 ACCGGC [GA]^CCGG[CT]
DsaI 744 CCATGG C^C[GA][CT]GG
EcoNI 1021 CCTTTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 700 GACGC GACGC(5/10)
HhaI 753 GCGC GCG^C
HinP1I 753 GCGC G^CGC
HincII 49 GTCAAC GT[CT]^[GA]AC
HindII 49 GTCAAC GT[CT]^[GA]AC
MamI 576 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 987 GGATCT [GA]^GATC[CT]
NcoI 744 CCATGG C^CATGG
RsaI 684 GTAC GT^AC
SapI 1230 GCTCTTC GCTCTTC(1/4)
SmaI 527 CCCGGG CCC^GGG
SmlI 928 CTTGAG C^T[CT][GA]AG
TfiI 96 GAATC G^A[AT]TC
XhoII 987 GGATCT [GA]^GATC[CT]
XmaCI 527 CCCGGG C^CCGGG
XmaI 527 CCCGGG C^CCGGG
XmnI 916 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 377 GCGG CCGC(-3/-1)
591 GCGG
AcyI 466 GACGTC G[GA]^CG[CT]C
700 GACGCC
AlwI 593 GGATC GGATC(4/5)
987 GGATC
BcnI 527 CCCGG CC^[GC]GG
528 CCGGG
BsaHI 466 GACGTC G[GA]^CG[CT]C
700 GACGCC
BseRI 1016 CTCCTC GAGGAG(10/8)
409 GAGGAG
Cac8I 244 GCTGGC GC[ACGT]^[ACGT]GC
755 GCCTGC
EarI 1231 CTCTTC CTCTTC(1/4)
91 GAAGAG
HapII 528 CCGG C^CGG
1056 CCGG
Hin1I 466 GACGTC G[GA]^CG[CT]C
700 GACGCC
HpaII 528 CCGG C^CGG
1056 CCGG
Ksp632I 1231 CTCTTC CTCTTC(1/4)
91 GAAGAG
MaeII 467 ACGT A^CGT
1226 ACGT
MseI 494 TTAA T^TAA
671 TTAA
MspI 528 CCGG C^CGG
1056 CCGG
NciI 527 CCCGG CC^[GC]GG
528 CCGGG
PleI 606 GACTC GAGTC(4/5)
327 GAGTC
StuI 270 AGGCCT AGG^CCT
1181 AGGCCT
TaqI 140 TCGA T^CGA
1097 TCGA
Tru9I 494 TTAA T^TAA
671 TTAA
TthHB8I 140 TCGA T^CGA
1097 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AluI AlwNI ApoI AspHI
AvaII BbvI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV PpuMI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I Tsp509I TspRI