Restriction map for
pF1KSDA0957
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII AvrII BamHI BbrPI
BbvCI BciVI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssSI Bst1107I BstBI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT22I FseI FspI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 97 GGCGCC G[GA]^CG[CT]C
AhdI 1527 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1204 GGATC GGATC(4/5)
ApaI 1726 GGGCCC GGGCC^C
AspEI 1527 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 465 GAGCAC G[AT]GC[AT]^C
BanII 1726 GGGCCC G[GA]GC[CT]^C
BbeI 97 GGCGCC GGCGC^C
BbsI 1632 GTCTTC GAAGAC(2/6)
BcgI 288 CGAAGGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1713 TGATCA T^GATCA
BglI 1776 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1632 GTCTTC GAAGAC(2/6)
BsaHI 97 GGCGCC G[GA]^CG[CT]C
BsiEI 17 CGGTCG CG[GA][CT]^CG
BsiHKAI 465 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1642 TGTACA T^GTACA
BsrGI 1642 TGTACA T^GTACA
BssHII 544 GCGCGC G^CGCGC
BstAPI 1260 GCACCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 111 GGTTACC G^GT[ACGT]ACC
BstPI 111 GGTTACC G^GT[ACGT]ACC
DrdI 199 GACCTTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1527 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 111 GGTTACC G^GT[ACGT]ACC
EcoRV 1380 GATATC GAT^ATC
FbaI 1713 TGATCA T^GATCA
Hin1I 97 GGCGCC G[GA]^CG[CT]C
HindIII 1441 AAGCTT A^AGCTT
HpaI 706 GTTAAC GTT^AAC
KasI 97 GGCGCC G^GCGCC
MslI 246 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 727 CCGCTG C[AC]G^C[GT]G
NarI 97 GGCGCC GG^CGCC
PpuMI 490 GGGTCCC [GA]G^G[AT]CC[CT]
SapI 766 GCTCTTC GCTCTTC(1/4)
SexAI 1405 ACCTGGT A^CC[AT]GGT
SfoI 97 GGCGCC GGC^GCC
SphI 990 GCATGC GCATG^C
SspBI 1642 TGTACA T^GTACA
TaqI 1131 TCGA T^CGA
Tsp45I 1525 GTGAC ^GT[GC]AC
TthHB8I 1131 TCGA T^CGA
XcmI 668 CCAAAATCTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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BalI 665 TGGCCA TGG^CCA
836 TGGCCA
BanI 97 GGCGCC G^G[CT][GA]CC
1113 GGCACC
BfaI 1457 CTAG C^TAG
2057 CTAG
BglII 176 AGATCT A^GATCT
1708 AGATCT
BspMI 986 ACCTGC ACCTGC(4/8)
1531 GCAGGT
BstYI 176 AGATCT [GA]^GATC[CT]
1708 AGATCT
EcoT14I 923 CCAAGG C^C[AT][AT]GG
1385 CCTTGG
HgaI 473 GCGTC GACGC(5/10)
1733 GCGTC
HincII 706 GTTAAC GT[CT]^[GA]AC
1735 GTCAAC
HindII 706 GTTAAC GT[CT]^[GA]AC
1735 GTCAAC
HphI 1524 GGTGA GGTGA(8/7)
1002 TCACC
MaeI 1457 CTAG C^TAG
2057 CTAG
MaeIII 112 GTTAC ^GT[ACGT]AC
1525 GTGAC
MflI 176 AGATCT [GA]^GATC[CT]
1708 AGATCT
MluNI 665 TGGCCA TGG^CCA
836 TGGCCA
MscI 665 TGGCCA TGG^CCA
836 TGGCCA
NspI 990 GCATGC [GA]CATG^[CT]
1412 GCATGT
StyI 923 CCAAGG C^C[AT][AT]GG
1385 CCTTGG
Tsp509I 1397 AATT ^AATT
1517 AATT
XhoII 176 AGATCT [GA]^GATC[CT]
1708 AGATCT
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeII MboI MboII MnlI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI TspRI Van91I