Restriction map for
pF1KSDA0962
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII BanI BbeI
BbrPI BbvCI BciVI BfrI BglII
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiHKAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV EcoT22I FseI FspI HaeII
HpaI KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 611 GACGCC G[GA]^CG[CT]C
BalI 2106 TGGCCA TGG^CCA
BamHI 2239 GGATCC G^GATCC
BanII 2301 GGGCTC G[GA]GC[CT]^C
BclI 267 TGATCA T^GATCA
BglI 118 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 611 GACGCC G[GA]^CG[CT]C
BseRI 2155 GAGGAG GAGGAG(10/8)
BsgI 2175 GTGCAG GTGCAG(16/14)
BsiEI 1135 CGGTCG CG[GA][CT]^CG
BsmI 521 GCATTC GAATGC(1/-1)
BstXI 1835 CCAGTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1356 GCGC GCG^C
DraI 985 TTTAAA TTT^AAA
EaeI 2106 TGGCCA [CT]^GGCC[GA]
EcoRI 1890 GAATTC G^AATTC
FauI 402 GCGGG CCCGC(4/6)
FbaI 267 TGATCA T^GATCA
HapII 159 CCGG C^CGG
HhaI 1356 GCGC GCG^C
Hin1I 611 GACGCC G[GA]^CG[CT]C
HinP1I 1356 GCGC G^CGC
HindIII 1755 AAGCTT A^AGCTT
HpaII 159 CCGG C^CGG
MfeI 410 CAATTG C^AATTG
MluNI 2106 TGGCCA TGG^CCA
MscI 2106 TGGCCA TGG^CCA
MspI 159 CCGG C^CGG
MunI 410 CAATTG C^AATTG
NspI 1260 GCATGT [GA]CATG^[CT]
PaeR7I 2213 CTCGAG C^TCGAG
SapI 1468 GCTCTTC GCTCTTC(1/4)
ScaI 1154 AGTACT AGT^ACT
SmaI 158 CCCGGG CCC^GGG
StuI 1003 AGGCCT AGG^CCT
XbaI 1980 TCTAGA T^CTAGA
XcmI 1728 CCAAGAGAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2213 CTCGAG C^TCGAG
XmaCI 158 CCCGGG C^CCGGG
XmaI 158 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AccI 1264 GTCTAC GT^[AC][GT]AC
1933 GTCTAC
AvaII 591 GGTCC G^G[AT]CC
1875 GGTCC
BcnI 158 CCCGG CC^[GC]GG
159 CCGGG
BmyI 632 GGGCAC G[AGT]GC[ACT]^C
2301 GGGCTC
BsmBI 278 GAGACG CGTCTC(1/5)
609 GAGACG
Bsp1286I 632 GGGCAC G[AGT]GC[ACT]^C
2301 GGGCTC
HgaI 280 GACGC GACGC(5/10)
611 GACGC
HincII 864 GTTGAC GT[CT]^[GA]AC
2185 GTTGAC
HindII 864 GTTGAC GT[CT]^[GA]AC
2185 GTTGAC
MaeII 776 ACGT A^CGT
1848 ACGT
NciI 158 CCCGG CC^[GC]GG
159 CCGGG
PvuII 1450 CAGCTG CAG^CTG
1953 CAGCTG
SfcI 1266 CTACAG C^T[GA][CT]AG
1912 CTACAG
Tsp45I 1418 GTGAC ^GT[GC]AC
2075 GTGAC
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaI AvrII BbsI
BbvI BcgI BfaI BlnI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII XmnI