Restriction map for
pF1KSDA0967
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BbrPI BciVI
BfrI BlpI Bpu1102I BsaAI BsiWI
BsmBI BsmI BspDI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraIII
DrdI Eam1105I EcoRV EcoT22I FspI
HpaI KpnI MfeI MluI MunI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1393 GACGTC GACGT^C
AccI 2587 GTAGAC GT^[AC][GT]AC
AccIII 2837 TCCGGA T^CCGGA
AflIII 2594 ACGTGT A^C[GA][CT]GT
Aor51HI 881 AGCGCT AGC^GCT
AvrII 3721 CCTAGG C^CTAGG
BamHI 3795 GGATCC G^GATCC
BbeI 1625 GGCGCC GGCGC^C
BbvCI 2891 CCTCAGC CCTCAGC(-5/-2)
BglII 974 AGATCT A^GATCT
BlnI 3721 CCTAGG C^CTAGG
BsaBI 3164 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2837 TCCGGA [AT]^CCGG[AT]
BseAI 2837 TCCGGA T^CCGGA
BsiEI 4685 CGGCCG CG[GA][CT]^CG
BspEI 2837 TCCGGA T^CCGGA
BsrFI 4501 GCCGGC [GA]^CCGG[CT]
BstEII 3052 GGTGACC G^GT[ACGT]ACC
BstPI 3052 GGTGACC G^GT[ACGT]ACC
Cfr10I 4501 GCCGGC [GA]^CCGG[CT]
EagI 4685 CGGCCG C^GGCCG
EclXI 4685 CGGCCG C^GGCCG
Eco47III 881 AGCGCT AGC^GCT
Eco52I 4685 CGGCCG C^GGCCG
EcoO65I 3052 GGTGACC G^GT[ACGT]ACC
EcoRI 2862 GAATTC G^AATTC
FseI 4500 GGCCGGCC GGCCGG^CC
HincII 1477 GTTGAC GT[CT]^[GA]AC
HindII 1477 GTTGAC GT[CT]^[GA]AC
KasI 1625 GGCGCC G^GCGCC
KspI 1791 CCGCGG CCGC^GG
MamI 3164 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2837 TCCGGA T^CCGGA
NaeI 4501 GCCGGC GCC^GGC
NarI 1625 GGCGCC GG^CGCC
NdeI 1184 CATATG CA^TATG
NgoMIV 4501 GCCGGC G^CCGGC
PpuMI 800 AGGACCC [GA]G^G[AT]CC[CT]
SacI 3127 GAGCTC GAGCT^C
SacII 1791 CCGCGG CCGC^GG
SexAI 4487 ACCTGGT A^CC[AT]GGT
SfiI 4677 GGCCCTCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1625 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AcsI 1098 GAATTT [GA]^AATT[CT]
2862 GAATTC
ApoI 1098 GAATTT [GA]^AATT[CT]
2862 GAATTC
BclI 291 TGATCA T^GATCA
4427 TGATCA
Bsp1407I 3178 TGTACA T^GTACA
4583 TGTACA
BsrDI 1239 CATTGC GCAATG(2/0)
3820 CATTGC
BsrGI 3178 TGTACA T^GTACA
4583 TGTACA
DraI 981 TTTAAA TTT^AAA
1149 TTTAAA
FbaI 291 TGATCA T^GATCA
4427 TGATCA
PaeR7I 1812 CTCGAG C^TCGAG
2807 CTCGAG
ScaI 659 AGTACT AGT^ACT
4205 AGTACT
SspBI 3178 TGTACA T^GTACA
4583 TGTACA
XhoI 1812 CTCGAG C^TCGAG
2807 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI