Restriction map for
pF1KSDA0988
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# NO CUT ENZYMES ( 65 enzymes) #
##################################
AclI AflII AgeI Aor51HI ApaI
AscI AseI AsnI Asp700I AvrII
BalI BamHI BbvCI BciVI BfrI
BlnI BsaBI BsiWI BspDI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DrdI Eco47III EcoT22I FseI
HpaI MamI MfeI MluI MluNI
MscI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SbfI ScaI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 950 GGTACC G^GTACC
AccI 2985 GTCGAC GT^[AC][GT]AC
AccIII 3371 TCCGGA T^CCGGA
AhdI 1827 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 950 GGTACC G^GTACC
AspEI 1827 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1873 TGCGCA TGC^GCA
BbeI 2338 GGCGCC GGCGC^C
BclI 2267 TGATCA T^GATCA
BfaI 2956 CTAG C^TAG
BlpI 2284 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2284 GCTGAGC GC^T[ACGT]AGC
BsaI 103 GAGACC GGTCTC(1/5)
BsaWI 3371 TCCGGA [AT]^CCGG[AT]
BseAI 3371 TCCGGA T^CCGGA
BsmI 3032 GCATTC GAATGC(1/-1)
BspEI 3371 TCCGGA T^CCGGA
BsrBI 172 GAGCGG CCGCTC(-3/-3)
BssSI 1309 CTCGTG CACGAG(-5/-1)
BstEII 1701 GGTTACC G^GT[ACGT]ACC
BstPI 1701 GGTTACC G^GT[ACGT]ACC
CelII 2284 GCTGAGC GC^T[ACGT]AGC
DraIII 3471 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 20 CGGCCG C^GGCCG
Eam1105I 1827 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1520 GAAGAG CTCTTC(1/4)
EclXI 20 CGGCCG C^GGCCG
Eco52I 20 CGGCCG C^GGCCG
EcoO65I 1701 GGTTACC G^GT[ACGT]ACC
EcoRI 3240 GAATTC G^AATTC
EcoRV 3250 GATATC GAT^ATC
FbaI 2267 TGATCA T^GATCA
FspI 1873 TGCGCA TGC^GCA
HincII 2985 GTCGAC GT[CT]^[GA]AC
HindII 2985 GTCGAC GT[CT]^[GA]AC
KasI 2338 GGCGCC G^GCGCC
KpnI 950 GGTACC GGTAC^C
Ksp632I 1520 GAAGAG CTCTTC(1/4)
KspI 23 CCGCGG CCGC^GG
MaeI 2956 CTAG C^TAG
MroI 3371 TCCGGA T^CCGGA
NarI 2338 GGCGCC GG^CGCC
NcoI 746 CCATGG C^CATGG
NspI 452 ACATGC [GA]CATG^[CT]
PaeR7I 1470 CTCGAG C^TCGAG
PpuMI 3070 GGGACCC [GA]G^G[AT]CC[CT]
SacII 23 CCGCGG CCGC^GG
SalI 2985 GTCGAC G^TCGAC
SapI 1520 GAAGAGC GCTCTTC(1/4)
SexAI 1697 ACCTGGT A^CC[AT]GGT
SfiI 2718 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2338 GGCGCC GGC^GCC
SmaI 107 CCCGGG CCC^GGG
XhoI 1470 CTCGAG C^TCGAG
XmaCI 107 CCCGGG C^CCGGG
XmaI 107 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 1057 GACGTC GACGT^C
3421 GACGTC
AcsI 310 AAATTT [GA]^AATT[CT]
3240 GAATTC
AflIII 646 ACACGT A^C[GA][CT]GT
1406 ACGTGT
AocI 828 CCTCAGG CC^T[ACGT]AGG
1438 CCTCAGG
ApoI 310 AAATTT [GA]^AATT[CT]
3240 GAATTC
BanII 1178 GAGCCC G[GA]GC[CT]^C
2501 GAGCCC
BbrPI 2812 CACGTG CAC^GTG
3145 CACGTG
BcgI 1751 CGAGGGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2182 GCAGCAGTCTCG
BglII 1839 AGATCT A^GATCT
3083 AGATCT
BsiEI 20 CGGCCG CG[GA][CT]^CG
1121 CGGTCG
BsmBI 1301 CGTCTC CGTCTC(1/5)
2447 GAGACG
Bsp1407I 294 TGTACA T^GTACA
1895 TGTACA
BspHI 369 TCATGA T^CATGA
2024 TCATGA
BspMI 478 ACCTGC ACCTGC(4/8)
2376 GCAGGT
BsrGI 294 TGTACA T^GTACA
1895 TGTACA
Bsu36I 828 CCTCAGG CC^T[ACGT]AGG
1438 CCTCAGG
DraI 307 TTTAAA TTT^AAA
781 TTTAAA
EaeI 20 CGGCCG [CT]^GGCC[GA]
3383 CGGCCA
Eco81I 828 CCTCAGG CC^T[ACGT]AGG
1438 CCTCAGG
EcoNI 126 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
993 CCTCACTCAGG
HindIII 434 AAGCTT A^AGCTT
895 AAGCTT
NaeI 1660 GCCGGC GCC^GGC
2366 GCCGGC
NgoMIV 1660 GCCGGC G^CCGGC
2366 GCCGGC
PmaCI 2812 CACGTG CAC^GTG
3145 CACGTG
PmlI 2812 CACGTG CAC^GTG
3145 CACGTG
RcaI 369 TCATGA T^CATGA
2024 TCATGA
SmlI 1470 CTCGAG C^T[CT][GA]AG
2151 CTCAAG
SspBI 294 TGTACA T^GTACA
1895 TGTACA
SspI 777 AATATT AAT^ATT
2216 AATATT
StuI 1287 AGGCCT AGG^CCT
2891 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI AspHI
AspI AvaI AvaII BanI BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflFI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII