Restriction map for
pF1KSDA0989
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# NO CUT ENZYMES ( 92 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvrII BcgI BciVI BfrI
BglII BlnI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI MfeI MluI MroI
MslI MunI NdeI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI SexAI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI Tsp509I Tth111I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 689 GGTACC G^GTACC
Alw44I 1565 GTGCAC G^TGCAC
AocI 114 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1943 AGCGCT AGC^GCT
ApaLI 1565 GTGCAC G^TGCAC
Asp718I 689 GGTACC G^GTACC
BamHI 734 GGATCC G^GATCC
BbrPI 460 CACGTG CAC^GTG
BclI 1925 TGATCA T^GATCA
BsaAI 460 CACGTG [CT]AC^GT[GA]
BsaWI 2229 ACCGGA [AT]^CCGG[AT]
BsmBI 79 GAGACG CGTCTC(1/5)
BsmI 960 GAATGC GAATGC(1/-1)
BspHI 657 TCATGA T^CATGA
BsrDI 1388 GCAATG GCAATG(2/0)
Bsu36I 114 CCTGAGG CC^T[ACGT]AGG
Eco47III 1943 AGCGCT AGC^GCT
Eco81I 114 CCTGAGG CC^T[ACGT]AGG
FbaI 1925 TGATCA T^GATCA
KpnI 689 GGTACC GGTAC^C
KspI 699 CCGCGG CCGC^GG
MseI 1885 TTAA T^TAA
NaeI 1240 GCCGGC GCC^GGC
NgoMIV 1240 GCCGGC G^CCGGC
NheI 93 GCTAGC G^CTAGC
PaeR7I 1409 CTCGAG C^TCGAG
PflMI 1902 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 460 CACGTG CAC^GTG
PmlI 460 CACGTG CAC^GTG
RcaI 657 TCATGA T^CATGA
SacII 699 CCGCGG CCGC^GG
SbfI 738 CCTGCAGG CCTGCA^GG
ScaI 344 AGTACT AGT^ACT
SphI 720 GCATGC GCATG^C
Sse8387I 738 CCTGCAGG CCTGCA^GG
TfiI 1195 GAATC G^A[AT]TC
Tru9I 1885 TTAA T^TAA
Van91I 1902 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1409 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 35 enzymes) #
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AviII 1392 TGCGCA TGC^GCA
1427 TGCGCA
BalI 1259 TGGCCA TGG^CCA
1854 TGGCCA
BbeI 511 GGCGCC GGCGC^C
1336 GGCGCC
BbsI 13 GAAGAC GAAGAC(2/6)
1119 GAAGAC
BglI 355 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
925 GCCCACCTGGC
BlpI 1626 GCTCAGC GC^T[ACGT]AGC
1774 GCTCAGC
Bpu1102I 1626 GCTCAGC GC^T[ACGT]AGC
1774 GCTCAGC
BpuAI 13 GAAGAC GAAGAC(2/6)
1119 GAAGAC
BsaBI 1190 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2331 GATACTGATC
BsgI 34 CTGCAC GTGCAG(16/14)
2253 GTGCAG
BsrBI 985 GAGCGG CCGCTC(-3/-3)
1459 GAGCGG
BsrFI 275 ACCGGC [GA]^CCGG[CT]
1240 GCCGGC
CelII 1626 GCTCAGC GC^T[ACGT]AGC
1774 GCTCAGC
Cfr10I 275 ACCGGC [GA]^CCGG[CT]
1240 GCCGGC
EarI 2055 CTCTTC CTCTTC(1/4)
1417 GAAGAG
EcoT14I 843 CCATGG C^C[AT][AT]GG
1748 CCATGG
FspI 1392 TGCGCA TGC^GCA
1427 TGCGCA
HphI 244 GGTGA GGTGA(8/7)
1816 TCACC
KasI 511 GGCGCC G^GCGCC
1336 GGCGCC
Ksp632I 2055 CTCTTC CTCTTC(1/4)
1417 GAAGAG
MaeII 461 ACGT A^CGT
1728 ACGT
MamI 1190 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2331 GATACTGATC
MluNI 1259 TGGCCA TGG^CCA
1854 TGGCCA
MscI 1259 TGGCCA TGG^CCA
1854 TGGCCA
NarI 511 GGCGCC GG^CGCC
1336 GGCGCC
NcoI 843 CCATGG C^CATGG
1748 CCATGG
NspI 378 ACATGC [GA]CATG^[CT]
720 GCATGC
SfoI 511 GGCGCC GGC^GCC
1336 GGCGCC
SmaI 408 CCCGGG CCC^GGG
515 CCCGGG
SmlI 1409 CTCGAG C^T[CT][GA]AG
1552 CTCAAG
StyI 843 CCATGG C^C[AT][AT]GG
1748 CCATGG
TaqI 1343 TCGA T^CGA
1410 TCGA
TthHB8I 1343 TCGA T^CGA
1410 TCGA
XmaCI 408 CCCGGG C^CCGGG
515 CCCGGG
XmaI 408 CCCGGG C^CCGGG
515 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI MaeI MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI TseI Tsp45I
TspRI XhoII