Restriction map for
pF1KSDA1014
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AspEI
AspHI AspI AviII AvrII BamHI
BbeI BbrPI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmBI Bsp1407I BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
CfoI Cfr10I CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
MamI MluI MroI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 21 GGTACC G^GTACC
AseI 1636 ATTAAT AT^TAAT
AsnI 1636 ATTAAT AT^TAAT
Asp718I 21 GGTACC G^GTACC
BalI 434 TGGCCA TGG^CCA
BanI 21 GGTACC G^G[CT][GA]CC
BbsI 1138 GAAGAC GAAGAC(2/6)
BbvCI 472 CCTCAGC CCTCAGC(-5/-2)
BciVI 2855 GTATCC GTATCC(6/5)
BpuAI 1138 GAAGAC GAAGAC(2/6)
BsiEI 27 CGGCCG CG[GA][CT]^CG
BsmI 1334 GAATGC GAATGC(1/-1)
BspDI 454 ATCGAT AT^CGAT
BspHI 278 TCATGA T^CATGA
BspMI 2604 ACCTGC ACCTGC(4/8)
BsrDI 2660 CATTGC GCAATG(2/0)
BstEII 2464 GGTCACC G^GT[ACGT]ACC
BstPI 2464 GGTCACC G^GT[ACGT]ACC
BstXI 591 CCAAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 454 ATCGAT AT^CGAT
EagI 27 CGGCCG C^GGCCG
EclXI 27 CGGCCG C^GGCCG
Eco52I 27 CGGCCG C^GGCCG
EcoO65I 2464 GGTCACC G^GT[ACGT]ACC
EcoRV 1306 GATATC GAT^ATC
HincII 411 GTCAAC GT[CT]^[GA]AC
HindII 411 GTCAAC GT[CT]^[GA]AC
KpnI 21 GGTACC GGTAC^C
MaeII 1299 ACGT A^CGT
MluNI 434 TGGCCA TGG^CCA
MscI 434 TGGCCA TGG^CCA
PflMI 1438 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1636 ATTAAT AT^TAAT
RcaI 278 TCATGA T^CATGA
SmaI 14 CCCGGG CCC^GGG
SmlI 1325 CTCAAG C^T[CT][GA]AG
SpeI 805 ACTAGT A^CTAGT
Van91I 1438 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 14 CCCGGG C^CCGGG
XmaI 14 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AocI 991 CCTGAGG CC^T[ACGT]AGG
3001 CCTGAGG
Asp700I 916 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2992 GAAGCCCTTC
BanII 1712 GGGCTC G[GA]GC[CT]^C
2245 GAGCCC
BglI 148 GCCCCGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
181 GCCGCCGCGGC
BmyI 1712 GGGCTC G[AGT]GC[ACT]^C
2245 GAGCCC
BsgI 2918 CTGCAC GTGCAG(16/14)
235 GTGCAG
Bsp1286I 1712 GGGCTC G[AGT]GC[ACT]^C
2245 GAGCCC
BstAPI 1456 GCAATTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2599 GCAGCACCTGC
BstYI 1155 GGATCT [GA]^GATC[CT]
2207 AGATCC
Bsu36I 991 CCTGAGG CC^T[ACGT]AGG
3001 CCTGAGG
DraII 60 GGGTCCT [GA]G^G[ACGT]CC[CT]
86 GGGACCC
EaeI 27 CGGCCG [CT]^GGCC[GA]
434 TGGCCA
Eco81I 991 CCTGAGG CC^T[ACGT]AGG
3001 CCTGAGG
EcoO109I 60 GGGTCCT [GA]G^G[ACGT]CC[CT]
86 GGGACCC
EcoRI 920 GAATTC G^AATTC
1491 GAATTC
FauI 145 CCCGC CCCGC(4/6)
1704 GCGGG
MfeI 1457 CAATTG C^AATTG
2639 CAATTG
MflI 1155 GGATCT [GA]^GATC[CT]
2207 AGATCC
MslI 168 CACCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2391 CACTGCAGTG
MunI 1457 CAATTG C^AATTG
2639 CAATTG
PpuMI 60 GGGTCCT [GA]G^G[AT]CC[CT]
86 GGGACCC
PvuII 296 CAGCTG CAG^CTG
2953 CAGCTG
XhoII 1155 GGATCT [GA]^GATC[CT]
2207 AGATCC
XmnI 916 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2992 GAAGCCCTTC
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaI AvaII
BbvI BcnI BfaI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI BsrI BssKI BstF5I BstNI
BstUI Cac8I Cfr13I DdeI DpnII
DsaI EarI EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I