Restriction map for
pF1KSDA1020
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AvrII BalI BamHI BbvCI
BciVI BclI BfrI BlnI BsaBI
BsaI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluNI MroI MscI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 139 GACGTC GACGT^C
Alw44I 1744 GTGCAC G^TGCAC
AlwI 918 GGATC GGATC(4/5)
ApaLI 1744 GTGCAC G^TGCAC
BbeI 960 GGCGCC GGCGC^C
BbrPI 1378 CACGTG CAC^GTG
BcgI 993 GCACTCCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 284 AGATCT A^GATCT
BsaAI 1378 CACGTG [CT]AC^GT[GA]
BsaWI 23 TCCGGT [AT]^CCGG[AT]
BsgI 226 CTGCAC GTGCAG(16/14)
BsmBI 1297 GAGACG CGTCTC(1/5)
BsmI 1331 GCATTC GAATGC(1/-1)
BspLU11I 1274 ACATGT A^CATGT
BspMI 1820 ACCTGC ACCTGC(4/8)
DraIII 1478 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1254 CGGCCA [CT]^GGCC[GA]
EcoNI 951 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI 1386 GACTC G^A[ACGT]TC
KasI 960 GGCGCC G^GCGCC
MluI 1645 ACGCGT A^CGCGT
NaeI 424 GCCGGC GCC^GGC
NarI 960 GGCGCC GG^CGCC
NcoI 914 CCATGG C^CATGG
NgoMIV 424 GCCGGC G^CCGGC
PleI 1386 GACTC GAGTC(4/5)
PmaCI 1378 CACGTG CAC^GTG
PmlI 1378 CACGTG CAC^GTG
SacI 568 GAGCTC GAGCT^C
SfoI 960 GGCGCC GGC^GCC
SmaI 1083 CCCGGG CCC^GGG
SmlI 415 CTCAAG C^T[CT][GA]AG
TaqI 85 TCGA T^CGA
TthHB8I 85 TCGA T^CGA
XcmI 1121 CCAATGGCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1083 CCCGGG C^CCGGG
XmaI 1083 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 139 GACGTC G[GA]^CG[CT]C
960 GGCGCC
AfaI 1278 GTAC GT^AC
1719 GTAC
AflIII 1274 ACATGT A^C[GA][CT]GT
1645 ACGCGT
Asp700I 598 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1674 GAAGCCCTTC
AspI 256 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1299 GACGGTGTC
BlpI 816 GCTGAGC GC^T[ACGT]AGC
1498 GCTGAGC
Bpu1102I 816 GCTGAGC GC^T[ACGT]AGC
1498 GCTGAGC
BsaHI 139 GACGTC G[GA]^CG[CT]C
960 GGCGCC
BsrBI 1051 GAGCGG CCGCTC(-3/-3)
1233 GAGCGG
BsrFI 424 GCCGGC [GA]^CCGG[CT]
1280 ACCGGC
BssSI 1567 CACGAG CACGAG(-5/-1)
532 CTCGTG
BstXI 458 CCACTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
914 CCATGGATCTGG
BstYI 284 AGATCT [GA]^GATC[CT]
918 GGATCT
CelII 816 GCTGAGC GC^T[ACGT]AGC
1498 GCTGAGC
Cfr10I 424 GCCGGC [GA]^CCGG[CT]
1280 ACCGGC
DpnII 285 GATC ^GATC
919 GATC
DsaI 497 CCACGG C^C[GA][CT]GG
914 CCATGG
HaeII 960 GGCGCC [GA]GCGC^[CT]
1264 AGCGCC
Hin1I 139 GACGTC G[GA]^CG[CT]C
960 GGCGCC
MaeIII 137 GTGAC ^GT[ACGT]AC
1649 GTCAC
MboI 285 GATC ^GATC
919 GATC
MflI 284 AGATCT [GA]^GATC[CT]
918 GGATCT
NdeII 285 GATC ^GATC
919 GATC
PflFI 256 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1299 GACGGTGTC
PstI 100 CTGCAG CTGCA^G
687 CTGCAG
RsaI 1278 GTAC GT^AC
1719 GTAC
SapI 981 GAAGAGC GCTCTTC(1/4)
1399 GAAGAGC
Sau3AI 285 GATC ^GATC
919 GATC
SfcI 100 CTGCAG C^T[GA][CT]AG
687 CTGCAG
SphI 1700 GCATGC GCATG^C
1826 GCATGC
Tsp45I 137 GTGAC ^GT[GC]AC
1649 GTCAC
Tth111I 256 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1299 GACGGTGTC
XhoII 284 AGATCT [GA]^GATC[CT]
918 GGATCT
XmnI 598 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1674 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AluI AlwNI ApaI
AspHI AvaI AvaII AviII BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeI MaeII MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV NspI
PpuMI PvuII Sau96I ScrFI SfaNI
StyI TseI TspRI