Restriction map for pF1KSDA1036
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AviII       AvrII       BbeI        BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BsaBI       BseAI       BsiWI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraI        Eam1105I    Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KasI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspI        
StuI        SwaI        TfiI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I       1079  GGTACC               G^GTACC
AccI          342  GTCTAC               GT^[AC][GT]AC
AcsI          527  GAATTT               [GA]^AATT[CT]
AcyI          169  GGCGTC               G[GA]^CG[CT]C
AflIII        237  ACACGT               A^C[GA][CT]GT
ApoI          527  GAATTT               [GA]^AATT[CT]
Asp700I       597  GAACTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1079  GGTACC               G^GTACC
AvaI         1029  CTCGGG               C^[CT]CG[GA]G
BamHI         281  GGATCC               G^GATCC
BbsI         1041  GAAGAC               GAAGAC(2/6)
BbvCI         110  CCTCAGC              CCTCAGC(-5/-2)
BlpI          705  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      705  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1041  GAAGAC               GAAGAC(2/6)
BsaHI         169  GGCGTC               G[GA]^CG[CT]C
BsaI           94  GAGACC               GGTCTC(1/5)
BsaWI         950  ACCGGA               [AT]^CCGG[AT]
BseRI         127  GAGGAG               GAGGAG(10/8)
BsgI          373  GTGCAG               GTGCAG(16/14)
BsiEI         741  CGGCCG               CG[GA][CT]^CG
BsmBI         161  GAGACG               CGTCTC(1/5)
BsoBI        1029  CTCGGG               C^[CT]CG[GA]G
Bsp1407I      680  TGTACA               T^GTACA
BsrGI         680  TGTACA               T^GTACA
BsrI          984  CCAGT                ACTGG(1/-1)
BssSI         715  CTCGTG               CACGAG(-5/-1)
BstAPI        828  GCACTCGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         252  CCACCCCGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         705  GCTCAGC              GC^T[ACGT]AGC
DrdI          163  GACGGAGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          285  CCGTGG               C^C[GA][CT]GG
EagI          741  CGGCCG               C^GGCCG
EclXI         741  CGGCCG               C^GGCCG
Eco52I        741  CGGCCG               C^GGCCG
HgaI          170  GCGTC                GACGC(5/10)
Hin1I         169  GGCGTC               G[GA]^CG[CT]C
HincII        184  GTCAAC               GT[CT]^[GA]AC
HindII        184  GTCAAC               GT[CT]^[GA]AC
HinfI         660  GAGTC                G^A[ACGT]TC
KpnI         1079  GGTACC               GGTAC^C
MslI          253  CACCCCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI          660  GAGTC                GAGTC(4/5)
PstI          394  CTGCAG               CTGCA^G
SacI          712  GAGCTC               GAGCT^C
SapI          435  GAAGAGC              GCTCTTC(1/4)
SfcI          394  CTGCAG               C^T[GA][CT]AG
SphI          545  GCATGC               GCATG^C
SspBI         680  TGTACA               T^GTACA
TspRI        1014  CAGTG                CA[GC]TG[ACGT][ACGT]^
XmnI          597  GAACTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Aor51HI       377  AGCGCT               AGC^GCT
              560  AGCGCT              
ApaI          927  GGGCCC               GGGCC^C
              980  GGGCCC              
AspHI         712  GAGCTC               G[AT]GC[AT]^C
              757  GTGCTC              
BalI          242  TGGCCA               TGG^CCA
              467  TGGCCA              
BanI           40  GGTGCC               G^G[CT][GA]CC
             1079  GGTACC              
BbrPI         238  CACGTG               CAC^GTG
              754  CACGTG              
BcnI          189  CCGGG                CC^[GC]GG
             1089  CCGGG               
BpmI          556  CTGGAG               CTGGAG(16/14)
              880  CTGGAG              
BsaAI         238  CACGTG               [CT]AC^GT[GA]
              754  CACGTG              
BsiHKAI       712  GAGCTC               G[AT]GC[AT]^C
              757  GTGCTC              
BsmAI          94  GAGAC                GTCTC(1/5)
              161  GAGAC               
BspMI         155  ACCTGC               ACCTGC(4/8)
              299  ACCTGC              
Eco47III      377  AGCGCT               AGC^GCT
              560  AGCGCT              
EcoT14I       470  CCAAGG               C^C[AT][AT]GG
              944  CCAAGG              
MaeIII        789  GTCAC                ^GT[ACGT]AC
             1034  GTGAC               
MluNI         242  TGGCCA               TGG^CCA
              467  TGGCCA              
MscI          242  TGGCCA               TGG^CCA
              467  TGGCCA              
MseI          431  TTAA                 T^TAA
             1073  TTAA                
NciI          189  CCGGG                CC^[GC]GG
             1089  CCGGG               
NspI          545  GCATGC               [GA]CATG^[CT]
              899  ACATGC              
PmaCI         238  CACGTG               CAC^GTG
              754  CACGTG              
PmlI          238  CACGTG               CAC^GTG
              754  CACGTG              
PpuMI         152  AGGACCT              [GA]G^G[AT]CC[CT]
              671  AGGACCT             
SmlI          760  CTCAAG               C^T[CT][GA]AG
              907  CTCAAG              
StyI          470  CCAAGG               C^C[AT][AT]GG
              944  CCAAGG              
TaqI          728  TCGA                 T^CGA
              818  TCGA                
Tru9I         431  TTAA                 T^TAA
             1073  TTAA                
Tsp45I        789  GTCAC                ^GT[GC]AC
             1034  GTGAC               
Tsp509I       429  AATT                 ^AATT
              528  AATT                
TthHB8I       728  TCGA                 T^CGA
              818  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaII       BanII       BbvI        BmyI        BsaJI       
BsiYI       BslI        BsmFI       Bsp1286I    BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DraIII      EaeI        EarI        EcoNI       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        ItaI        Ksp632I     MaeII       MboI        
MboII       MflI        MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
TseI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com