################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BbeI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BsaBI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KspI MaeI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 1079 GGTACC G^GTACC AccI 342 GTCTAC GT^[AC][GT]AC AcsI 527 GAATTT [GA]^AATT[CT] AcyI 169 GGCGTC G[GA]^CG[CT]C AflIII 237 ACACGT A^C[GA][CT]GT ApoI 527 GAATTT [GA]^AATT[CT] Asp700I 597 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1079 GGTACC G^GTACC AvaI 1029 CTCGGG C^[CT]CG[GA]G BamHI 281 GGATCC G^GATCC BbsI 1041 GAAGAC GAAGAC(2/6) BbvCI 110 CCTCAGC CCTCAGC(-5/-2) BlpI 705 GCTCAGC GC^T[ACGT]AGC Bpu1102I 705 GCTCAGC GC^T[ACGT]AGC BpuAI 1041 GAAGAC GAAGAC(2/6) BsaHI 169 GGCGTC G[GA]^CG[CT]C BsaI 94 GAGACC GGTCTC(1/5) BsaWI 950 ACCGGA [AT]^CCGG[AT] BseRI 127 GAGGAG GAGGAG(10/8) BsgI 373 GTGCAG GTGCAG(16/14) BsiEI 741 CGGCCG CG[GA][CT]^CG BsmBI 161 GAGACG CGTCTC(1/5) BsoBI 1029 CTCGGG C^[CT]CG[GA]G Bsp1407I 680 TGTACA T^GTACA BsrGI 680 TGTACA T^GTACA BsrI 984 CCAGT ACTGG(1/-1) BssSI 715 CTCGTG CACGAG(-5/-1) BstAPI 828 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 252 CCACCCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 705 GCTCAGC GC^T[ACGT]AGC DrdI 163 GACGGAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 285 CCGTGG C^C[GA][CT]GG EagI 741 CGGCCG C^GGCCG EclXI 741 CGGCCG C^GGCCG Eco52I 741 CGGCCG C^GGCCG HgaI 170 GCGTC GACGC(5/10) Hin1I 169 GGCGTC G[GA]^CG[CT]C HincII 184 GTCAAC GT[CT]^[GA]AC HindII 184 GTCAAC GT[CT]^[GA]AC HinfI 660 GAGTC G^A[ACGT]TC KpnI 1079 GGTACC GGTAC^C MslI 253 CACCCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PleI 660 GAGTC GAGTC(4/5) PstI 394 CTGCAG CTGCA^G SacI 712 GAGCTC GAGCT^C SapI 435 GAAGAGC GCTCTTC(1/4) SfcI 394 CTGCAG C^T[GA][CT]AG SphI 545 GCATGC GCATG^C SspBI 680 TGTACA T^GTACA TspRI 1014 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 597 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Aor51HI 377 AGCGCT AGC^GCT 560 AGCGCT ApaI 927 GGGCCC GGGCC^C 980 GGGCCC AspHI 712 GAGCTC G[AT]GC[AT]^C 757 GTGCTC BalI 242 TGGCCA TGG^CCA 467 TGGCCA BanI 40 GGTGCC G^G[CT][GA]CC 1079 GGTACC BbrPI 238 CACGTG CAC^GTG 754 CACGTG BcnI 189 CCGGG CC^[GC]GG 1089 CCGGG BpmI 556 CTGGAG CTGGAG(16/14) 880 CTGGAG BsaAI 238 CACGTG [CT]AC^GT[GA] 754 CACGTG BsiHKAI 712 GAGCTC G[AT]GC[AT]^C 757 GTGCTC BsmAI 94 GAGAC GTCTC(1/5) 161 GAGAC BspMI 155 ACCTGC ACCTGC(4/8) 299 ACCTGC Eco47III 377 AGCGCT AGC^GCT 560 AGCGCT EcoT14I 470 CCAAGG C^C[AT][AT]GG 944 CCAAGG MaeIII 789 GTCAC ^GT[ACGT]AC 1034 GTGAC MluNI 242 TGGCCA TGG^CCA 467 TGGCCA MscI 242 TGGCCA TGG^CCA 467 TGGCCA MseI 431 TTAA T^TAA 1073 TTAA NciI 189 CCGGG CC^[GC]GG 1089 CCGGG NspI 545 GCATGC [GA]CATG^[CT] 899 ACATGC PmaCI 238 CACGTG CAC^GTG 754 CACGTG PmlI 238 CACGTG CAC^GTG 754 CACGTG PpuMI 152 AGGACCT [GA]G^G[AT]CC[CT] 671 AGGACCT SmlI 760 CTCAAG C^T[CT][GA]AG 907 CTCAAG StyI 470 CCAAGG C^C[AT][AT]GG 944 CCAAGG TaqI 728 TCGA T^CGA 818 TCGA Tru9I 431 TTAA T^TAA 1073 TTAA Tsp45I 789 GTCAC ^GT[GC]AC 1034 GTGAC Tsp509I 429 AATT ^AATT 528 AATT TthHB8I 728 TCGA T^CGA 818 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BanII BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI XhoII