Restriction map for
pF1KSDA1045
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I CelII ClaI CpoI
DpnII DraI DraIII DrdI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MboI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NarI NcoI NdeI NdeII
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AfaI 163 GTAC GT^AC
AhdI 799 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 441 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 268 CCTGAGG CC^T[ACGT]AGG
AspEI 799 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 924 GAGCAC G[AT]GC[AT]^C
BcgI 187 GCAGGAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 613 GAGACC GGTCTC(1/5)
BsiEI 222 CGGCCG CG[GA][CT]^CG
BsiHKAI 924 GAGCAC G[AT]GC[AT]^C
BspMI 36 GCAGGT ACCTGC(4/8)
BsrFI 220 GCCGGC [GA]^CCGG[CT]
BstXI 1103 CCAGATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 268 CCTGAGG CC^T[ACGT]AGG
Cfr10I 220 GCCGGC [GA]^CCGG[CT]
DsaI 132 CCGTGG C^C[GA][CT]GG
EaeI 222 CGGCCG [CT]^GGCC[GA]
EagI 222 CGGCCG C^GGCCG
Eam1105I 799 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 222 CGGCCG C^GGCCG
Eco52I 222 CGGCCG C^GGCCG
Eco81I 268 CCTGAGG CC^T[ACGT]AGG
EcoNI 717 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 495 CCAAGG C^C[AT][AT]GG
HincII 382 GTCAAC GT[CT]^[GA]AC
HindII 382 GTCAAC GT[CT]^[GA]AC
MslI 556 CACTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 220 GCCGGC GCC^GGC
NgoMIV 220 GCCGGC G^CCGGC
PvuII 419 CAGCTG CAG^CTG
RsaI 163 GTAC GT^AC
SapI 733 GAAGAGC GCTCTTC(1/4)
SfaNI 1010 GCATC GCATC(5/9)
StuI 91 AGGCCT AGG^CCT
StyI 495 CCAAGG C^C[AT][AT]GG
TfiI 293 GATTC G^A[AT]TC
XcmI 320 CCACCCGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccII 226 CGCG CG^CG
256 CGCG
ApaI 713 GGGCCC GGGCC^C
953 GGGCCC
AvaI 893 CCCGAG C^[CT]CG[GA]G
911 CTCGGG
BcnI 323 CCCGG CC^[GC]GG
951 CCGGG
BglI 479 GCCGCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
901 GCCTTCCTGGC
BsmAI 500 GAGAC GTCTC(1/5)
613 GAGAC
BsoBI 893 CCCGAG C^[CT]CG[GA]G
911 CTCGGG
BsrBI 238 GAGCGG CCGCTC(-3/-3)
880 GAGCGG
BstAPI 440 GCAGGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
831 GCAGCAGTTGC
BstUI 226 CGCG CG^CG
256 CGCG
HgaI 104 GACGC GACGC(5/10)
840 GCGTC
MvnI 226 CGCG CG^CG
256 CGCG
NciI 323 CCCGG CC^[GC]GG
951 CCGGG
PpuMI 124 GGGTCCC [GA]G^G[AT]CC[CT]
1023 GGGTCCC
PstI 829 CTGCAG CTGCA^G
999 CTGCAG
TaqI 291 TCGA T^CGA
777 TCGA
TthHB8I 291 TCGA T^CGA
777 TCGA
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DraII EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboII
MnlI MspA1I MspI MvaI MwoI
NlaIII NlaIV PleI Sau96I ScrFI
SfcI TseI Tsp45I TspRI