Restriction map for
pF1KSDA1048
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AflIII AgeI Alw44I ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BanI BbeI BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco52I
EcoO65I EcoRI EcoRV FbaI FseI
FspI KasI KpnI KspI MamI
MluI MroI MvnI NarI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 2076 GTCTAC GT^[AC][GT]AC
AclI 2053 AACGTT AA^CGTT
AcyI 1332 GACGCC G[GA]^CG[CT]C
AhdI 128 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1198 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1158 AGCGCT AGC^GCT
Asp700I 6 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 128 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1210 GGATCC G^GATCC
BbsI 112 GTCTTC GAAGAC(2/6)
BbvCI 1981 GCTGAGG CCTCAGC(-5/-2)
BglI 1015 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2189 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2189 GCTCAGC GC^T[ACGT]AGC
BpuAI 112 GTCTTC GAAGAC(2/6)
BsaAI 2079 TACGTG [CT]AC^GT[GA]
BsaHI 1332 GACGCC G[GA]^CG[CT]C
BsgI 1925 GTGCAG GTGCAG(16/14)
BspMI 132 GCAGGT ACCTGC(4/8)
BsrBI 1361 CCGCTC CCGCTC(-3/-3)
BsrDI 197 GCAATG GCAATG(2/0)
BsrFI 1452 GCCGGC [GA]^CCGG[CT]
BstAPI 1575 GCAGCTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1881 CCAACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1198 CCTCAGG CC^T[ACGT]AGG
CelII 2189 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1452 GCCGGC [GA]^CCGG[CT]
Eam1105I 128 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1158 AGCGCT AGC^GCT
Eco81I 1198 CCTCAGG CC^T[ACGT]AGG
EcoNI 2065 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2497 ATGCAT ATGCA^T
HgaI 1332 GACGC GACGC(5/10)
Hin1I 1332 GACGCC G[GA]^CG[CT]C
HpaI 2539 GTTAAC GTT^AAC
MslI 369 CATTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1452 GCCGGC GCC^GGC
NgoMIV 1452 GCCGGC G^CCGGC
NsiI 2497 ATGCAT ATGCA^T
PflMI 2544 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2053 AACGTT AA^CGTT
SexAI 914 ACCAGGT A^CC[AT]GGT
SspI 304 AATATT AAT^ATT
StuI 1105 AGGCCT AGG^CCT
Van91I 2544 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 6 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 601 AAATTC [GA]^AATT[CT]
1587 GAATTT
ApoI 601 AAATTC [GA]^AATT[CT]
1587 GAATTT
AvaI 81 CTCGGG C^[CT]CG[GA]G
2173 CCCGAG
BalI 393 TGGCCA TGG^CCA
1640 TGGCCA
BanII 48 GGGCTC G[GA]GC[CT]^C
737 GGGCTC
BfaI 326 CTAG C^TAG
2536 CTAG
BsmAI 1081 GAGAC GTCTC(1/5)
1991 GTCTC
BsoBI 81 CTCGGG C^[CT]CG[GA]G
2173 CCCGAG
Bsp1407I 698 TGTACA T^GTACA
969 TGTACA
BsrGI 698 TGTACA T^GTACA
969 TGTACA
DraII 524 GGGACCT [GA]G^G[ACGT]CC[CT]
1937 GGGTCCC
EaeI 393 TGGCCA [CT]^GGCC[GA]
1640 TGGCCA
EcoO109I 524 GGGACCT [GA]G^G[ACGT]CC[CT]
1937 GGGTCCC
MaeI 326 CTAG C^TAG
2536 CTAG
MfeI 1088 CAATTG C^AATTG
1532 CAATTG
MluNI 393 TGGCCA TGG^CCA
1640 TGGCCA
MscI 393 TGGCCA TGG^CCA
1640 TGGCCA
MunI 1088 CAATTG C^AATTG
1532 CAATTG
NspI 224 GCATGT [GA]CATG^[CT]
854 ACATGC
PpuMI 524 GGGACCT [GA]G^G[AT]CC[CT]
1937 GGGTCCC
SspBI 698 TGTACA T^GTACA
969 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII