Restriction map for
pF1KSDA1059
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# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspI AviII AvrII BbvCI
BciVI BfrI BlnI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI Eco47III EcoO65I EcoRI EcoRV
FseI FspI HpaI KpnI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SfiI SfuI
SnaBI SphI SspBI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 3270 AAATTT [GA]^AATT[CT]
AhdI 117 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3270 AAATTT [GA]^AATT[CT]
Asp700I 2154 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 117 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1115 GGATCC G^GATCC
BbrPI 2641 CACGTG CAC^GTG
BcgI 554 GCACCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 836 TGATCA T^GATCA
BglII 1808 AGATCT A^GATCT
BlpI 3371 GCTCAGC GC^T[ACGT]AGC
BpmI 3351 CTCCAG CTGGAG(16/14)
Bpu1102I 3371 GCTCAGC GC^T[ACGT]AGC
BsaBI 3456 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2952 GAATGC GAATGC(1/-1)
BssHII 10 GCGCGC G^CGCGC
BssSI 1858 CTCGTG CACGAG(-5/-1)
CelII 3371 GCTCAGC GC^T[ACGT]AGC
DraI 3436 TTTAAA TTT^AAA
DraIII 3542 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 449 CGGCCG C^GGCCG
Eam1105I 117 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 449 CGGCCG C^GGCCG
Eco52I 449 CGGCCG C^GGCCG
EcoT22I 994 ATGCAT ATGCA^T
FbaI 836 TGATCA T^GATCA
HindIII 370 AAGCTT A^AGCTT
MamI 3456 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1396 ACGCGT A^CGCGT
NsiI 994 ATGCAT ATGCA^T
PmaCI 2641 CACGTG CAC^GTG
PmlI 2641 CACGTG CAC^GTG
PshAI 78 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1226 GAAGAGC GCTCTTC(1/4)
SbfI 1109 CCTGCAGG CCTGCA^GG
ScaI 1286 AGTACT AGT^ACT
SgrAI 158 CACCGGCG C[GA]^CCGG[CT]G
SmaI 491 CCCGGG CCC^GGG
SmlI 3470 CTCAAG C^T[CT][GA]AG
Sse8387I 1109 CCTGCAGG CCTGCA^GG
StuI 1259 AGGCCT AGG^CCT
TfiI 1688 GAATC G^A[AT]TC
XmaCI 491 CCCGGG C^CCGGG
XmaI 491 CCCGGG C^CCGGG
XmnI 2154 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 75 GTCGAC GT^[AC][GT]AC
696 GTCGAC
AccIII 53 TCCGGA T^CCGGA
1587 TCCGGA
AlwNI 359 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
892 CAGAGCCTG
AocI 1679 CCTCAGG CC^T[ACGT]AGG
3562 CCTGAGG
AvaI 491 CCCGGG C^[CT]CG[GA]G
2462 CTCGGG
BsaWI 53 TCCGGA [AT]^CCGG[AT]
1587 TCCGGA
BseAI 53 TCCGGA T^CCGGA
1587 TCCGGA
BsiEI 449 CGGCCG CG[GA][CT]^CG
2483 CGACCG
BsoBI 491 CCCGGG C^[CT]CG[GA]G
2462 CTCGGG
BspEI 53 TCCGGA T^CCGGA
1587 TCCGGA
BspHI 2003 TCATGA T^CATGA
2988 TCATGA
BstAPI 987 GCAACTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1617 GCACTGCCTGC
BstXI 2302 CCAGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2816 CCAGTCAACTGG
Bsu36I 1679 CCTCAGG CC^T[ACGT]AGG
3562 CCTGAGG
DrdI 2704 GACACAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3589 GACACGCGGGTC
Eco81I 1679 CCTCAGG CC^T[ACGT]AGG
3562 CCTGAGG
KspI 181 CCGCGG CCGC^GG
511 CCGCGG
MfeI 2044 CAATTG C^AATTG
2244 CAATTG
MroI 53 TCCGGA T^CCGGA
1587 TCCGGA
MunI 2044 CAATTG C^AATTG
2244 CAATTG
NaeI 91 GCCGGC GCC^GGC
2078 GCCGGC
NgoMIV 91 GCCGGC G^CCGGC
2078 GCCGGC
PstI 1110 CTGCAG CTGCA^G
2340 CTGCAG
PvuII 2708 CAGCTG CAG^CTG
3325 CAGCTG
RcaI 2003 TCATGA T^CATGA
2988 TCATGA
SacII 181 CCGCGG CCGC^GG
511 CCGCGG
SalI 75 GTCGAC G^TCGAC
696 GTCGAC
SexAI 1058 ACCTGGT A^CC[AT]GGT
1529 ACCAGGT
SpeI 1219 ACTAGT A^CTAGT
3658 ACTAGT
SspI 2157 AATATT AAT^ATT
2993 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI ApaLI AspHI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII