Restriction map for
pF1KSDA1066
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# NO CUT ENZYMES ( 69 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp718I BcgI BciVI BfrI BglII
BsaWI BseAI BsmI BspDI BspEI
BspHI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eco47III EcoNI
EcoRV EcoT22I FseI HpaI KpnI
MfeI MluI MroI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SbfI
ScaI SexAI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AocI 3330 CCTGAGG CC^T[ACGT]AGG
Asp700I 624 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2787 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1209 CCTAGG C^CTAGG
BamHI 2321 GGATCC G^GATCC
BbeI 3589 GGCGCC GGCGC^C
BbsI 2767 GTCTTC GAAGAC(2/6)
BbvCI 1371 GCTGAGG CCTCAGC(-5/-2)
BlnI 1209 CCTAGG C^CTAGG
BpuAI 2767 GTCTTC GAAGAC(2/6)
BsaBI 3 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1066 GAGACC GGTCTC(1/5)
BsiWI 3432 CGTACG C^GTACG
Bsp1407I 1525 TGTACA T^GTACA
BspLU11I 1055 ACATGT A^CATGT
BsrDI 2738 CATTGC GCAATG(2/0)
BsrGI 1525 TGTACA T^GTACA
BstAPI 1362 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 960 GGTCACC G^GT[ACGT]ACC
BstPI 960 GGTCACC G^GT[ACGT]ACC
Bsu36I 3330 CCTGAGG CC^T[ACGT]AGG
DrdI 1161 GACGGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3330 CCTGAGG CC^T[ACGT]AGG
EcoO65I 960 GGTCACC G^GT[ACGT]ACC
EcoRI 1879 GAATTC G^AATTC
HindIII 1118 AAGCTT A^AGCTT
KasI 3589 GGCGCC G^GCGCC
MamI 3 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3589 GGCGCC GG^CGCC
NotI 784 GCGGCCGC GC^GGCCGC
PflFI 2787 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1742 GCTCTTC GCTCTTC(1/4)
SfiI 2682 GGCCACAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3589 GGCGCC GGC^GCC
SspBI 1525 TGTACA T^GTACA
StuI 1985 AGGCCT AGG^CCT
Tth111I 2787 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 624 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AcsI 319 AAATTC [GA]^AATT[CT]
1879 GAATTC
ApaI 2713 GGGCCC GGGCC^C
3789 GGGCCC
ApoI 319 AAATTC [GA]^AATT[CT]
1879 GAATTC
AviII 299 TGCGCA TGC^GCA
974 TGCGCA
BclI 1661 TGATCA T^GATCA
2357 TGATCA
BglI 92 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2683 GCCACAGAGGC
BssSI 226 CACGAG CACGAG(-5/-1)
676 CTCGTG
DraIII 226 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2907 CACCATGTG
FbaI 1661 TGATCA T^GATCA
2357 TGATCA
FspI 299 TGCGCA TGC^GCA
974 TGCGCA
NspI 1055 ACATGT [GA]CATG^[CT]
3018 GCATGT
SacI 160 GAGCTC GAGCT^C
2284 GAGCTC
Tsp509I 320 AATT ^AATT
1880 AATT
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# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AhdI AluI Alw44I AlwI
AlwNI ApaLI AspEI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PmaCI PmlI PpuMI PshAI
PstI PvuII RsaI SacII SalI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI