Restriction map for
pF1KSDA1074
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# NO CUT ENZYMES ( 95 enzymes) #
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AatII Acc65I AccIII AclI AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BglI BlnI BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT22I FseI
FspI HpaI KpnI KspI MluI
MluNI MroI MscI NaeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 97 GGCGCC G[GA]^CG[CT]C
AflII 732 CTTAAG C^TTAAG
Alw44I 1186 GTGCAC G^TGCAC
ApaLI 1186 GTGCAC G^TGCAC
AvaI 2594 CTCGAG C^[CT]CG[GA]G
BanI 97 GGCGCC G^G[CT][GA]CC
BanII 39 GGGCTC G[GA]GC[CT]^C
BbeI 97 GGCGCC GGCGC^C
BfrI 732 CTTAAG C^TTAAG
BlpI 830 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 830 GCTTAGC GC^T[ACGT]AGC
BsaHI 97 GGCGCC G[GA]^CG[CT]C
BsaI 128 GAGACC GGTCTC(1/5)
BsgI 184 GTGCAG GTGCAG(16/14)
BsmFI 817 GTCCC GGGAC(10/14)
BsoBI 2594 CTCGAG C^[CT]CG[GA]G
BspHI 2706 TCATGA T^CATGA
BspMI 4243 GCAGGT ACCTGC(4/8)
BssSI 3243 CACGAG CACGAG(-5/-1)
BstXI 2913 CCAGTATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 830 GCTTAGC GC^T[ACGT]AGC
DraIII 4823 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 731 CCTTAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1475 GATATC GAT^ATC
HaeII 97 GGCGCC [GA]GCGC^[CT]
HgaI 2575 GACGC GACGC(5/10)
Hin1I 97 GGCGCC G[GA]^CG[CT]C
HincII 4744 GTCAAC GT[CT]^[GA]AC
HindII 4744 GTCAAC GT[CT]^[GA]AC
KasI 97 GGCGCC G^GCGCC
MfeI 3802 CAATTG C^AATTG
MunI 3802 CAATTG C^AATTG
NarI 97 GGCGCC GG^CGCC
NdeI 884 CATATG CA^TATG
NheI 4734 GCTAGC G^CTAGC
PaeR7I 2594 CTCGAG C^TCGAG
RcaI 2706 TCATGA T^CATGA
SfoI 97 GGCGCC GGC^GCC
SpeI 4165 ACTAGT A^CTAGT
SphI 1442 GCATGC GCATG^C
SspI 511 AATATT AAT^ATT
StuI 1981 AGGCCT AGG^CCT
XhoI 2594 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 4256 GTCTAC GT^[AC][GT]AC
5040 GTCTAC
AlwNI 3830 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
4015 CAGTCACTG
Asp700I 961 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2857 GAAGACCTTC
BcgI 2353 CGAGAACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2586 GCAACTTTCTCG
BsmI 2604 GAATGC GAATGC(1/-1)
2949 GAATGC
BsrDI 1098 CATTGC GCAATG(2/0)
2953 GCAATG
DraII 2828 AGGACCT [GA]G^G[ACGT]CC[CT]
3293 AGGACCT
EcoO109I 2828 AGGACCT [GA]G^G[ACGT]CC[CT]
3293 AGGACCT
HindIII 828 AAGCTT A^AGCTT
3874 AAGCTT
MspA1I 1562 CAGCTG C[AC]G^C[GT]G
2942 CAGCTG
NcoI 432 CCATGG C^CATGG
4149 CCATGG
NspI 1442 GCATGC [GA]CATG^[CT]
2741 GCATGT
PflMI 1891 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2508 CCAAACACTGG
PpuMI 2828 AGGACCT [GA]G^G[AT]CC[CT]
3293 AGGACCT
PstI 1319 CTGCAG CTGCA^G
4241 CTGCAG
PvuII 1562 CAGCTG CAG^CTG
2942 CAGCTG
Van91I 1891 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2508 CCAAACACTGG
XbaI 3028 TCTAGA T^CTAGA
4674 TCTAGA
XcmI 4751 CCAAACTTCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4805 CCAGGCCAGTCATGG
XmnI 961 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2857 GAAGACCTTC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AspHI
AvaII BbsI BbvI BciVI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaBI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PshBI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII