Restriction map for
pF1KSDA1083
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbrPI BbvCI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BspEI BspHI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MslI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfuI SmaI SnaBI
SphI Sse8387I SwaI Tth111I Van91I
XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1277 GTAGAC GT^[AC][GT]AC
Aor51HI 239 AGCGCT AGC^GCT
AviII 392 TGCGCA TGC^GCA
BalI 560 TGGCCA TGG^CCA
BcgI 292 GCACCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1531 GGATAC GTATCC(6/5)
BsaAI 1147 TACGTG [CT]AC^GT[GA]
BsmAI 1438 GAGAC GTCTC(1/5)
BsmI 1101 GAATGC GAATGC(1/-1)
Bsp1407I 1317 TGTACA T^GTACA
BspDI 397 ATCGAT AT^CGAT
BspMI 294 ACCTGC ACCTGC(4/8)
BsrGI 1317 TGTACA T^GTACA
BstF5I 405 GGATG GGATG(2/0)
ClaI 397 ATCGAT AT^CGAT
DraII 1578 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 722 CCATGG C^C[GA][CT]GG
EaeI 560 TGGCCA [CT]^GGCC[GA]
Eco47III 239 AGCGCT AGC^GCT
EcoNI 1024 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1578 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 3 GAATTC G^AATTC
FokI 405 GGATG GGATG(9/13)
FseI 110 GGCCGGCC GGCCGG^CC
FspI 392 TGCGCA TGC^GCA
MluNI 560 TGGCCA TGG^CCA
MscI 560 TGGCCA TGG^CCA
NaeI 111 GCCGGC GCC^GGC
NcoI 722 CCATGG C^CATGG
NgoMIV 111 GCCGGC G^CCGGC
PaeR7I 1193 CTCGAG C^TCGAG
PpuMI 1578 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 914 CAGCTG CAG^CTG
ScaI 1341 AGTACT AGT^ACT
SfiI 679 GGCCTTTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 317 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 1193 CTCGAG C^T[CT][GA]AG
SpeI 628 ACTAGT A^CTAGT
SspBI 1317 TGTACA T^GTACA
SspI 1639 AATATT AAT^ATT
Tsp45I 1544 GTGAC ^GT[GC]AC
XbaI 582 TCTAGA T^CTAGA
XhoI 1193 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AlwI 670 GGATC GGATC(4/5)
745 GGATC
AlwNI 290 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1085 CAGTAGCTG
AvaI 124 CCCGAG C^[CT]CG[GA]G
1193 CTCGAG
BbeI 43 GGCGCC GGCGC^C
315 GGCGCC
BbsI 1065 GAAGAC GAAGAC(2/6)
352 GTCTTC
BpmI 731 CTGGAG CTGGAG(16/14)
1328 CTGGAG
BpuAI 1065 GAAGAC GAAGAC(2/6)
352 GTCTTC
BsoBI 124 CCCGAG C^[CT]CG[GA]G
1193 CTCGAG
BsrDI 384 CATTGC GCAATG(2/0)
962 CATTGC
BsrFI 111 GCCGGC [GA]^CCGG[CT]
318 GCCGGT
BstYI 670 GGATCT [GA]^GATC[CT]
745 GGATCT
Cfr10I 111 GCCGGC [GA]^CCGG[CT]
318 GCCGGT
DpnII 671 GATC ^GATC
746 GATC
EarI 220 CTCTTC CTCTTC(1/4)
1253 GAAGAG
EcoT14I 563 CCAAGG C^C[AT][AT]GG
722 CCATGG
KasI 43 GGCGCC G^GCGCC
315 GGCGCC
Ksp632I 220 CTCTTC CTCTTC(1/4)
1253 GAAGAG
MaeII 1148 ACGT A^CGT
1719 ACGT
MboI 671 GATC ^GATC
746 GATC
MflI 670 GGATCT [GA]^GATC[CT]
745 GGATCT
MspA1I 166 CCGCTG C[AC]G^C[GT]G
914 CAGCTG
NarI 43 GGCGCC GG^CGCC
315 GGCGCC
NdeII 671 GATC ^GATC
746 GATC
NlaIII 258 CATG CATG^
723 CATG
PleI 127 GAGTC GAGTC(4/5)
350 GAGTC
PstI 674 CTGCAG CTGCA^G
1091 CTGCAG
SacI 273 GAGCTC GAGCT^C
1019 GAGCTC
Sau3AI 671 GATC ^GATC
746 GATC
SfcI 674 CTGCAG C^T[GA][CT]AG
1091 CTGCAG
SfoI 43 GGCGCC GGC^GCC
315 GGCGCC
StyI 563 CCAAGG C^C[AT][AT]GG
722 CCATGG
TfiI 1096 GAATC G^A[AT]TC
1663 GAATC
XhoII 670 GGATCT [GA]^GATC[CT]
745 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI AspHI AvaII BanI
BanII BbvI BcnI BfaI BglI
BmyI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstAPI BstNI BstUI Cac8I CfoI
Cfr13I DdeI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboII MnlI
MseI MspI MvaI MvnI MwoI
NciI NlaIV RsaI Sau96I ScrFI
SfaNI StuI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I