Restriction map for
pF1KSDA1088
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
ApoI AscI AseI AsnI Asp700I
AviII AvrII BbrPI BcgI BclI
BfrI BlnI BsaAI BseAI BsiWI
Bsp1407I BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I DraI
EcoRI EcoRV EcoT22I FbaI FspI
HincII HindII HindIII HpaI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI SalI ScaI SfiI SfuI
SnaBI SpeI SspBI SspI SwaI
Tru9I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 829 GACGTC GACGT^C
Acc65I 2045 GGTACC G^GTACC
AccI 31 GTAGAC GT^[AC][GT]AC
AflIII 4112 ACGTGT A^C[GA][CT]GT
AhdI 824 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2045 GGTACC G^GTACC
AspEI 824 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3006 GGATCC G^GATCC
BbvCI 2818 GCTGAGG CCTCAGC(-5/-2)
BciVI 2903 GTATCC GTATCC(6/5)
BfaI 3827 CTAG C^TAG
BsaBI 1473 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1292 ACCGGA [AT]^CCGG[AT]
BsmI 2948 GCATTC GAATGC(1/-1)
BspDI 985 ATCGAT AT^CGAT
BsrDI 942 CATTGC GCAATG(2/0)
BssSI 1393 CACGAG CACGAG(-5/-1)
ClaI 985 ATCGAT AT^CGAT
CpoI 3508 CGGTCCG CG^G[AT]CCG
Eam1105I 824 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 3409 GGCCGGCC GGCCGG^CC
KpnI 2045 GGTACC GGTAC^C
KspI 3218 CCGCGG CCGC^GG
MaeI 3827 CTAG C^TAG
MamI 1473 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PshAI 75 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 3508 CGGTCCG CG^G[AT]CCG
SacII 3218 CCGCGG CCGC^GG
SbfI 2754 CCTGCAGG CCTGCA^GG
SexAI 839 ACCAGGT A^CC[AT]GGT
SgrAI 1842 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1972 CTCAAG C^T[CT][GA]AG
SphI 1388 GCATGC GCATG^C
Sse8387I 2754 CCTGCAGG CCTGCA^GG
TfiI 781 GAATC G^A[AT]TC
Tsp509I 679 AATT ^AATT
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# TWO CUT ENZYMES ( 19 enzymes) #
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AspI 586 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3018 GACCGTGTC
BbeI 1846 GGCGCC GGCGC^C
3000 GGCGCC
BglII 1529 AGATCT A^GATCT
3539 AGATCT
BlpI 1303 GCTCAGC GC^T[ACGT]AGC
1356 GCTGAGC
Bpu1102I 1303 GCTCAGC GC^T[ACGT]AGC
1356 GCTGAGC
BsgI 3337 CTGCAC GTGCAG(16/14)
4079 GTGCAG
BstEII 1288 GGTCACC G^GT[ACGT]ACC
2414 GGTCACC
BstPI 1288 GGTCACC G^GT[ACGT]ACC
2414 GGTCACC
CelII 1303 GCTCAGC GC^T[ACGT]AGC
1356 GCTGAGC
DrdI 1012 GACAGCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2377 GACCTGCATGTC
EcoO65I 1288 GGTCACC G^GT[ACGT]ACC
2414 GGTCACC
KasI 1846 GGCGCC G^GCGCC
3000 GGCGCC
NarI 1846 GGCGCC GG^CGCC
3000 GGCGCC
PflFI 586 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3018 GACCGTGTC
SacI 1575 GAGCTC GAGCT^C
3695 GAGCTC
SapI 1701 GAAGAGC GCTCTTC(1/4)
2691 GCTCTTC
SfoI 1846 GGCGCC GGC^GCC
3000 GGCGCC
StuI 3821 AGGCCT AGG^CCT
3928 AGGCCT
Tth111I 586 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3018 GACCGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI