################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Aor51HI 629 AGCGCT AGC^GCT ApaI 1307 GGGCCC GGGCC^C AviII 1443 TGCGCA TGC^GCA BalI 920 TGGCCA TGG^CCA BglI 922 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1467 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 911 GGTCTC GGTCTC(1/5) BsmI 954 GAATGC GAATGC(1/-1) BsrBI 289 GAGCGG CCGCTC(-3/-3) BssSI 126 CTCGTG CACGAG(-5/-1) BstYI 1388 AGATCC [GA]^GATC[CT] Eco47III 629 AGCGCT AGC^GCT FspI 1443 TGCGCA TGC^GCA MaeII 164 ACGT A^CGT MaeIII 1154 GTCAC ^GT[ACGT]AC MamI 1467 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1388 AGATCC [GA]^GATC[CT] MluNI 920 TGGCCA TGG^CCA MscI 920 TGGCCA TGG^CCA MseI 1471 TTAA T^TAA NspI 1241 GCATGT [GA]CATG^[CT] PpuMI 659 GGGACCC [GA]G^G[AT]CC[CT] SfiI 921 GGCCACCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 27 CCCGGG CCC^GGG SpeI 276 ACTAGT A^CTAGT TaqI 494 TCGA T^CGA TfiI 843 GAATC G^A[AT]TC Tru9I 1471 TTAA T^TAA Tsp45I 1154 GTCAC ^GT[GC]AC TthHB8I 494 TCGA T^CGA XcmI 1088 CCACTGTATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1388 AGATCC [GA]^GATC[CT] XmaCI 27 CCCGGG C^CCGGG XmaI 27 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 344 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG 1269 CAGACCCTG Asp700I 309 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 505 GAAGTCCTTC AvaI 5 CCCGAG C^[CT]CG[GA]G 27 CCCGGG BanI 293 GGTGCC G^G[CT][GA]CC 751 GGCACC BanII 38 GGGCTC G[GA]GC[CT]^C 1307 GGGCCC BcnI 27 CCCGG CC^[GC]GG 28 CCGGG BfaI 277 CTAG C^TAG 901 CTAG BlpI 93 GCTGAGC GC^T[ACGT]AGC 795 GCTGAGC BpmI 440 CTGGAG CTGGAG(16/14) 1117 CTGGAG Bpu1102I 93 GCTGAGC GC^T[ACGT]AGC 795 GCTGAGC BseRI 762 CTCCTC GAGGAG(10/8) 1298 GAGGAG BsoBI 5 CCCGAG C^[CT]CG[GA]G 27 CCCGGG BspMI 548 ACCTGC ACCTGC(4/8) 1229 ACCTGC BstXI 297 CCAGACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 347 CCACTGGCATGG CelII 93 GCTGAGC GC^T[ACGT]AGC 795 GCTGAGC EaeI 920 TGGCCA [CT]^GGCC[GA] 1066 CGGCCA MaeI 277 CTAG C^TAG 901 CTAG NciI 27 CCCGG CC^[GC]GG 28 CCGGG NcoI 254 CCATGG C^CATGG 1379 CCATGG PleI 745 GACTC GAGTC(4/5) 976 GAGTC XmnI 309 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 505 GAAGTCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbsI BbvI BmyI BpuAI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI TspRI