Restriction map for pF1KSDA1094
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BsaAI       
BsaHI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfoI        
SfuI        SgrAI       SmlI        SnaBI       SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp509I     Tth111I     Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Aor51HI       629  AGCGCT               AGC^GCT
ApaI         1307  GGGCCC               GGGCC^C
AviII        1443  TGCGCA               TGC^GCA
BalI          920  TGGCCA               TGG^CCA
BglI          922  GCCACCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        1467  GATCTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          911  GGTCTC               GGTCTC(1/5)
BsmI          954  GAATGC               GAATGC(1/-1)
BsrBI         289  GAGCGG               CCGCTC(-3/-3)
BssSI         126  CTCGTG               CACGAG(-5/-1)
BstYI        1388  AGATCC               [GA]^GATC[CT]
Eco47III      629  AGCGCT               AGC^GCT
FspI         1443  TGCGCA               TGC^GCA
MaeII         164  ACGT                 A^CGT
MaeIII       1154  GTCAC                ^GT[ACGT]AC
MamI         1467  GATCTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1388  AGATCC               [GA]^GATC[CT]
MluNI         920  TGGCCA               TGG^CCA
MscI          920  TGGCCA               TGG^CCA
MseI         1471  TTAA                 T^TAA
NspI         1241  GCATGT               [GA]CATG^[CT]
PpuMI         659  GGGACCC              [GA]G^G[AT]CC[CT]
SfiI          921  GGCCACCTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI           27  CCCGGG               CCC^GGG
SpeI          276  ACTAGT               A^CTAGT
TaqI          494  TCGA                 T^CGA
TfiI          843  GAATC                G^A[AT]TC
Tru9I        1471  TTAA                 T^TAA
Tsp45I       1154  GTCAC                ^GT[GC]AC
TthHB8I       494  TCGA                 T^CGA
XcmI         1088  CCACTGTATGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1388  AGATCC               [GA]^GATC[CT]
XmaCI          27  CCCGGG               C^CCGGG
XmaI           27  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwNI         344  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1269  CAGACCCTG           
Asp700I       309  GAACCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              505  GAAGTCCTTC          
AvaI            5  CCCGAG               C^[CT]CG[GA]G
               27  CCCGGG              
BanI          293  GGTGCC               G^G[CT][GA]CC
              751  GGCACC              
BanII          38  GGGCTC               G[GA]GC[CT]^C
             1307  GGGCCC              
BcnI           27  CCCGG                CC^[GC]GG
               28  CCGGG               
BfaI          277  CTAG                 C^TAG
              901  CTAG                
BlpI           93  GCTGAGC              GC^T[ACGT]AGC
              795  GCTGAGC             
BpmI          440  CTGGAG               CTGGAG(16/14)
             1117  CTGGAG              
Bpu1102I       93  GCTGAGC              GC^T[ACGT]AGC
              795  GCTGAGC             
BseRI         762  CTCCTC               GAGGAG(10/8)
             1298  GAGGAG              
BsoBI           5  CCCGAG               C^[CT]CG[GA]G
               27  CCCGGG              
BspMI         548  ACCTGC               ACCTGC(4/8)
             1229  ACCTGC              
BstXI         297  CCAGACTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              347  CCACTGGCATGG        
CelII          93  GCTGAGC              GC^T[ACGT]AGC
              795  GCTGAGC             
EaeI          920  TGGCCA               [CT]^GGCC[GA]
             1066  CGGCCA              
MaeI          277  CTAG                 C^TAG
              901  CTAG                
NciI           27  CCCGG                CC^[GC]GG
               28  CCGGG               
NcoI          254  CCATGG               C^CATGG
             1379  CCATGG              
PleI          745  GACTC                GAGTC(4/5)
              976  GAGTC               
XmnI          309  GAACCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              505  GAAGTCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbsI        BbvI        BmyI        BpuAI       BsaJI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspHI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TseI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com