Restriction map for
pF1KSDA1099
##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AclI AflII AocI AscI AseI
AsnI AspI AvrII BbeI BbrPI
BbvCI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsiWI Bsp1407I
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII CpoI DraI Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MunI
NaeI NarI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 2221 GACGTC GACGT^C
Acc65I 269 GGTACC G^GTACC
AccI 1942 GTAGAC GT^[AC][GT]AC
AcsI 1497 AAATTT [GA]^AATT[CT]
AflIII 628 ACGTGT A^C[GA][CT]GT
AgeI 266 ACCGGT A^CCGGT
ApaI 378 GGGCCC GGGCC^C
ApoI 1497 AAATTT [GA]^AATT[CT]
Asp700I 1995 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 269 GGTACC G^GTACC
AviII 1249 TGCGCA TGC^GCA
BcgI 624 CGAGACGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 362 TGATCA T^GATCA
BsaAI 615 CACGTA [CT]AC^GT[GA]
BsgI 2019 CTGCAC GTGCAG(16/14)
BsmI 1777 GAATGC GAATGC(1/-1)
BssSI 501 CACGAG CACGAG(-5/-1)
BstXI 401 CCATGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1570 GACGCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1281 CCTATCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 362 TGATCA T^GATCA
FspI 1249 TGCGCA TGC^GCA
KpnI 269 GGTACC GGTAC^C
NcoI 1667 CCATGG C^CATGG
NdeI 284 CATATG CA^TATG
NheI 1890 GCTAGC G^CTAGC
PinAI 266 ACCGGT A^CCGGT
SapI 1998 GCTCTTC GCTCTTC(1/4)
SmaI 2108 CCCGGG CCC^GGG
SphI 1052 GCATGC GCATG^C
XmaCI 2108 CCCGGG C^CCGGG
XmaI 2108 CCCGGG C^CCGGG
XmnI 1995 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 303 TCCGGA T^CCGGA
1685 TCCGGA
AhdI 189 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1462 GACGGCCTGTC
Aor51HI 53 AGCGCT AGC^GCT
2327 AGCGCT
AspEI 189 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1462 GACGGCCTGTC
BamHI 1787 GGATCC G^GATCC
1973 GGATCC
BbsI 427 GTCTTC GAAGAC(2/6)
661 GTCTTC
BfaI 1445 CTAG C^TAG
1891 CTAG
BpuAI 427 GTCTTC GAAGAC(2/6)
661 GTCTTC
BsaI 1723 GAGACC GGTCTC(1/5)
2125 GAGACC
BseAI 303 TCCGGA T^CCGGA
1685 TCCGGA
BsiEI 1026 CGGCCG CG[GA][CT]^CG
2145 CGGCCG
BsmBI 625 GAGACG CGTCTC(1/5)
2141 GAGACG
BspDI 568 ATCGAT AT^CGAT
883 ATCGAT
BspEI 303 TCCGGA T^CCGGA
1685 TCCGGA
BsrBI 1023 GAGCGG CCGCTC(-3/-3)
1564 GAGCGG
BstAPI 1772 GCATCGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2043 GCACCTGCTGC
ClaI 568 ATCGAT AT^CGAT
883 ATCGAT
DraIII 1110 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1708 CACTGTGTG
DsaI 1667 CCATGG C^C[GA][CT]GG
2241 CCACGG
EagI 1026 CGGCCG C^GGCCG
2145 CGGCCG
Eam1105I 189 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1462 GACGGCCTGTC
EclXI 1026 CGGCCG C^GGCCG
2145 CGGCCG
Eco47III 53 AGCGCT AGC^GCT
2327 AGCGCT
Eco52I 1026 CGGCCG C^GGCCG
2145 CGGCCG
EcoT14I 1286 CCAAGG C^C[AT][AT]GG
1667 CCATGG
MaeI 1445 CTAG C^TAG
1891 CTAG
MroI 303 TCCGGA T^CCGGA
1685 TCCGGA
MseI 823 TTAA T^TAA
1043 TTAA
PflMI 1596 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1707 CCACTGTGTGG
PpuMI 720 AGGACCC [GA]G^G[AT]CC[CT]
2069 AGGACCT
PvuII 16 CAGCTG CAG^CTG
709 CAGCTG
SacI 211 GAGCTC GAGCT^C
1855 GAGCTC
StuI 993 AGGCCT AGG^CCT
2273 AGGCCT
StyI 1286 CCAAGG C^C[AT][AT]GG
1667 CCATGG
Tru9I 823 TTAA T^TAA
1043 TTAA
Van91I 1596 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1707 CCACTGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII