Restriction map for
pF1KSDA1112
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# NO CUT ENZYMES ( 101 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp718I AspEI
AviII BamHI BbrPI BciVI BclI
BfrI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I MfeI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 655 GACGTC GACGT^C
AlwI 873 GATCC GGATC(4/5)
Aor51HI 1784 AGCGCT AGC^GCT
ApaI 110 GGGCCC GGGCC^C
AspI 610 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 163 CCTAGG C^CTAGG
BalI 403 TGGCCA TGG^CCA
BbeI 1732 GGCGCC GGCGC^C
BbsI 216 GAAGAC GAAGAC(2/6)
BglI 784 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 163 CCTAGG C^CTAGG
BlpI 1182 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1182 GCTCAGC GC^T[ACGT]AGC
BpuAI 216 GAAGAC GAAGAC(2/6)
BsaAI 1192 TACGTG [CT]AC^GT[GA]
BsaBI 1233 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1688 GAGACC GGTCTC(1/5)
BsaWI 1266 TCCGGT [AT]^CCGG[AT]
BsmI 1884 GAATGC GAATGC(1/-1)
BspLU11I 956 ACATGT A^CATGT
BssHII 948 GCGCGC G^CGCGC
BstAPI 1872 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 92 CCACTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1182 GCTCAGC GC^T[ACGT]AGC
DrdI 448 GACACAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1784 AGCGCT AGC^GCT
EcoNI 1799 CCTTTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 781 GACGC GACGC(5/10)
KasI 1732 GGCGCC G^GCGCC
KspI 770 CCGCGG CCGC^GG
MamI 1233 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 403 TGGCCA TGG^CCA
MscI 403 TGGCCA TGG^CCA
NarI 1732 GGCGCC GG^CGCC
NcoI 620 CCATGG C^CATGG
PaeR7I 1520 CTCGAG C^TCGAG
PflFI 610 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 630 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 855 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 770 CCGCGG CCGC^GG
ScaI 1130 AGTACT AGT^ACT
SfoI 1732 GGCGCC GGC^GCC
SphI 1365 GCATGC GCATG^C
Tth111I 610 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 630 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1520 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
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Alw44I 280 GTGCAC G^TGCAC
1758 GTGCAC
ApaLI 280 GTGCAC G^TGCAC
1758 GTGCAC
BanI 1084 GGTGCC G^G[CT][GA]CC
1732 GGCGCC
BbvCI 876 CCTCAGC CCTCAGC(-5/-2)
747 GCTGAGG
BcgI 1077 CGAGCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1425 CGACAAGATTGC
BfaI 164 CTAG C^TAG
347 CTAG
BsrFI 1214 GCCGGC [GA]^CCGG[CT]
1634 GCCGGC
BsrI 1460 ACTGG ACTGG(1/-1)
32 CCAGT
Cfr10I 1214 GCCGGC [GA]^CCGG[CT]
1634 GCCGGC
EcoT14I 163 CCTAGG C^C[AT][AT]GG
620 CCATGG
MaeI 164 CTAG C^TAG
347 CTAG
NaeI 1214 GCCGGC GCC^GGC
1634 GCCGGC
NgoMIV 1214 GCCGGC G^CCGGC
1634 GCCGGC
NspI 956 ACATGT [GA]CATG^[CT]
1365 GCATGC
PpuMI 485 AGGACCT [GA]G^G[AT]CC[CT]
1598 AGGACCT
StyI 163 CCTAGG C^C[AT][AT]GG
620 CCATGG
TfiI 339 GAATC G^A[AT]TC
756 GAATC
XcmI 1148 CCAACGCCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1370 CCATGAAGAACGTGG
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI Asp700I AspHI AvaI
AvaII BanII BbvI BcnI BglII
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI TaqI TseI Tsp45I TspRI
TthHB8I XhoII XmnI