Restriction map for
pF1KSDA1129
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII BbeI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BpuAI BsaI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HpaI KasI KpnI
KspI MluI MroI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 1122 GACGTC GACGT^C
AccII 1286 CGCG CG^CG
AcsI 559 GAATTT [GA]^AATT[CT]
AcyI 1122 GACGTC G[GA]^CG[CT]C
AgeI 974 ACCGGT A^CCGGT
ApoI 559 GAATTT [GA]^AATT[CT]
AvrII 733 CCTAGG C^CTAGG
BalI 1038 TGGCCA TGG^CCA
BamHI 1639 GGATCC G^GATCC
BanI 927 GGCACC G^G[CT][GA]CC
BbrPI 1042 CACGTG CAC^GTG
BcnI 372 CCCGG CC^[GC]GG
BglI 1436 GCCGCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 733 CCTAGG C^CTAGG
BpmI 884 CTCCAG CTGGAG(16/14)
BsaBI 270 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1122 GACGTC G[GA]^CG[CT]C
BsaWI 974 ACCGGT [AT]^CCGG[AT]
BsmFI 737 GGGAC GGGAC(10/14)
BsmI 106 GCATTC GAATGC(1/-1)
Bsp1407I 480 TGTACA T^GTACA
BspHI 905 TCATGA T^CATGA
BsrDI 1128 CATTGC GCAATG(2/0)
BsrGI 480 TGTACA T^GTACA
BstAPI 1732 GCAGGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1286 CGCG CG^CG
BstXI 449 CCAATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1639 GGATCC [GA]^GATC[CT]
DraIII 190 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 739 GACTACCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1052 CCGTGG C^C[GA][CT]GG
EcoRV 597 GATATC GAT^ATC
HaeII 1145 AGCGCC [GA]GCGC^[CT]
Hin1I 1122 GACGTC G[GA]^CG[CT]C
HincII 82 GTCAAC GT[CT]^[GA]AC
HindII 82 GTCAAC GT[CT]^[GA]AC
HindIII 767 AAGCTT A^AGCTT
MamI 270 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 450 CAATTG C^AATTG
MflI 1639 GGATCC [GA]^GATC[CT]
MluNI 1038 TGGCCA TGG^CCA
MscI 1038 TGGCCA TGG^CCA
MunI 450 CAATTG C^AATTG
MvnI 1286 CGCG CG^CG
NaeI 1577 GCCGGC GCC^GGC
NciI 372 CCCGG CC^[GC]GG
NgoMIV 1577 GCCGGC G^CCGGC
PinAI 974 ACCGGT A^CCGGT
PmaCI 1042 CACGTG CAC^GTG
PmlI 1042 CACGTG CAC^GTG
PshAI 882 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 905 TCATGA T^CATGA
SacI 24 GAGCTC GAGCT^C
SapI 1512 GAAGAGC GCTCTTC(1/4)
SexAI 581 ACCAGGT A^CC[AT]GGT
SphI 1136 GCATGC GCATG^C
SspBI 480 TGTACA T^GTACA
TfiI 1746 GATTC G^A[AT]TC
XcmI 446 CCACCAATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1639 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Asp700I 102 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
556 GAAGAATTTC
BanII 24 GAGCTC G[GA]GC[CT]^C
1609 GGGCTC
BsaAI 1042 CACGTG [CT]AC^GT[GA]
1414 TACGTG
BseRI 829 CTCCTC GAGGAG(10/8)
967 GAGGAG
BsmAI 846 GAGAC GTCTC(1/5)
432 GTCTC
BsrFI 974 ACCGGT [GA]^CCGG[CT]
1577 GCCGGC
Cfr10I 974 ACCGGT [GA]^CCGG[CT]
1577 GCCGGC
EaeI 921 CGGCCA [CT]^GGCC[GA]
1038 TGGCCA
EcoNI 804 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
894 CCTTCCCAAGG
HgaI 297 GACGC GACGC(5/10)
1692 GACGC
MseI 857 TTAA T^TAA
1064 TTAA
PvuII 139 CAGCTG CAG^CTG
1471 CAGCTG
ScaI 1213 AGTACT AGT^ACT
1664 AGTACT
Tru9I 857 TTAA T^TAA
1064 TTAA
XmnI 102 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
556 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaII BbvI BfaI
BlpI BmyI Bpu1102I BsaJI BsgI
BsiHKAI BsiYI BslI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CelII CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I