Restriction map for
pF1KSDA1142
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AvrII BamHI BbrPI BbsI BbvCI
BcgI BclI BfaI BfrI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tru9I Tsp509I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AfaI 684 GTAC GT^AC
AhdI 1030 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 61 GTGCAC G^TGCAC
AlwI 876 GATCC GGATC(4/5)
ApaLI 61 GTGCAC G^TGCAC
Asp700I 1176 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1030 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 754 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 742 GGCGCC GGCGC^C
BciVI 436 GTATCC GTATCC(6/5)
BglI 1213 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 29 AGATCT A^GATCT
BsaI 1205 GAGACC GGTCTC(1/5)
BsiEI 794 CGGCCG CG[GA][CT]^CG
BsmAI 1205 GAGAC GTCTC(1/5)
Bsp1407I 683 TGTACA T^GTACA
BsrFI 143 GCCGGC [GA]^CCGG[CT]
BsrGI 683 TGTACA T^GTACA
BstYI 29 AGATCT [GA]^GATC[CT]
Cfr10I 143 GCCGGC [GA]^CCGG[CT]
DdeI 339 CTCAG C^T[ACGT]AG
EagI 794 CGGCCG C^GGCCG
Eam1105I 1030 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 794 CGGCCG C^GGCCG
Eco52I 794 CGGCCG C^GGCCG
HaeII 742 GGCGCC [GA]GCGC^[CT]
HgaI 845 GCGTC GACGC(5/10)
HincII 160 GTCGAC GT[CT]^[GA]AC
HindII 160 GTCGAC GT[CT]^[GA]AC
KasI 742 GGCGCC G^GCGCC
MaeII 218 ACGT A^CGT
MaeIII 760 GTCAC ^GT[ACGT]AC
MflI 29 AGATCT [GA]^GATC[CT]
NaeI 143 GCCGGC GCC^GGC
NarI 742 GGCGCC GG^CGCC
NgoMIV 143 GCCGGC G^CCGGC
PflFI 754 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 327 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 684 GTAC GT^AC
SalI 160 GTCGAC G^TCGAC
SapI 627 GCTCTTC GCTCTTC(1/4)
SexAI 695 ACCTGGT A^CC[AT]GGT
SfoI 742 GGCGCC GGC^GCC
SmaI 190 CCCGGG CCC^GGG
SspBI 683 TGTACA T^GTACA
TfiI 1119 GATTC G^A[AT]TC
Tsp45I 760 GTCAC ^GT[GC]AC
Tth111I 754 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 29 AGATCT [GA]^GATC[CT]
XmaCI 190 CCCGGG C^CCGGG
XmaI 190 CCCGGG C^CCGGG
XmnI 1176 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 160 GTCGAC GT^[AC][GT]AC
1027 GTAGAC
AcyI 742 GGCGCC G[GA]^CG[CT]C
844 GGCGTC
AlwNI 118 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
817 CAGGCCCTG
ApaI 194 GGGCCC GGGCC^C
394 GGGCCC
AviII 560 TGCGCA TGC^GCA
602 TGCGCA
BalI 251 TGGCCA TGG^CCA
1256 TGGCCA
BanI 304 GGTGCC G^G[CT][GA]CC
742 GGCGCC
BpmI 359 CTCCAG CTGGAG(16/14)
317 CTGGAG
BsaHI 742 GGCGCC G[GA]^CG[CT]C
844 GGCGTC
BsgI 366 CTGCAC GTGCAG(16/14)
1153 CTGCAC
BspHI 213 TCATGA T^CATGA
647 TCATGA
BsrBI 408 CCGCTC CCGCTC(-3/-3)
492 CCGCTC
BssSI 82 CACGAG CACGAG(-5/-1)
664 CACGAG
BstAPI 54 GCACCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
539 GCATCGTGTGC
DraIII 55 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1156 CACAAGGTG
DsaI 533 CCACGG C^C[GA][CT]GG
1223 CCACGG
EarI 628 CTCTTC CTCTTC(1/4)
9 GAAGAG
EcoT14I 200 CCAAGG C^C[AT][AT]GG
1259 CCAAGG
FspI 560 TGCGCA TGC^GCA
602 TGCGCA
Hin1I 742 GGCGCC G[GA]^CG[CT]C
844 GGCGTC
Ksp632I 628 CTCTTC CTCTTC(1/4)
9 GAAGAG
MluNI 251 TGGCCA TGG^CCA
1256 TGGCCA
MscI 251 TGGCCA TGG^CCA
1256 TGGCCA
PleI 871 GACTC GAGTC(4/5)
133 GAGTC
PstI 463 CTGCAG CTGCA^G
814 CTGCAG
PvuII 349 CAGCTG CAG^CTG
466 CAGCTG
RcaI 213 TCATGA T^CATGA
647 TCATGA
SacI 709 GAGCTC GAGCT^C
994 GAGCTC
SfcI 463 CTGCAG C^T[GA][CT]AG
814 CTGCAG
StyI 200 CCAAGG C^C[AT][AT]GG
1259 CCAAGG
TspRI 112 CAGTG CA[GC]TG[ACGT][ACGT]^
809 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII DraII EaeI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TthHB8I