Restriction map for
pF1KSDA1169
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
Asp718I AspEI AspI AviII AvrII
BanI BbeI BciVI BfrI BlnI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoRI EcoT22I FseI FspI HindIII
HpaI KasI KpnI KspI MfeI
MluI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PpuMI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 1513 TCCGGA T^CCGGA
ApaI 1491 GGGCCC GGGCC^C
AseI 1300 ATTAAT AT^TAAT
AsnI 1300 ATTAAT AT^TAAT
Asp700I 1404 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1370 GGATCC G^GATCC
BbrPI 1420 CACGTG CAC^GTG
BbvCI 1629 GCTGAGG CCTCAGC(-5/-2)
BcgI 1209 CGAAGTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 728 TGATCA T^GATCA
BfaI 104 CTAG C^TAG
BglI 61 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 671 AGATCT A^GATCT
BlpI 1179 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1179 GCTCAGC GC^T[ACGT]AGC
BsaAI 1420 CACGTG [CT]AC^GT[GA]
BsaBI 2034 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2224 GAGACC GGTCTC(1/5)
BsaWI 1513 TCCGGA [AT]^CCGG[AT]
BseAI 1513 TCCGGA T^CCGGA
BspEI 1513 TCCGGA T^CCGGA
BsrBI 28 CCGCTC CCGCTC(-3/-3)
BstEII 597 GGTGACC G^GT[ACGT]ACC
BstPI 597 GGTGACC G^GT[ACGT]ACC
CelII 1179 GCTCAGC GC^T[ACGT]AGC
DraIII 1834 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 597 GGTGACC G^GT[ACGT]ACC
EcoRV 1201 GATATC GAT^ATC
FbaI 728 TGATCA T^GATCA
MaeI 104 CTAG C^TAG
MamI 2034 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1513 TCCGGA T^CCGGA
PaeR7I 2059 CTCGAG C^TCGAG
PflMI 1833 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1420 CACGTG CAC^GTG
PmlI 1420 CACGTG CAC^GTG
PshAI 516 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1300 ATTAAT AT^TAAT
PvuII 1624 CAGCTG CAG^CTG
ScaI 1502 AGTACT AGT^ACT
TfiI 1158 GAATC G^A[AT]TC
Van91I 1833 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2059 CTCGAG C^TCGAG
XmnI 1404 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 649 GGCGTC G[GA]^CG[CT]C
1963 GGCGTC
AlwNI 679 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1624 CAGCTGCTG
AvaI 602 CCCGAG C^[CT]CG[GA]G
2059 CTCGAG
BalI 338 TGGCCA TGG^CCA
1700 TGGCCA
BsaHI 649 GGCGTC G[GA]^CG[CT]C
1963 GGCGTC
BsoBI 602 CCCGAG C^[CT]CG[GA]G
2059 CTCGAG
BspMI 662 ACCTGC ACCTGC(4/8)
938 ACCTGC
BsrFI 1066 GCCGGC [GA]^CCGG[CT]
1483 GCCGGC
BssSI 1797 CACGAG CACGAG(-5/-1)
1375 CTCGTG
Cfr10I 1066 GCCGGC [GA]^CCGG[CT]
1483 GCCGGC
EaeI 338 TGGCCA [CT]^GGCC[GA]
1700 TGGCCA
EcoNI 369 CCTGTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2130 CCTTGAGAAGG
Hin1I 649 GGCGTC G[GA]^CG[CT]C
1963 GGCGTC
HincII 1357 GTCAAC GT[CT]^[GA]AC
1936 GTCAAC
HindII 1357 GTCAAC GT[CT]^[GA]AC
1936 GTCAAC
MluNI 338 TGGCCA TGG^CCA
1700 TGGCCA
MscI 338 TGGCCA TGG^CCA
1700 TGGCCA
MseI 1301 TTAA T^TAA
1640 TTAA
MspA1I 1624 CAGCTG C[AC]G^C[GT]G
1821 CCGCTG
NaeI 1066 GCCGGC GCC^GGC
1483 GCCGGC
NgoMIV 1066 GCCGGC G^CCGGC
1483 GCCGGC
NspI 1029 GCATGC [GA]CATG^[CT]
2349 GCATGC
PleI 136 GACTC GAGTC(4/5)
2104 GACTC
SacI 1924 GAGCTC GAGCT^C
2173 GAGCTC
SapI 94 GAAGAGC GCTCTTC(1/4)
2152 GAAGAGC
SfcI 1077 CTACAG C^T[GA][CT]AG
2085 CTACAG
SmlI 2059 CTCGAG C^T[CT][GA]AG
2131 CTTGAG
SphI 1029 GCATGC GCATG^C
2349 GCATGC
Tru9I 1301 TTAA T^TAA
1640 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AfaI AluI AlwI Aor51HI
AspHI AvaII BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII