Restriction map for
pF1KSDA1171
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I ApaLI
ApoI AseI AsnI Asp718I AspHI
AvrII BamHI BbeI BbrPI BbvCI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaI BseAI BsiHKAI BsiWI
BsmBI BspEI BspHI BspLU11I BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DraI DraIII DrdI EcoO65I
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MunI NarI NcoI
NdeI NheI NotI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SfoI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
TfiI Tru9I Tsp509I XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 688 GACGTC GACGT^C
AhdI 1054 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1309 CCTGAGG CC^T[ACGT]AGG
AscI 114 GGCGCGCC GG^CGCGCC
Asp700I 861 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1054 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 341 TGCGCA TGC^GCA
BanI 300 GGTGCC G^G[CT][GA]CC
BcgI 1074 CGAGCGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 13 GGATAC GTATCC(6/5)
BclI 1337 TGATCA T^GATCA
BlpI 78 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 78 GCTGAGC GC^T[ACGT]AGC
BsaWI 1279 ACCGGA [AT]^CCGG[AT]
BsiEI 404 CGGCCG CG[GA][CT]^CG
BspDI 1552 ATCGAT AT^CGAT
BsrFI 402 GCCGGC [GA]^CCGG[CT]
BssHII 115 GCGCGC G^CGCGC
BstXI 1487 CCATGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1309 CCTGAGG CC^T[ACGT]AGG
CelII 78 GCTGAGC GC^T[ACGT]AGC
Cfr10I 402 GCCGGC [GA]^CCGG[CT]
ClaI 1552 ATCGAT AT^CGAT
EagI 404 CGGCCG C^GGCCG
Eam1105I 1054 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 960 GAAGAG CTCTTC(1/4)
EclXI 404 CGGCCG C^GGCCG
Eco52I 404 CGGCCG C^GGCCG
Eco81I 1309 CCTGAGG CC^T[ACGT]AGG
EcoNI 696 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 71 CCAAGG C^C[AT][AT]GG
FbaI 1337 TGATCA T^GATCA
FspI 341 TGCGCA TGC^GCA
Ksp632I 960 GAAGAG CTCTTC(1/4)
NaeI 402 GCCGGC GCC^GGC
NgoMIV 402 GCCGGC G^CCGGC
NruI 827 TCGCGA TCG^CGA
SbfI 615 CCTGCAGG CCTGCA^GG
ScaI 566 AGTACT AGT^ACT
SexAI 554 ACCTGGT A^CC[AT]GGT
SfiI 405 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 677 CCCGGG CCC^GGG
SmlI 742 CTCAAG C^T[CT][GA]AG
Sse8387I 615 CCTGCAGG CCTGCA^GG
StyI 71 CCAAGG C^C[AT][AT]GG
XcmI 53 CCATCCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 677 CCCGGG C^CCGGG
XmaI 677 CCCGGG C^CCGGG
XmnI 861 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 208 GACGCC G[GA]^CG[CT]C
688 GACGTC
ApaI 67 GGGCCC GGGCC^C
131 GGGCCC
AspI 834 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1479 GACCGAGTC
BbsI 831 GAAGAC GAAGAC(2/6)
691 GTCTTC
BpuAI 831 GAAGAC GAAGAC(2/6)
691 GTCTTC
BsaHI 208 GACGCC G[GA]^CG[CT]C
688 GACGTC
BsmAI 821 GAGAC GTCTC(1/5)
1052 GAGAC
BsmI 318 GAATGC GAATGC(1/-1)
1285 GAATGC
Bsp1407I 218 TGTACA T^GTACA
990 TGTACA
BsrBI 1020 CCGCTC CCGCTC(-3/-3)
1451 CCGCTC
BsrDI 1632 CATTGC GCAATG(2/0)
482 GCAATG
BsrGI 218 TGTACA T^GTACA
990 TGTACA
BstAPI 257 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1505 GCAGCGACTGC
BstYI 347 AGATCC [GA]^GATC[CT]
896 AGATCC
DsaI 407 CCGTGG C^C[GA][CT]GG
1640 CCGTGG
HgaI 46 GACGC GACGC(5/10)
208 GACGC
Hin1I 208 GACGCC G[GA]^CG[CT]C
688 GACGTC
MaeIII 199 GTCAC ^GT[ACGT]AC
969 GTCAC
MflI 347 AGATCC [GA]^GATC[CT]
896 AGATCC
PflFI 834 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1479 GACCGAGTC
PflMI 57 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1226 CCACAGACTGG
PvuII 109 CAGCTG CAG^CTG
261 CAGCTG
SspBI 218 TGTACA T^GTACA
990 TGTACA
StuI 461 AGGCCT AGG^CCT
1646 AGGCCT
Tsp45I 199 GTCAC ^GT[GC]AC
969 GTCAC
Tth111I 834 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1479 GACCGAGTC
Van91I 57 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1226 CCACAGACTGG
XhoII 347 AGATCC [GA]^GATC[CT]
896 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Aor51HI AvaI AvaII BalI
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI Eco47III EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI TspRI
TthHB8I