Restriction map for
pF1KSDA1184
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
AspEI AspI AvrII BamHI BbeI
BbsI BciVI BclI BfrI BglII
BlnI BpuAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BsrBI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
Hin1I HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 266 GGTACC G^GTACC
AccI 703 GTCTAC GT^[AC][GT]AC
AccII 55 CGCG CG^CG
AlwI 660 GATCC GGATC(4/5)
AocI 589 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1007 AGCGCT AGC^GCT
Asp700I 181 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 266 GGTACC G^GTACC
AviII 290 TGCGCA TGC^GCA
BalI 681 TGGCCA TGG^CCA
BbrPI 981 CACGTG CAC^GTG
BbvCI 549 CCTCAGC CCTCAGC(-5/-2)
BcgI 254 GCACCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 841 CTAG C^TAG
BglI 418 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 813 GCTGAGC GC^T[ACGT]AGC
BpmI 967 CTGGAG CTGGAG(16/14)
Bpu1102I 813 GCTGAGC GC^T[ACGT]AGC
BsaAI 981 CACGTG [CT]AC^GT[GA]
BsaI 805 GAGACC GGTCTC(1/5)
BsmAI 805 GAGAC GTCTC(1/5)
Bsp1407I 245 TGTACA T^GTACA
BspHI 870 TCATGA T^CATGA
BsrDI 797 GCAATG GCAATG(2/0)
BsrGI 245 TGTACA T^GTACA
BstAPI 945 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 486 GGTCACC G^GT[ACGT]ACC
BstPI 486 GGTCACC G^GT[ACGT]ACC
BstUI 55 CGCG CG^CG
BstYI 659 AGATCC [GA]^GATC[CT]
Bsu36I 589 CCTCAGG CC^T[ACGT]AGG
CelII 813 GCTGAGC GC^T[ACGT]AGC
DpnII 660 GATC ^GATC
EaeI 681 TGGCCA [CT]^GGCC[GA]
Eco47III 1007 AGCGCT AGC^GCT
Eco81I 589 CCTCAGG CC^T[ACGT]AGG
EcoNI 996 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 486 GGTCACC G^GT[ACGT]ACC
EcoT14I 491 CCTTGG C^C[AT][AT]GG
FspI 290 TGCGCA TGC^GCA
HgaI 56 GCGTC GACGC(5/10)
KpnI 266 GGTACC GGTAC^C
MaeI 841 CTAG C^TAG
MaeII 982 ACGT A^CGT
MboI 660 GATC ^GATC
MflI 659 AGATCC [GA]^GATC[CT]
MluNI 681 TGGCCA TGG^CCA
MscI 681 TGGCCA TGG^CCA
MvnI 55 CGCG CG^CG
NdeII 660 GATC ^GATC
PmaCI 981 CACGTG CAC^GTG
PmlI 981 CACGTG CAC^GTG
RcaI 870 TCATGA T^CATGA
Sau3AI 660 GATC ^GATC
SspBI 245 TGTACA T^GTACA
StyI 491 CCTTGG C^C[AT][AT]GG
XcmI 212 CCATGAAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 659 AGATCC [GA]^GATC[CT]
XmnI 181 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspHI 816 GAGCTC G[AT]GC[AT]^C
1120 GAGCTC
BanI 266 GGTACC G^G[CT][GA]CC
560 GGCACC
BseRI 883 GAGGAG GAGGAG(10/8)
1003 GAGGAG
BsgI 460 GTGCAG GTGCAG(16/14)
952 GTGCAG
BsiHKAI 816 GAGCTC G[AT]GC[AT]^C
1120 GAGCTC
BspMI 758 ACCTGC ACCTGC(4/8)
901 GCAGGT
BsrFI 554 GCCGGC [GA]^CCGG[CT]
1127 GCCGGC
Cfr10I 554 GCCGGC [GA]^CCGG[CT]
1127 GCCGGC
HinfI 69 GATTC G^A[ACGT]TC
303 GAATC
HphI 488 TCACC GGTGA(8/7)
611 TCACC
MaeIII 487 GTCAC ^GT[ACGT]AC
545 GTGAC
MslI 628 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
871 CATGAGTATG
NaeI 554 GCCGGC GCC^GGC
1127 GCCGGC
NgoMIV 554 GCCGGC G^CCGGC
1127 GCCGGC
PflMI 1035 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1107 CCAGCTCCTGG
PvuII 286 CAGCTG CAG^CTG
421 CAGCTG
SacI 816 GAGCTC GAGCT^C
1120 GAGCTC
SapI 1117 GAAGAGC GCTCTTC(1/4)
762 GCTCTTC
SfaNI 942 GATGC GCATC(5/9)
599 GCATC
SfcI 390 CTACAG C^T[GA][CT]AG
1048 CTACAG
TaqI 325 TCGA T^CGA
407 TCGA
TfiI 69 GATTC G^A[AT]TC
303 GAATC
Tsp45I 487 GTCAC ^GT[GC]AC
545 GTGAC
TthHB8I 325 TCGA T^CGA
407 TCGA
Van91I 1035 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1107 CCAGCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AvaI AvaII BanII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI Ksp632I
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PpuMI
RsaI Sau96I ScrFI SmaI TseI
TspRI XmaCI XmaI