Restriction map for
pF1KSDA1194
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BciVI
BclI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
CelII Cfr10I CpoI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I FbaI FseI
FspI HgaI Hin1I HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 42 GTATAC GT^[AC][GT]AC
AflII 126 CTTAAG C^TTAAG
AflIII 834 ACATGT A^C[GA][CT]GT
AlwI 1382 GGATC GGATC(4/5)
AocI 1505 CCTTAGG CC^T[ACGT]AGG
ApaI 1518 GGGCCC GGGCC^C
Asp700I 194 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 810 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1492 CCTCAGC CCTCAGC(-5/-2)
BcgI 1013 CGATTGAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 608 CCCGG CC^[GC]GG
BfrI 126 CTTAAG C^TTAAG
BglII 1233 AGATCT A^GATCT
BpmI 1612 CTGGAG CTGGAG(16/14)
BsgI 1229 GTGCAG GTGCAG(16/14)
BsmAI 1334 GTCTC GTCTC(1/5)
BspDI 1011 ATCGAT AT^CGAT
BspHI 959 TCATGA T^CATGA
BspLU11I 834 ACATGT A^CATGT
Bst1107I 42 GTATAC GTA^TAC
BstYI 1233 AGATCT [GA]^GATC[CT]
BstZ17I 42 GTATAC GTA^TAC
Bsu36I 1505 CCTTAGG CC^T[ACGT]AGG
ClaI 1011 ATCGAT AT^CGAT
DraI 309 TTTAAA TTT^AAA
DsaI 917 CCATGG C^C[GA][CT]GG
Eco81I 1505 CCTTAGG CC^T[ACGT]AGG
EcoRI 194 GAATTC G^AATTC
EcoRV 700 GATATC GAT^ATC
EcoT22I 1084 ATGCAT ATGCA^T
HaeII 639 GGCGCT [GA]GCGC^[CT]
HapII 609 CCGG C^CGG
HincII 1642 GTCAAC GT[CT]^[GA]AC
HindII 1642 GTCAAC GT[CT]^[GA]AC
HpaII 609 CCGG C^CGG
HphI 153 TCACC GGTGA(8/7)
MaeII 768 ACGT A^CGT
MflI 1233 AGATCT [GA]^GATC[CT]
MspI 609 CCGG C^CGG
NciI 608 CCCGG CC^[GC]GG
NcoI 917 CCATGG C^CATGG
NheI 912 GCTAGC G^CTAGC
NsiI 1084 ATGCAT ATGCA^T
PflFI 810 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1091 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 959 TCATGA T^CATGA
ScaI 987 AGTACT AGT^ACT
SfcI 654 CTACAG C^T[GA][CT]AG
StuI 375 AGGCCT AGG^CCT
SwaI 308 ATTTAAAT ATTT^AAAT
TaqI 1012 TCGA T^CGA
Tth111I 810 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1012 TCGA T^CGA
XbaI 799 TCTAGA T^CTAGA
XhoII 1233 AGATCT [GA]^GATC[CT]
XmnI 194 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 269 CCGC CCGC(-3/-1)
1567 CCGC
BbsI 54 GTCTTC GAAGAC(2/6)
710 GTCTTC
BpuAI 54 GTCTTC GAAGAC(2/6)
710 GTCTTC
BsrDI 207 CATTGC GCAATG(2/0)
692 GCAATG
Cac8I 912 GCTAGC GC[ACGT]^[ACGT]GC
1565 GCCCGC
CfoI 131 GCGC GCG^C
640 GCGC
DpnII 1234 GATC ^GATC
1383 GATC
DraII 1091 GGGACCC [GA]G^G[ACGT]CC[CT]
1518 GGGCCCT
EcoNI 393 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
414 CCTTTATCAGG
EcoO109I 1091 GGGACCC [GA]G^G[ACGT]CC[CT]
1518 GGGCCCT
EcoT14I 511 CCAAGG C^C[AT][AT]GG
917 CCATGG
FauI 268 CCCGC CCCGC(4/6)
1566 CCCGC
HhaI 131 GCGC GCG^C
640 GCGC
HinP1I 131 GCGC G^CGC
640 GCGC
HindIII 123 AAGCTT A^AGCTT
214 AAGCTT
MboI 1234 GATC ^GATC
1383 GATC
MspA1I 1347 CAGCTG C[AC]G^C[GT]G
1495 CAGCTG
NdeII 1234 GATC ^GATC
1383 GATC
NspI 834 ACATGT [GA]CATG^[CT]
1082 ACATGC
PleI 1252 GACTC GAGTC(4/5)
956 GAGTC
PvuII 1347 CAGCTG CAG^CTG
1495 CAGCTG
SacI 301 GAGCTC GAGCT^C
1615 GAGCTC
Sau3AI 1234 GATC ^GATC
1383 GATC
SmlI 126 CTTAAG C^T[CT][GA]AG
684 CTTGAG
SspI 852 AATATT AAT^ATT
1268 AATATT
StyI 511 CCAAGG C^C[AT][AT]GG
917 CCATGG
TfiI 782 GATTC G^A[AT]TC
1009 GAATC
Tsp45I 144 GTCAC ^GT[GC]AC
176 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AcsI AfaI AluI AlwNI ApoI
AspHI AvaII BanII BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeIII MboII MnlI MseI
MslI MvaI MwoI NlaIII NlaIV
PflMI RsaI Sau96I ScrFI SfaNI
Tru9I TseI Tsp509I TspRI Van91I