Restriction map for
pF1KSDA1200
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# NO CUT ENZYMES ( 77 enzymes) #
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AatII Acc65I AcsI AflII AgeI
AhdI AocI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BbrPI BcgI BclI BfrI
BlnI BsaBI BsiWI BsmI BspLU11I
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV FbaI FseI HpaI
KpnI MamI MfeI MseI MunI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI PvuI SalI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspI SwaI Tru9I Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflIII 3255 ACGCGT A^C[GA][CT]GT
ApaI 3548 GGGCCC GGGCC^C
AviII 2199 TGCGCA TGC^GCA
BbeI 21 GGCGCC GGCGC^C
BciVI 2352 GGATAC GTATCC(6/5)
BsaAI 2355 TACGTG [CT]AC^GT[GA]
BsaI 181 GAGACC GGTCTC(1/5)
BsiEI 1958 CGGCCG CG[GA][CT]^CG
Bsp1407I 3272 TGTACA T^GTACA
BspDI 2219 ATCGAT AT^CGAT
BspHI 2702 TCATGA T^CATGA
BsrGI 3272 TGTACA T^GTACA
ClaI 2219 ATCGAT AT^CGAT
EagI 1958 CGGCCG C^GGCCG
EclXI 1958 CGGCCG C^GGCCG
Eco52I 1958 CGGCCG C^GGCCG
EcoT22I 804 ATGCAT ATGCA^T
FspI 2199 TGCGCA TGC^GCA
HincII 3585 GTTGAC GT[CT]^[GA]AC
HindII 3585 GTTGAC GT[CT]^[GA]AC
KasI 21 GGCGCC G^GCGCC
KspI 961 CCGCGG CCGC^GG
MluI 3255 ACGCGT A^CGCGT
NaeI 24 GCCGGC GCC^GGC
NarI 21 GGCGCC GG^CGCC
NcoI 1014 CCATGG C^CATGG
NgoMIV 24 GCCGGC G^CCGGC
NsiI 804 ATGCAT ATGCA^T
PshAI 3252 GACACGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2702 TCATGA T^CATGA
SacII 961 CCGCGG CCGC^GG
SbfI 3165 CCTGCAGG CCTGCA^GG
SfoI 21 GGCGCC GGC^GCC
Sse8387I 3165 CCTGCAGG CCTGCA^GG
SspBI 3272 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccIII 1850 TCCGGA T^CCGGA
3914 TCCGGA
AclI 3822 AACGTT AA^CGTT
4083 AACGTT
Alw44I 3001 GTGCAC G^TGCAC
3790 GTGCAC
ApaLI 3001 GTGCAC G^TGCAC
3790 GTGCAC
Asp700I 502 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2133 GAAAATCTTC
BamHI 481 GGATCC G^GATCC
1988 GGATCC
BbvCI 2791 CCTCAGC CCTCAGC(-5/-2)
3564 GCTGAGG
BglI 3412 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3464 GCCCTGGAGGC
BseAI 1850 TCCGGA T^CCGGA
3914 TCCGGA
BsmBI 1587 CGTCTC CGTCTC(1/5)
3313 GAGACG
BspEI 1850 TCCGGA T^CCGGA
3914 TCCGGA
BsrDI 3605 CATTGC GCAATG(2/0)
3973 CATTGC
BsrFI 24 GCCGGC [GA]^CCGG[CT]
2598 GCCGGT
Cfr10I 24 GCCGGC [GA]^CCGG[CT]
2598 GCCGGT
CpoI 2905 CGGACCG CG^G[AT]CCG
3317 CGGACCG
HindIII 421 AAGCTT A^AGCTT
811 AAGCTT
MroI 1850 TCCGGA T^CCGGA
3914 TCCGGA
Psp1406I 3822 AACGTT AA^CGTT
4083 AACGTT
RsrII 2905 CGGACCG CG^G[AT]CCG
3317 CGGACCG
ScaI 1995 AGTACT AGT^ACT
2735 AGTACT
SmaI 1109 CCCGGG CCC^GGG
1568 CCCGGG
SphI 1295 GCATGC GCATG^C
1333 GCATGC
StuI 1052 AGGCCT AGG^CCT
2540 AGGCCT
TaqI 1899 TCGA T^CGA
2220 TCGA
TthHB8I 1899 TCGA T^CGA
2220 TCGA
XmaCI 1109 CCCGGG C^CCGGG
1568 CCCGGG
XmaI 1109 CCCGGG C^CCGGG
1568 CCCGGG
XmnI 502 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2133 GAAAATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII