Restriction map for
pF1KSDA1202
##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI BcgI BclI BglII
BsaBI BsiEI BsiWI BsmBI Bsp1407I
BspHI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FbaI HgaI HindIII HpaI KpnI
KspI MamI MfeI MluI MunI
MvnI NdeI NotI NruI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 4469 GTCTAC GT^[AC][GT]AC
AccIII 242 TCCGGA T^CCGGA
AflII 73 CTTAAG C^TTAAG
Alw44I 1270 GTGCAC G^TGCAC
ApaLI 1270 GTGCAC G^TGCAC
Asp700I 3983 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3158 TGCGCA TGC^GCA
AvrII 3915 CCTAGG C^CTAGG
BamHI 1102 GGATCC G^GATCC
BbrPI 4294 CACGTG CAC^GTG
BciVI 781 GGATAC GTATCC(6/5)
BfrI 73 CTTAAG C^TTAAG
BglI 1052 GCCAACAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3915 CCTAGG C^CTAGG
BlpI 3703 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3703 GCTCAGC GC^T[ACGT]AGC
BsaAI 4294 CACGTG [CT]AC^GT[GA]
BsaWI 242 TCCGGA [AT]^CCGG[AT]
BseAI 242 TCCGGA T^CCGGA
BspDI 4201 ATCGAT AT^CGAT
BspEI 242 TCCGGA T^CCGGA
BspLU11I 539 ACATGT A^CATGT
BsrDI 439 CATTGC GCAATG(2/0)
BstAPI 1263 GCAGCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 3703 GCTCAGC GC^T[ACGT]AGC
ClaI 4201 ATCGAT AT^CGAT
FseI 685 GGCCGGCC GGCCGG^CC
FspI 3158 TGCGCA TGC^GCA
MroI 242 TCCGGA T^CCGGA
NaeI 686 GCCGGC GCC^GGC
NgoMIV 686 GCCGGC G^CCGGC
NheI 1170 GCTAGC G^CTAGC
PmaCI 4294 CACGTG CAC^GTG
PmlI 4294 CACGTG CAC^GTG
SexAI 2098 ACCTGGT A^CC[AT]GGT
SspI 3847 AATATT AAT^ATT
XbaI 2595 TCTAGA T^CTAGA
XmnI 3983 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 2465 GGCGCC G[GA]^CG[CT]C
3195 GGCGCC
AflIII 407 ACGTGT A^C[GA][CT]GT
539 ACATGT
AspI 1335 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3258 GACCCAGTC
BbeI 2465 GGCGCC GGCGC^C
3195 GGCGCC
BbsI 2937 GAAGAC GAAGAC(2/6)
1989 GTCTTC
BpuAI 2937 GAAGAC GAAGAC(2/6)
1989 GTCTTC
BsaHI 2465 GGCGCC G[GA]^CG[CT]C
3195 GGCGCC
BsrBI 100 CCGCTC CCGCTC(-3/-3)
1728 CCGCTC
DraIII 2097 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3608 CACAAAGTG
DrdI 2940 GACCAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4132 GACAAAGTGGTC
EcoT22I 349 ATGCAT ATGCA^T
2790 ATGCAT
HaeII 2465 GGCGCC [GA]GCGC^[CT]
3195 GGCGCC
Hin1I 2465 GGCGCC G[GA]^CG[CT]C
3195 GGCGCC
HincII 3931 GTTGAC GT[CT]^[GA]AC
4141 GTCAAC
HindII 3931 GTTGAC GT[CT]^[GA]AC
4141 GTCAAC
KasI 2465 GGCGCC G^GCGCC
3195 GGCGCC
NarI 2465 GGCGCC GG^CGCC
3195 GGCGCC
NcoI 2222 CCATGG C^CATGG
2474 CCATGG
NsiI 349 ATGCAT ATGCA^T
2790 ATGCAT
PaeR7I 2836 CTCGAG C^TCGAG
3594 CTCGAG
PflFI 1335 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3258 GACCCAGTC
SapI 4441 GAAGAGC GCTCTTC(1/4)
1956 GCTCTTC
SfoI 2465 GGCGCC GGC^GCC
3195 GGCGCC
Tth111I 1335 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3258 GACCCAGTC
XhoI 2836 CTCGAG C^TCGAG
3594 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI