Restriction map for pF1KSDA1225
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       AccII       AccIII      AcyI        AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AspEI       AspI        AvaI        AviII       
AvrII       BalI        BbeI        BbrPI       BcgI        
BcnI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsaWI       BseAI       BsiWI       BsmBI       
BsmFI       BsoBI       BspDI       BspEI       BsrBI       
BsrFI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       KasI        KspI        MluI        
MluNI       MroI        MscI        MspI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PvuI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SwaI        
Tth111I     XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

Acc65I         33  GGTACC               G^GTACC
AccI           47  GTCTAC               GT^[AC][GT]AC
AclI          246  AACGTT               AA^CGTT
Asp718I        33  GGTACC               G^GTACC
BamHI        3861  GGATCC               G^GATCC
BciVI        2436  GTATCC               GTATCC(6/5)
BglI         3663  GCCTCTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2562  AGATCT               A^GATCT
BsiEI        3186  CGACCG               CG[GA][CT]^CG
BsmI         3540  GCATTC               GAATGC(1/-1)
BspHI        2166  TCATGA               T^CATGA
BspMI        2772  GCAGGT               ACCTGC(4/8)
BssSI        3578  CTCGTG               CACGAG(-5/-1)
BstAPI        256  GCATCCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII        2877  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII       2967  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        3808  CCTAGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     2877  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         583  GAATTC               G^AATTC
EcoRV        1445  GATATC               GAT^ATC
EcoT22I      3413  ATGCAT               ATGCA^T
HpaI         1765  GTTAAC               GTT^AAC
KpnI           33  GGTACC               GGTAC^C
MaeII         247  ACGT                 A^CGT
NsiI         3413  ATGCAT               ATGCA^T
Psp1406I      246  AACGTT               AA^CGTT
RcaI         2166  TCATGA               T^CATGA
SpeI         3499  ACTAGT               A^CTAGT
StuI         3670  AGGCCT               AGG^CCT
XbaI         3733  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflII         969  CTTAAG               C^TTAAG
             1471  CTTAAG              
AflIII        751  ACATGT               A^C[GA][CT]GT
             1274  ACATGT              
AlwNI        1721  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             3248  CAGCCTCTG           
AocI         3673  CCTCAGG              CC^T[ACGT]AGG
             3706  CCTCAGG             
BanI           33  GGTACC               G^G[CT][GA]CC
             1416  GGCACC              
BanII        1370  GGGCTC               G[GA]GC[CT]^C
             3287  GGGCTC              
BbvCI        2674  CCTCAGC              CCTCAGC(-5/-2)
             3019  CCTCAGC             
BclI          987  TGATCA               T^GATCA
             2760  TGATCA              
BfrI          969  CTTAAG               C^TTAAG
             1471  CTTAAG              
BsaBI         160  GATGCTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2839  GATTCAAATC          
BsgI         2900  CTGCAC               GTGCAG(16/14)
             3383  CTGCAC              
Bsp1407I     1503  TGTACA               T^GTACA
             1671  TGTACA              
BspLU11I      751  ACATGT               A^CATGT
             1274  ACATGT              
BsrGI        1503  TGTACA               T^GTACA
             1671  TGTACA              
Bsu36I       3673  CCTCAGG              CC^T[ACGT]AGG
             3706  CCTCAGG             
DraI         1437  TTTAAA               TTT^AAA
             2133  TTTAAA              
Eco81I       3673  CCTCAGG              CC^T[ACGT]AGG
             3706  CCTCAGG             
EcoT14I       140  CCTTGG               C^C[AT][AT]GG
             1291  CCAAGG              
FbaI          987  TGATCA               T^GATCA
             2760  TGATCA              
HindIII       935  AAGCTT               A^AGCTT
             2379  AAGCTT              
MamI          160  GATGCTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2839  GATTCAAATC          
NspI          751  ACATGT               [GA]CATG^[CT]
             1274  ACATGT              
SspBI        1503  TGTACA               T^GTACA
             1671  TGTACA              
StyI          140  CCTTGG               C^C[AT][AT]GG
             1291  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AseI        AsnI        Asp700I     AspHI       
AvaII       BbsI        BbvI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HincII      HindII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MfeI        MflI        MnlI        MseI        
MslI        MspA1I      MunI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PshBI       PstI        PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SmlI        SspI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       XmnI        
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