################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccII AccIII AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BcgI BcnI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaII KasI KspI MluI MluNI MroI MscI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 33 GGTACC G^GTACC AccI 47 GTCTAC GT^[AC][GT]AC AclI 246 AACGTT AA^CGTT Asp718I 33 GGTACC G^GTACC BamHI 3861 GGATCC G^GATCC BciVI 2436 GTATCC GTATCC(6/5) BglI 3663 GCCTCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2562 AGATCT A^GATCT BsiEI 3186 CGACCG CG[GA][CT]^CG BsmI 3540 GCATTC GAATGC(1/-1) BspHI 2166 TCATGA T^CATGA BspMI 2772 GCAGGT ACCTGC(4/8) BssSI 3578 CTCGTG CACGAG(-5/-1) BstAPI 256 GCATCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraII 2877 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 2967 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 3808 CCTAGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 2877 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 583 GAATTC G^AATTC EcoRV 1445 GATATC GAT^ATC EcoT22I 3413 ATGCAT ATGCA^T HpaI 1765 GTTAAC GTT^AAC KpnI 33 GGTACC GGTAC^C MaeII 247 ACGT A^CGT NsiI 3413 ATGCAT ATGCA^T Psp1406I 246 AACGTT AA^CGTT RcaI 2166 TCATGA T^CATGA SpeI 3499 ACTAGT A^CTAGT StuI 3670 AGGCCT AGG^CCT XbaI 3733 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflII 969 CTTAAG C^TTAAG 1471 CTTAAG AflIII 751 ACATGT A^C[GA][CT]GT 1274 ACATGT AlwNI 1721 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG 3248 CAGCCTCTG AocI 3673 CCTCAGG CC^T[ACGT]AGG 3706 CCTCAGG BanI 33 GGTACC G^G[CT][GA]CC 1416 GGCACC BanII 1370 GGGCTC G[GA]GC[CT]^C 3287 GGGCTC BbvCI 2674 CCTCAGC CCTCAGC(-5/-2) 3019 CCTCAGC BclI 987 TGATCA T^GATCA 2760 TGATCA BfrI 969 CTTAAG C^TTAAG 1471 CTTAAG BsaBI 160 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2839 GATTCAAATC BsgI 2900 CTGCAC GTGCAG(16/14) 3383 CTGCAC Bsp1407I 1503 TGTACA T^GTACA 1671 TGTACA BspLU11I 751 ACATGT A^CATGT 1274 ACATGT BsrGI 1503 TGTACA T^GTACA 1671 TGTACA Bsu36I 3673 CCTCAGG CC^T[ACGT]AGG 3706 CCTCAGG DraI 1437 TTTAAA TTT^AAA 2133 TTTAAA Eco81I 3673 CCTCAGG CC^T[ACGT]AGG 3706 CCTCAGG EcoT14I 140 CCTTGG C^C[AT][AT]GG 1291 CCAAGG FbaI 987 TGATCA T^GATCA 2760 TGATCA HindIII 935 AAGCTT A^AGCTT 2379 AAGCTT MamI 160 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2839 GATTCAAATC NspI 751 ACATGT [GA]CATG^[CT] 1274 ACATGT SspBI 1503 TGTACA T^GTACA 1671 TGTACA StyI 140 CCTTGG C^C[AT][AT]GG 1291 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII FauI Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MunI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI PshBI PstI PvuII RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI