Restriction map for
pF1KSDA1234
##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbsI BciVI
BclI BfrI BglII BlnI BpuAI
BsaBI BsaI BsaWI BseAI BsmBI
BsmI BspDI BspEI BspLU11I Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV FbaI
FseI FspI HincII HindII HpaI
KpnI MamI MfeI MflI MluI
MroI MunI NdeI NheI NotI
NruI NspI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I RsrII
SalI SbfI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TthHB8I
XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcsI 799 AAATTC [GA]^AATT[CT]
AlwI 15 GATCC GGATC(4/5)
AlwNI 326 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 927 CCTGAGG CC^T[ACGT]AGG
ApoI 799 AAATTC [GA]^AATT[CT]
Asp700I 240 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1147 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 490 CACGTG CAC^GTG
BcgI 1640 CGACGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1651 CTAG C^TAG
BglI 82 GCCGTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiWI 35 CGTACG C^GTACG
Bsp1407I 1811 TGTACA T^GTACA
BspHI 770 TCATGA T^CATGA
BspMI 1999 ACCTGC ACCTGC(4/8)
BsrBI 1053 GAGCGG CCGCTC(-3/-3)
BsrDI 870 CATTGC GCAATG(2/0)
BsrGI 1811 TGTACA T^GTACA
BssHII 920 GCGCGC G^CGCGC
BssSI 1569 CACGAG CACGAG(-5/-1)
BstXI 1661 CCATCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 927 CCTGAGG CC^T[ACGT]AGG
EagI 287 CGGCCG C^GGCCG
EarI 240 GAAGAG CTCTTC(1/4)
EclXI 287 CGGCCG C^GGCCG
Eco52I 287 CGGCCG C^GGCCG
Eco81I 927 CCTGAGG CC^T[ACGT]AGG
EcoNI 319 CCTCTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 413 ATGCAT ATGCA^T
Ksp632I 240 GAAGAG CTCTTC(1/4)
MaeI 1651 CTAG C^TAG
MseI 368 TTAA T^TAA
NsiI 413 ATGCAT ATGCA^T
PflFI 1147 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2073 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 490 CACGTG CAC^GTG
PmlI 490 CACGTG CAC^GTG
PshAI 310 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 425 CGATCG CGAT^CG
RcaI 770 TCATGA T^CATGA
SapI 240 GAAGAGC GCTCTTC(1/4)
ScaI 647 AGTACT AGT^ACT
SexAI 1487 ACCAGGT A^CC[AT]GGT
SspBI 1811 TGTACA T^GTACA
TfiI 1000 GAATC G^A[AT]TC
Tru9I 368 TTAA T^TAA
Tth111I 1147 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2073 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 240 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 292 GGCGCC G[GA]^CG[CT]C
825 GGCGCC
ApaI 614 GGGCCC GGGCC^C
1115 GGGCCC
BalI 152 TGGCCA TGG^CCA
2111 TGGCCA
BbeI 292 GGCGCC GGCGC^C
825 GGCGCC
BbvCI 1543 CCTCAGC CCTCAGC(-5/-2)
2011 GCTGAGG
BsaAI 33 CACGTA [CT]AC^GT[GA]
490 CACGTG
BsaHI 292 GGCGCC G[GA]^CG[CT]C
825 GGCGCC
BsrFI 289 GCCGGC [GA]^CCGG[CT]
515 GCCGGC
Cfr10I 289 GCCGGC [GA]^CCGG[CT]
515 GCCGGC
DpnII 15 GATC ^GATC
426 GATC
DraIII 1831 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1907 CACACTGTG
HaeII 292 GGCGCC [GA]GCGC^[CT]
825 GGCGCC
HgaI 1084 GACGC GACGC(5/10)
1641 GACGC
Hin1I 292 GGCGCC G[GA]^CG[CT]C
825 GGCGCC
HindIII 199 AAGCTT A^AGCTT
2132 AAGCTT
KasI 292 GGCGCC G^GCGCC
825 GGCGCC
MaeII 34 ACGT A^CGT
491 ACGT
MboI 15 GATC ^GATC
426 GATC
MluNI 152 TGGCCA TGG^CCA
2111 TGGCCA
MscI 152 TGGCCA TGG^CCA
2111 TGGCCA
NaeI 289 GCCGGC GCC^GGC
515 GCCGGC
NarI 292 GGCGCC GG^CGCC
825 GGCGCC
NdeII 15 GATC ^GATC
426 GATC
NgoMIV 289 GCCGGC G^CCGGC
515 GCCGGC
PpuMI 1248 AGGACCC [GA]G^G[AT]CC[CT]
1625 GGGACCT
PvuII 312 CAGCTG CAG^CTG
742 CAGCTG
SacI 270 GAGCTC GAGCT^C
1553 GAGCTC
Sau3AI 15 GATC ^GATC
426 GATC
SfoI 292 GGCGCC GGC^GCC
825 GGCGCC
SmaI 722 CCCGGG CCC^GGG
2086 CCCGGG
Tsp509I 800 AATT ^AATT
1024 AATT
XcmI 546 CCAGGTGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2099 CCAAAAGTGCCTTGG
XmaCI 722 CCCGGG C^CCGGG
2086 CCCGGG
XmaI 722 CCCGGG C^CCGGG
2086 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
ApaLI AspHI AvaI AvaII BanI
BanII BbvI BcnI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI KspI MaeIII MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NlaIII NlaIV
PleI PstI RsaI SacII Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I TspRI