Restriction map for
pF1KSDA1242
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BbrPI BbsI BbvCI BcgI BclI
BfrI BlnI BpuAI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MfeI MroI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp509I Tth111I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AhdI 580 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 472 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1398 CCTGAGG CC^T[ACGT]AGG
Aor51HI 404 AGCGCT AGC^GCT
AspEI 580 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1307 TGGCCA TGG^CCA
BamHI 1472 GGATCC G^GATCC
BbeI 20 GGCGCC GGCGC^C
BciVI 1142 GTATCC GTATCC(6/5)
BfaI 613 CTAG C^TAG
BglI 1069 GCCGTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 384 CACGTA [CT]AC^GT[GA]
BsaBI 36 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 979 CTCCTC GAGGAG(10/8)
BsmBI 1388 GAGACG CGTCTC(1/5)
BsmI 837 GAATGC GAATGC(1/-1)
BspMI 619 ACCTGC ACCTGC(4/8)
BsrDI 1348 GCAATG GCAATG(2/0)
BstAPI 1101 GCACGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1232 CCAACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1398 CCTGAGG CC^T[ACGT]AGG
DraII 323 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 1434 GACAAAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 701 CCGTGG C^C[GA][CT]GG
EagI 1067 CGGCCG C^GGCCG
Eam1105I 580 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1462 CTCTTC CTCTTC(1/4)
EclXI 1067 CGGCCG C^GGCCG
Eco47III 404 AGCGCT AGC^GCT
Eco52I 1067 CGGCCG C^GGCCG
Eco81I 1398 CCTGAGG CC^T[ACGT]AGG
EcoNI 1296 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 323 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 230 CCAAGG C^C[AT][AT]GG
KasI 20 GGCGCC G^GCGCC
Ksp632I 1462 CTCTTC CTCTTC(1/4)
MaeI 613 CTAG C^TAG
MamI 36 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1307 TGGCCA TGG^CCA
MscI 1307 TGGCCA TGG^CCA
MseI 191 TTAA T^TAA
NaeI 304 GCCGGC GCC^GGC
NarI 20 GGCGCC GG^CGCC
NgoMIV 304 GCCGGC G^CCGGC
PaeR7I 1026 CTCGAG C^TCGAG
PflMI 1079 CCATCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 323 AGGACCC [GA]G^G[AT]CC[CT]
SexAI 725 ACCTGGT A^CC[AT]GGT
SfiI 1068 GGCCGTGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 20 GGCGCC GGC^GCC
StyI 230 CCAAGG C^C[AT][AT]GG
TfiI 1020 GAATC G^A[AT]TC
Tru9I 191 TTAA T^TAA
Van91I 1079 CCATCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1026 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 66 ACGCGT A^C[GA][CT]GT
816 ACGCGT
BglII 509 AGATCT A^GATCT
761 AGATCT
BlpI 600 GCTCAGC GC^T[ACGT]AGC
841 GCTGAGC
Bpu1102I 600 GCTCAGC GC^T[ACGT]AGC
841 GCTGAGC
BsaI 134 GAGACC GGTCTC(1/5)
1393 GAGACC
BsaWI 221 ACCGGA [AT]^CCGG[AT]
482 TCCGGT
BsiEI 867 CGACCG CG[GA][CT]^CG
1067 CGGCCG
BsrFI 304 GCCGGC [GA]^CCGG[CT]
935 ACCGGC
CelII 600 GCTCAGC GC^T[ACGT]AGC
841 GCTGAGC
Cfr10I 304 GCCGGC [GA]^CCGG[CT]
935 ACCGGC
EaeI 1067 CGGCCG [CT]^GGCC[GA]
1307 TGGCCA
MaeII 385 ACGT A^CGT
1412 ACGT
MluI 66 ACGCGT A^CGCGT
816 ACGCGT
NlaIII 795 CATG CATG^
873 CATG
PleI 688 GACTC GAGTC(4/5)
1441 GAGTC
PvuII 847 CAGCTG CAG^CTG
1130 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TseI Tsp45I TspRI TthHB8I XhoII