Restriction map for
pF1KSDA1258
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BmyI BpmI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cfr10I CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MflI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AlwI 90 GGATC GGATC(4/5)
AseI 1063 ATTAAT AT^TAAT
AsnI 1063 ATTAAT AT^TAAT
Asp700I 864 GAACGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 65 GGACC G^G[AT]CC
BalI 161 TGGCCA TGG^CCA
BbsI 1102 GTCTTC GAAGAC(2/6)
BclI 1196 TGATCA T^GATCA
BlpI 921 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 921 GCTCAGC GC^T[ACGT]AGC
BpuAI 1102 GTCTTC GAAGAC(2/6)
BsaI 544 GAGACC GGTCTC(1/5)
BspDI 558 ATCGAT AT^CGAT
BsrBI 8 CCGCTC CCGCTC(-3/-3)
BsrDI 684 CATTGC GCAATG(2/0)
BstAPI 500 GCAAAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 486 GCGGGC GC[ACGT]^[ACGT]GC
CelII 921 GCTCAGC GC^T[ACGT]AGC
CfoI 28 GCGC GCG^C
Cfr13I 65 GGACC G^G[ACGT]CC
ClaI 558 ATCGAT AT^CGAT
DsaI 74 CCATGG C^C[GA][CT]GG
EaeI 161 TGGCCA [CT]^GGCC[GA]
EcoT14I 74 CCATGG C^C[AT][AT]GG
FbaI 1196 TGATCA T^GATCA
HaeIII 162 GGCC GG^CC
HhaI 28 GCGC GCG^C
HinP1I 28 GCGC G^CGC
MluNI 161 TGGCCA TGG^CCA
MscI 161 TGGCCA TGG^CCA
NcoI 74 CCATGG C^CATGG
NlaIV 1341 GGTTCC GG[ACGT]^[ACGT]CC
PacI 1060 TTAATTAA TTAAT^TAA
PshAI 45 GACGTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1063 ATTAAT AT^TAAT
SapI 1031 GAAGAGC GCTCTTC(1/4)
Sau96I 65 GGACC G^G[ACGT]CC
SspI 1306 AATATT AAT^ATT
StyI 74 CCATGG C^C[AT][AT]GG
Tsp45I 628 GTGAC ^GT[GC]AC
XbaI 610 TCTAGA T^CTAGA
XcmI 292 CCACTCTTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 864 GAACGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 475 AAATTT [GA]^AATT[CT]
1180 GAATTT
AfaI 315 GTAC GT^AC
983 GTAC
ApoI 475 AAATTT [GA]^AATT[CT]
1180 GAATTT
BbvI 84 GCTGC GCAGC(8/12)
839 GCTGC
BseRI 97 CTCCTC GAGGAG(10/8)
881 GAGGAG
BsmAI 544 GAGAC GTCTC(1/5)
658 GAGAC
EarI 1031 GAAGAG CTCTTC(1/4)
1312 GAAGAG
FauI 87 GCGGG CCCGC(4/6)
486 GCGGG
Ksp632I 1031 GAAGAG CTCTTC(1/4)
1312 GAAGAG
MaeIII 419 GTTAC ^GT[ACGT]AC
628 GTGAC
MseI 1060 TTAA T^TAA
1064 TTAA
MspA1I 114 CAGCGG C[AC]G^C[GT]G
484 CAGCGG
PflMI 164 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1131 CCAAGCCCTGG
PleI 442 GACTC GAGTC(4/5)
1216 GACTC
PstI 326 CTGCAG CTGCA^G
851 CTGCAG
RsaI 315 GTAC GT^AC
983 GTAC
SfcI 326 CTGCAG C^T[GA][CT]AG
851 CTGCAG
TfiI 338 GATTC G^A[AT]TC
556 GAATC
Tru9I 1060 TTAA T^TAA
1064 TTAA
TseI 84 GCTGC G^C[AT]GC
839 GCTGC
Van91I 164 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1131 CCAAGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BfaI BsaJI BsiYI
BslI BsmFI BspMI BsrI BssKI
BstF5I BstNI DdeI DpnII EcoRII
Fnu4HI FokI HinfI HphI ItaI
MaeI MaeII MboI MboII MnlI
MslI MvaI MwoI NdeII NlaIII
Sau3AI ScrFI SfaNI TaqI Tsp509I
TspRI TthHB8I