Restriction map for
pF1KSDA1263
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AspEI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI TthHB8I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1681 GGTACC G^GTACC
AflIII 1352 ACATGT A^C[GA][CT]GT
Alw44I 337 GTGCAC G^TGCAC
ApaLI 337 GTGCAC G^TGCAC
Asp700I 1350 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1681 GGTACC G^GTACC
BanII 2305 GAGCTC G[GA]GC[CT]^C
BciVI 1032 GTATCC GTATCC(6/5)
BclI 1656 TGATCA T^GATCA
BcnI 1026 CCGGG CC^[GC]GG
BlpI 1671 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1671 GCTAAGC GC^T[ACGT]AGC
BsaI 825 GAGACC GGTCTC(1/5)
BseRI 2168 GAGGAG GAGGAG(10/8)
BsmI 718 GCATTC GAATGC(1/-1)
BspLU11I 1352 ACATGT A^CATGT
BspMI 955 ACCTGC ACCTGC(4/8)
BsrDI 1199 GCAATG GCAATG(2/0)
BstAPI 250 GCAGAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 927 GGTTACC G^GT[ACGT]ACC
BstPI 927 GGTTACC G^GT[ACGT]ACC
BstXI 2397 CCAGGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1671 GCTAAGC GC^T[ACGT]AGC
CfoI 1206 GCGC GCG^C
DraII 907 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 907 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 927 GGTTACC G^GT[ACGT]ACC
EcoRI 313 GAATTC G^AATTC
FbaI 1656 TGATCA T^GATCA
HhaI 1206 GCGC GCG^C
HinP1I 1206 GCGC G^CGC
HindIII 1147 AAGCTT A^AGCTT
KpnI 1681 GGTACC GGTAC^C
MspA1I 1511 CAGCTG C[AC]G^C[GT]G
NciI 1026 CCGGG CC^[GC]GG
NspI 1352 ACATGT [GA]CATG^[CT]
PpuMI 907 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 770 CTGCAG CTGCA^G
PvuII 1511 CAGCTG CAG^CTG
SacI 2305 GAGCTC GAGCT^C
ScaI 200 AGTACT AGT^ACT
XcmI 1830 CCATATGAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1350 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 773 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1616 CAGACCCTG
AseI 586 ATTAAT AT^TAAT
2122 ATTAAT
AsnI 586 ATTAAT AT^TAAT
2122 ATTAAT
AvaII 267 GGACC G^G[AT]CC
908 GGTCC
BalI 1724 TGGCCA TGG^CCA
2103 TGGCCA
BanI 1079 GGTGCC G^G[CT][GA]CC
1681 GGTACC
BbvCI 1105 CCTCAGC CCTCAGC(-5/-2)
1474 GCTGAGG
BsaWI 2005 ACCGGA [AT]^CCGG[AT]
2378 ACCGGA
Cac8I 246 GCAAGC GC[ACGT]^[ACGT]GC
349 GCTTGC
DraI 400 TTTAAA TTT^AAA
547 TTTAAA
EaeI 1724 TGGCCA [CT]^GGCC[GA]
2103 TGGCCA
MaeII 279 ACGT A^CGT
941 ACGT
MluNI 1724 TGGCCA TGG^CCA
2103 TGGCCA
MscI 1724 TGGCCA TGG^CCA
2103 TGGCCA
NdeI 1831 CATATG CA^TATG
2087 CATATG
PflMI 833 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1761 CCAACCAGTGG
PshBI 586 ATTAAT AT^TAAT
2122 ATTAAT
SfcI 770 CTGCAG C^T[GA][CT]AG
1708 CTACAG
SmlI 2341 CTCAAG C^T[CT][GA]AG
2353 CTCAAG
SspI 551 AATATT AAT^ATT
853 AATATT
Tsp45I 281 GTCAC ^GT[GC]AC
2074 GTGAC
Van91I 833 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1761 CCAACCAGTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AspI BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
PflFI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TfiI Tru9I
TseI Tsp509I TspRI Tth111I XhoII