Restriction map for
pF1KSDA1264
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII AccI AclI AcsI AcyI
AflII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI AspI
AviII AvrII BbeI BbrPI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsgI BsiWI BspDI
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
CfoI ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KspI MfeI MluI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 1127 GGTACC G^GTACC
AccII 357 CGCG CG^CG
AccIII 1445 TCCGGA T^CCGGA
AflIII 1523 ACATGT A^C[GA][CT]GT
AhdI 1873 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1034 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1056 GGGCCC GGGCC^C
Asp700I 1288 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1127 GGTACC G^GTACC
AspEI 1873 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1346 TGGCCA TGG^CCA
BcgI 405 CGATACTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1487 TGATCA T^GATCA
BsaAI 511 CACGTA [CT]AC^GT[GA]
BsaI 1328 GAGACC GGTCTC(1/5)
BsaWI 1445 TCCGGA [AT]^CCGG[AT]
BseAI 1445 TCCGGA T^CCGGA
BsmBI 1458 GAGACG CGTCTC(1/5)
BsmI 980 GCATTC GAATGC(1/-1)
Bsp1407I 1526 TGTACA T^GTACA
BspEI 1445 TCCGGA T^CCGGA
BspHI 2135 TCATGA T^CATGA
BspLU11I 1523 ACATGT A^CATGT
BspMI 2107 GCAGGT ACCTGC(4/8)
BsrGI 1526 TGTACA T^GTACA
BstAPI 1382 GCATCGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 357 CGCG CG^CG
BstXI 182 CCATCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 910 TTTAAA TTT^AAA
DraIII 972 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1873 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1487 TGATCA T^GATCA
HincII 1519 GTTGAC GT[CT]^[GA]AC
HindII 1519 GTTGAC GT[CT]^[GA]AC
KpnI 1127 GGTACC GGTAC^C
MaeII 512 ACGT A^CGT
MluNI 1346 TGGCCA TGG^CCA
MroI 1445 TCCGGA T^CCGGA
MscI 1346 TGGCCA TGG^CCA
MvnI 357 CGCG CG^CG
NspI 1523 ACATGT [GA]CATG^[CT]
PaeR7I 666 CTCGAG C^TCGAG
PvuI 999 CGATCG CGAT^CG
RcaI 2135 TCATGA T^CATGA
SspBI 1526 TGTACA T^GTACA
StuI 536 AGGCCT AGG^CCT
Tsp509I 698 AATT ^AATT
XhoI 666 CTCGAG C^TCGAG
XmnI 1288 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 297 CCTAAGG CC^T[ACGT]AGG
1834 CCTGAGG
BamHI 468 GGATCC G^GATCC
1961 GGATCC
BbsI 201 GAAGAC GAAGAC(2/6)
807 GTCTTC
BcnI 1965 CCGGG CC^[GC]GG
2019 CCGGG
BpuAI 201 GAAGAC GAAGAC(2/6)
807 GTCTTC
BsaBI 463 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
994 GATTACGATC
BsiEI 425 CGACCG CG[GA][CT]^CG
999 CGATCG
BsrFI 97 ACCGGC [GA]^CCGG[CT]
1378 ACCGGC
BstYI 468 GGATCC [GA]^GATC[CT]
1961 GGATCC
Bsu36I 297 CCTAAGG CC^T[ACGT]AGG
1834 CCTGAGG
Cfr10I 97 ACCGGC [GA]^CCGG[CT]
1378 ACCGGC
DsaI 342 CCACGG C^C[GA][CT]GG
1187 CCACGG
EarI 444 GAAGAG CTCTTC(1/4)
1794 GAAGAG
Eco81I 297 CCTAAGG CC^T[ACGT]AGG
1834 CCTGAGG
EcoT22I 171 ATGCAT ATGCA^T
1389 ATGCAT
Ksp632I 444 GAAGAG CTCTTC(1/4)
1794 GAAGAG
MamI 463 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
994 GATTACGATC
MflI 468 GGATCC [GA]^GATC[CT]
1961 GGATCC
MseI 911 TTAA T^TAA
2098 TTAA
NciI 1965 CCGGG CC^[GC]GG
2019 CCGGG
NsiI 171 ATGCAT ATGCA^T
1389 ATGCAT
PpuMI 771 GGGACCC [GA]G^G[AT]CC[CT]
1991 AGGACCT
TaqI 667 TCGA T^CGA
1002 TCGA
Tru9I 911 TTAA T^TAA
2098 TTAA
TthHB8I 667 TCGA T^CGA
1002 TCGA
XbaI 709 TCTAGA T^CTAGA
1575 TCTAGA
XhoII 468 GGATCC [GA]^GATC[CT]
1961 GGATCC
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI TseI Tsp45I TspRI
XcmI