Restriction map for
pF1KSDA1266
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BanI BbeI
BbvCI BcgI BclI BcnI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NciI NcoI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 1258 AAATTT [GA]^AATT[CT]
AfaI 598 GTAC GT^AC
ApaI 1008 GGGCCC GGGCC^C
ApoI 1258 AAATTT [GA]^AATT[CT]
AseI 1327 ATTAAT AT^TAAT
AsnI 1327 ATTAAT AT^TAAT
AvaI 518 CCCGAG C^[CT]CG[GA]G
BamHI 378 GGATCC G^GATCC
BbsI 1215 GAAGAC GAAGAC(2/6)
BglI 1339 GCCATTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1215 GAAGAC GAAGAC(2/6)
BsgI 156 GTGCAG GTGCAG(16/14)
BsiEI 1312 CGGCCG CG[GA][CT]^CG
BsoBI 518 CCCGAG C^[CT]CG[GA]G
BspLU11I 764 ACATGT A^CATGT
BssSI 1136 CTCGTG CACGAG(-5/-1)
BstXI 452 CCAGAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 1312 CGGCCG [CT]^GGCC[GA]
EagI 1312 CGGCCG C^GGCCG
EclXI 1312 CGGCCG C^GGCCG
Eco52I 1312 CGGCCG C^GGCCG
EcoNI 946 CCTCTCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 296 GATATC GAT^ATC
EcoT14I 727 CCTTGG C^C[AT][AT]GG
HapII 1290 CCGG C^CGG
HindIII 1226 AAGCTT A^AGCTT
HpaII 1290 CCGG C^CGG
MspI 1290 CCGG C^CGG
NdeI 496 CATATG CA^TATG
NheI 658 GCTAGC G^CTAGC
PleI 1197 GAGTC GAGTC(4/5)
PshBI 1327 ATTAAT AT^TAAT
RsaI 598 GTAC GT^AC
SpeI 883 ACTAGT A^CTAGT
SphI 15 GCATGC GCATG^C
SspI 755 AATATT AAT^ATT
StyI 727 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 764 ACATGT A^C[GA][CT]GT
1151 ACGTGT
AlwNI 647 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
815 CAGAAGCTG
BanII 455 GAGCCC G[GA]GC[CT]^C
1008 GGGCCC
BciVI 213 GGATAC GTATCC(6/5)
546 GGATAC
BmyI 455 GAGCCC G[AGT]GC[ACT]^C
1008 GGGCCC
BsaJI 458 CCCTGG C^C[ACGT][ACGT]GG
727 CCTTGG
BsmI 1175 GAATGC GAATGC(1/-1)
1544 GCATTC
Bsp1286I 455 GAGCCC G[AGT]GC[ACT]^C
1008 GGGCCC
BstAPI 1170 GCAGGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1348 GCAGAATATGC
BstYI 378 GGATCC [GA]^GATC[CT]
1442 AGATCC
EarI 670 GAAGAG CTCTTC(1/4)
1099 GAAGAG
Ksp632I 670 GAAGAG CTCTTC(1/4)
1099 GAAGAG
MaeIII 111 GTCAC ^GT[ACGT]AC
1148 GTCAC
MflI 378 GGATCC [GA]^GATC[CT]
1442 AGATCC
MseI 368 TTAA T^TAA
1328 TTAA
PstI 509 CTGCAG CTGCA^G
1208 CTGCAG
SfaNI 1512 GATGC GCATC(5/9)
746 GCATC
TaqI 402 TCGA T^CGA
1473 TCGA
Tru9I 368 TTAA T^TAA
1328 TTAA
Tsp45I 111 GTCAC ^GT[GC]AC
1148 GTCAC
TthHB8I 402 TCGA T^CGA
1473 TCGA
XhoII 378 GGATCC [GA]^GATC[CT]
1442 AGATCC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI AvaII BbrPI
BbvI BfaI BsaAI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MboI MboII MnlI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PmaCI PmlI PpuMI
Sau3AI Sau96I ScrFI SfcI TfiI
TseI Tsp509I TspRI