Restriction map for
pF1KSDA1269
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BcgI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsiWI BsmBI BsmI Bsp1407I BspDI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MaeI
MseI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacI SalI SapI SbfI ScaI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tru9I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 54 TCCGGA T^CCGGA
AcsI 385 GAATTT [GA]^AATT[CT]
AflIII 1349 ACGCGT A^C[GA][CT]GT
AlwI 1338 GATCC GGATC(4/5)
AocI 1531 CCTGAGG CC^T[ACGT]AGG
ApaI 1449 GGGCCC GGGCC^C
ApoI 385 GAATTT [GA]^AATT[CT]
BalI 893 TGGCCA TGG^CCA
BanI 1248 GGCGCC G^G[CT][GA]CC
BbeI 1248 GGCGCC GGCGC^C
BbrPI 1085 CACGTG CAC^GTG
BbvCI 1017 CCTCAGC CCTCAGC(-5/-2)
BciVI 1288 GGATAC GTATCC(6/5)
BsaAI 1085 CACGTG [CT]AC^GT[GA]
BsaBI 970 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 54 TCCGGA [AT]^CCGG[AT]
BseAI 54 TCCGGA T^CCGGA
BspEI 54 TCCGGA T^CCGGA
BspHI 1031 TCATGA T^CATGA
BssSI 1579 CACGAG CACGAG(-5/-1)
BstAPI 1513 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1337 AGATCC [GA]^GATC[CT]
Bsu36I 1531 CCTGAGG CC^T[ACGT]AGG
Eco81I 1531 CCTGAGG CC^T[ACGT]AGG
EcoNI 1227 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 1248 GGCGCC G^GCGCC
MamI 970 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1496 CAATTG C^AATTG
MflI 1337 AGATCC [GA]^GATC[CT]
MluI 1349 ACGCGT A^CGCGT
MluNI 893 TGGCCA TGG^CCA
MroI 54 TCCGGA T^CCGGA
MscI 893 TGGCCA TGG^CCA
MunI 1496 CAATTG C^AATTG
NaeI 727 GCCGGC GCC^GGC
NarI 1248 GGCGCC GG^CGCC
NgoMIV 727 GCCGGC G^CCGGC
PflMI 762 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1085 CACGTG CAC^GTG
PmlI 1085 CACGTG CAC^GTG
PshAI 1041 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1031 TCATGA T^CATGA
SexAI 875 ACCTGGT A^CC[AT]GGT
SfiI 1104 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1248 GGCGCC GGC^GCC
SmlI 211 CTCAAG C^T[CT][GA]AG
SphI 866 GCATGC GCATG^C
TaqI 825 TCGA T^CGA
TthHB8I 825 TCGA T^CGA
Van91I 762 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1337 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Alw44I 22 GTGCAC G^TGCAC
391 GTGCAC
AlwNI 763 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1514 CAGGGGCTG
ApaLI 22 GTGCAC G^TGCAC
391 GTGCAC
AspI 502 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
739 GACGAGGTC
BanII 551 GAGCCC G[GA]GC[CT]^C
1449 GGGCCC
BbsI 93 GAAGAC GAAGAC(2/6)
658 GAAGAC
BglI 1105 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1138 GCCCCAGTGGC
BpuAI 93 GAAGAC GAAGAC(2/6)
658 GAAGAC
BsgI 853 GTGCAG GTGCAG(16/14)
1384 GTGCAG
BspMI 520 ACCTGC ACCTGC(4/8)
1119 GCAGGT
BsrBI 918 CCGCTC CCGCTC(-3/-3)
1582 GAGCGG
BstF5I 435 CATCC GGATG(2/0)
747 CATCC
CfoI 1147 GCGC GCG^C
1249 GCGC
DpnII 465 GATC ^GATC
1338 GATC
FokI 435 CATCC GGATG(9/13)
747 CATCC
HaeII 1146 GGCGCT [GA]GCGC^[CT]
1248 GGCGCC
HhaI 1147 GCGC GCG^C
1249 GCGC
HinP1I 1147 GCGC G^CGC
1249 GCGC
HinfI 6 GACTC G^A[ACGT]TC
472 GACTC
KspI 185 CCGCGG CCGC^GG
677 CCGCGG
MboI 465 GATC ^GATC
1338 GATC
NcoI 1109 CCATGG C^CATGG
1565 CCATGG
NdeII 465 GATC ^GATC
1338 GATC
NspI 366 GCATGT [GA]CATG^[CT]
866 GCATGC
PflFI 502 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
739 GACGAGGTC
PleI 6 GACTC GAGTC(4/5)
472 GACTC
PpuMI 806 AGGACCC [GA]G^G[AT]CC[CT]
941 GGGACCT
SacII 185 CCGCGG CCGC^GG
677 CCGCGG
Sau3AI 465 GATC ^GATC
1338 GATC
StuI 786 AGGCCT AGG^CCT
1475 AGGCCT
Tsp509I 386 AATT ^AATT
1497 AATT
Tth111I 502 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
739 GACGAGGTC
XcmI 1473 CCAGGCCTCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1547 CCACCATTGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BbvI BcnI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstNI BstUI BstXI
Cac8I Cfr10I Cfr13I DdeI DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HgaI Hin1I
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PstI PvuII RsaI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TseI Tsp45I TspRI XmaCI
XmaI