Restriction map for
pF1KSDA1322
##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BalI BamHI
BciVI BclI BfrI BglI BlnI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI HgaI
HindIII HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NdeI NgoMIV NheI
NotI NsiI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 62 GTCGAC GT^[AC][GT]AC
AcsI 1689 AAATTT [GA]^AATT[CT]
AcyI 108 GGCGCC G[GA]^CG[CT]C
ApaI 139 GGGCCC GGGCC^C
ApoI 1689 AAATTT [GA]^AATT[CT]
Asp700I 1869 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 108 GGCGCC G^G[CT][GA]CC
BbeI 108 GGCGCC GGCGC^C
BbrPI 253 CACGTG CAC^GTG
BbvCI 667 GCTGAGG CCTCAGC(-5/-2)
BcgI 1979 CGATCCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1139 AGATCT A^GATCT
BlpI 1235 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1235 GCTTAGC GC^T[ACGT]AGC
BsaAI 253 CACGTG [CT]AC^GT[GA]
BsaHI 108 GGCGCC G[GA]^CG[CT]C
BsmBI 120 CGTCTC CGTCTC(1/5)
BsmI 810 GAATGC GAATGC(1/-1)
BspLU11I 1463 ACATGT A^CATGT
BssHII 314 GCGCGC G^CGCGC
BstXI 1440 CCAGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1235 GCTTAGC GC^T[ACGT]AGC
DraII 1940 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 1501 CGGCCA [CT]^GGCC[GA]
EarI 1834 GAAGAG CTCTTC(1/4)
EcoO109I 1940 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1738 GATATC GAT^ATC
Hin1I 108 GGCGCC G[GA]^CG[CT]C
KasI 108 GGCGCC G^GCGCC
Ksp632I 1834 GAAGAG CTCTTC(1/4)
MslI 1905 CATTCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 108 GGCGCC GG^CGCC
NcoI 1638 CCATGG C^CATGG
NruI 1824 TCGCGA TCG^CGA
PaeR7I 946 CTCGAG C^TCGAG
PflMI 183 CCAGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 253 CACGTG CAC^GTG
PmlI 253 CACGTG CAC^GTG
PpuMI 1940 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1279 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 62 GTCGAC G^TCGAC
SfoI 108 GGCGCC GGC^GCC
SspI 690 AATATT AAT^ATT
Van91I 183 CCAGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 563 CCATTGTGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 946 CTCGAG C^TCGAG
XmnI 1869 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 1980 GATCC GGATC(4/5)
1757 GGATC
AviII 371 TGCGCA TGC^GCA
1707 TGCGCA
BsmAI 121 GTCTC GTCTC(1/5)
460 GTCTC
BspHI 660 TCATGA T^CATGA
1458 TCATGA
BspMI 86 ACCTGC ACCTGC(4/8)
1943 ACCTGC
BsrBI 292 GAGCGG CCGCTC(-3/-3)
355 GAGCGG
BsrDI 2027 CATTGC GCAATG(2/0)
518 GCAATG
BsrFI 170 ACCGGC [GA]^CCGG[CT]
1499 ACCGGC
BssSI 546 CACGAG CACGAG(-5/-1)
1267 CACGAG
BstYI 1139 AGATCT [GA]^GATC[CT]
1757 GGATCT
Cfr10I 170 ACCGGC [GA]^CCGG[CT]
1499 ACCGGC
DrdI 489 GACAGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
535 GACCTTGTGGTC
EcoT14I 1298 CCAAGG C^C[AT][AT]GG
1638 CCATGG
FspI 371 TGCGCA TGC^GCA
1707 TGCGCA
HincII 62 GTCGAC GT[CT]^[GA]AC
1650 GTTGAC
HindII 62 GTCGAC GT[CT]^[GA]AC
1650 GTTGAC
MflI 1139 AGATCT [GA]^GATC[CT]
1757 GGATCT
MseI 1255 TTAA T^TAA
1397 TTAA
MspA1I 980 CAGCTG C[AC]G^C[GT]G
1075 CAGCTG
NspI 1463 ACATGT [GA]CATG^[CT]
1526 GCATGT
PvuII 980 CAGCTG CAG^CTG
1075 CAGCTG
RcaI 660 TCATGA T^CATGA
1458 TCATGA
SacI 615 GAGCTC GAGCT^C
1270 GAGCTC
StyI 1298 CCAAGG C^C[AT][AT]GG
1638 CCATGG
Tru9I 1255 TTAA T^TAA
1397 TTAA
TspRI 558 CAGTG CA[GC]TG[ACGT][ACGT]^
1544 CAGTG
XbaI 1171 TCTAGA T^CTAGA
1411 TCTAGA
XhoII 1139 AGATCT [GA]^GATC[CT]
1757 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwNI AspHI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI TseI
Tsp45I Tsp509I TthHB8I