Restriction map for
pF1KSDA1334
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
AvaI AviII AvrII BalI BamHI
BbeI BcgI BclI BcnI BglI
BlnI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I ClaI CpoI DraII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRV
FauI FbaI FseI FspI HgaI
Hin1I HpaI KasI KspI MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PaeR7I PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 2349 GGTACC G^GTACC
AflII 2260 CTTAAG C^TTAAG
AocI 204 CCTCAGG CC^T[ACGT]AGG
ApaI 1182 GGGCCC GGGCC^C
Asp700I 2506 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2349 GGTACC G^GTACC
AspI 769 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 2448 GGTCC G^G[AT]CC
BanI 2349 GGTACC G^G[CT][GA]CC
BbrPI 193 CACGTG CAC^GTG
BbsI 2358 GTCTTC GAAGAC(2/6)
BciVI 652 GGATAC GTATCC(6/5)
BfaI 2002 CTAG C^TAG
BfrI 2260 CTTAAG C^TTAAG
BlpI 1969 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1969 GCTGAGC GC^T[ACGT]AGC
BpuAI 2358 GTCTTC GAAGAC(2/6)
BsaAI 193 CACGTG [CT]AC^GT[GA]
BsaI 2481 GGTCTC GGTCTC(1/5)
BsaWI 253 ACCGGA [AT]^CCGG[AT]
BseRI 1477 GAGGAG GAGGAG(10/8)
BsrDI 2344 GCAATG GCAATG(2/0)
BsrFI 62 ACCGGC [GA]^CCGG[CT]
BssSI 1567 CACGAG CACGAG(-5/-1)
Bsu36I 204 CCTCAGG CC^T[ACGT]AGG
CelII 1969 GCTGAGC GC^T[ACGT]AGC
Cfr10I 62 ACCGGC [GA]^CCGG[CT]
DraI 738 TTTAAA TTT^AAA
DraIII 221 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 77 CCGTGG C^C[GA][CT]GG
Eco81I 204 CCTCAGG CC^T[ACGT]AGG
EcoT14I 1124 CCAAGG C^C[AT][AT]GG
HindIII 1146 AAGCTT A^AGCTT
KpnI 2349 GGTACC GGTAC^C
MaeI 2002 CTAG C^TAG
MfeI 2056 CAATTG C^AATTG
MslI 145 CACGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2056 CAATTG C^AATTG
PacI 610 TTAATTAA TTAAT^TAA
PflFI 769 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 38 CCAATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 193 CACGTG CAC^GTG
PmlI 193 CACGTG CAC^GTG
SexAI 1200 ACCTGGT A^CC[AT]GGT
SmlI 2260 CTTAAG C^T[CT][GA]AG
SspI 2509 AATATT AAT^ATT
StuI 2687 AGGCCT AGG^CCT
StyI 1124 CCAAGG C^C[AT][AT]GG
Tth111I 769 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 38 CCAATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2001 TCTAGA T^CTAGA
XmnI 2506 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflIII 192 ACACGT A^C[GA][CT]GT
2066 ACGTGT
AlwI 516 GGATC GGATC(4/5)
1611 GGATC
AspHI 1338 GAGCTC G[AT]GC[AT]^C
2613 GAGCTC
BsiHKAI 1338 GAGCTC G[AT]GC[AT]^C
2613 GAGCTC
Bsp1407I 477 TGTACA T^GTACA
1952 TGTACA
BspHI 212 TCATGA T^CATGA
1823 TCATGA
BsrGI 477 TGTACA T^GTACA
1952 TGTACA
CfoI 263 GCGC GCG^C
2758 GCGC
DrdI 1276 GACAATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2061 GACAAACGTGTC
EcoRI 695 GAATTC G^AATTC
1289 GAATTC
HaeII 262 AGCGCC [GA]GCGC^[CT]
2757 GGCGCT
HapII 63 CCGG C^CGG
254 CCGG
HhaI 263 GCGC GCG^C
2758 GCGC
HinP1I 263 GCGC G^CGC
2758 GCGC
HincII 343 GTTGAC GT[CT]^[GA]AC
2136 GTTGAC
HindII 343 GTTGAC GT[CT]^[GA]AC
2136 GTTGAC
HpaII 63 CCGG C^CGG
254 CCGG
MaeII 194 ACGT A^CGT
2066 ACGT
MspI 63 CCGG C^CGG
254 CCGG
RcaI 212 TCATGA T^CATGA
1823 TCATGA
SacI 1338 GAGCTC GAGCT^C
2613 GAGCTC
SapI 3 GAAGAGC GCTCTTC(1/4)
2615 GCTCTTC
ScaI 985 AGTACT AGT^ACT
2892 AGTACT
SspBI 477 TGTACA T^GTACA
1952 TGTACA
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI BanII BbvCI BbvI BglII
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmAI BsmI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT22I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NsiI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII