Restriction map for
pF1KSDA1345
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI Alw44I AlwNI ApaI ApaLI
AscI AseI AsnI Asp718I AviII
AvrII BbeI BbvCI BlnI BsaHI
BsiEI BsiWI BsmBI BsmI BspDI
BspHI BspLU11I BssHII BstBI BstEII
BstPI BstUI ClaI CpoI DraIII
EagI EclXI Eco52I EcoO65I EcoRV
EcoT22I FseI FspI Hin1I HpaI
KasI KpnI KspI MfeI MluI
MunI MvnI NarI NcoI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 83 TCCGGA T^CCGGA
AflII 1369 CTTAAG C^TTAAG
AflIII 3577 ACACGT A^C[GA][CT]GT
AhdI 1938 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1938 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3694 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2405 TGGCCA TGG^CCA
BamHI 899 GGATCC G^GATCC
BanI 2613 GGTGCC G^G[CT][GA]CC
BbrPI 628 CACGTG CAC^GTG
BbsI 3057 GTCTTC GAAGAC(2/6)
BcgI 3178 CGAACAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1369 CTTAAG C^TTAAG
BglII 1039 AGATCT A^GATCT
BpuAI 3057 GTCTTC GAAGAC(2/6)
BsaBI 4010 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 83 TCCGGA [AT]^CCGG[AT]
BseAI 83 TCCGGA T^CCGGA
Bsp1407I 762 TGTACA T^GTACA
BspEI 83 TCCGGA T^CCGGA
BsrFI 3024 GCCGGC [GA]^CCGG[CT]
BsrGI 762 TGTACA T^GTACA
BssSI 2173 CACGAG CACGAG(-5/-1)
Bst1107I 4465 GTATAC GTA^TAC
BstAPI 196 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 770 CCAGGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4465 GTATAC GTA^TAC
Cfr10I 3024 GCCGGC [GA]^CCGG[CT]
DraI 3594 TTTAAA TTT^AAA
DrdI 2051 GACACAGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 94 CCACGG C^C[GA][CT]GG
Eam1105I 1938 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2620 GAATTC G^AATTC
MamI 4010 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2405 TGGCCA TGG^CCA
MroI 83 TCCGGA T^CCGGA
MscI 2405 TGGCCA TGG^CCA
NaeI 3024 GCCGGC GCC^GGC
NdeI 1493 CATATG CA^TATG
NgoMIV 3024 GCCGGC G^CCGGC
NheI 2070 GCTAGC G^CTAGC
PaeR7I 1866 CTCGAG C^TCGAG
PflFI 3694 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 628 CACGTG CAC^GTG
PmlI 628 CACGTG CAC^GTG
SacI 3610 GAGCTC GAGCT^C
SphI 3081 GCATGC GCATG^C
SspBI 762 TGTACA T^GTACA
Tth111I 3694 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1866 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 3622 GTCTAC GT^[AC][GT]AC
4465 GTATAC
Aor51HI 1889 AGCGCT AGC^GCT
4133 AGCGCT
AvaI 1687 CCCGAG C^[CT]CG[GA]G
1866 CTCGAG
BciVI 2291 GGATAC GTATCC(6/5)
1683 GTATCC
BclI 1034 TGATCA T^GATCA
3738 TGATCA
BglI 1138 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2407 GCCAAGTGGGC
BlpI 1812 GCTAAGC GC^T[ACGT]AGC
4156 GCTGAGC
Bpu1102I 1812 GCTAAGC GC^T[ACGT]AGC
4156 GCTGAGC
BsaAI 628 CACGTG [CT]AC^GT[GA]
3099 CACGTA
BsaI 1046 GAGACC GGTCTC(1/5)
1863 GGTCTC
BsoBI 1687 CCCGAG C^[CT]CG[GA]G
1866 CTCGAG
BsrBI 497 GAGCGG CCGCTC(-3/-3)
1856 GAGCGG
CelII 1812 GCTAAGC GC^T[ACGT]AGC
4156 GCTGAGC
CfoI 1890 GCGC GCG^C
4134 GCGC
Eco47III 1889 AGCGCT AGC^GCT
4133 AGCGCT
FauI 62 CCCGC CCCGC(4/6)
499 GCGGG
FbaI 1034 TGATCA T^GATCA
3738 TGATCA
HaeII 1889 AGCGCT [GA]GCGC^[CT]
4133 AGCGCT
HhaI 1890 GCGC GCG^C
4134 GCGC
HinP1I 1890 GCGC G^CGC
4134 GCGC
HincII 325 GTTGAC GT[CT]^[GA]AC
2005 GTCAAC
HindII 325 GTTGAC GT[CT]^[GA]AC
2005 GTCAAC
HindIII 748 AAGCTT A^AGCTT
1217 AAGCTT
PflMI 4052 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4087 CCATATCCTGG
Van91I 4052 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4087 CCATATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI Asp700I AspHI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI