Restriction map for
pF1KSDA1349
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI BsoBI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT14I FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI KspI MaeII MfeI
MflI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspI StyI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 1833 ACATGT A^C[GA][CT]GT
Alw44I 1675 GTGCAC G^TGCAC
ApaLI 1675 GTGCAC G^TGCAC
AseI 1234 ATTAAT AT^TAAT
AsnI 1234 ATTAAT AT^TAAT
AspHI 1675 GTGCAC G[AT]GC[AT]^C
AvaII 1601 GGACC G^G[AT]CC
BanII 4 GAGCCC G[GA]GC[CT]^C
BbvI 380 GCAGC GCAGC(8/12)
BsaBI 233 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1675 GTGCAC G[AT]GC[AT]^C
BspLU11I 1833 ACATGT A^CATGT
BspMI 12 ACCTGC ACCTGC(4/8)
BssKI 259 CCAGG ^CC[ACGT]GG
BstF5I 2105 GGATG GGATG(2/0)
BstNI 259 CCAGG CC^[AT]GG
Cac8I 563 GCCAGC GC[ACGT]^[ACGT]GC
DpnII 485 GATC ^GATC
EcoNI 1901 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 259 CCAGG ^CC[AT]GG
FauI 800 GCGGG CCCGC(4/6)
Fnu4HI 380 GCAGC GC^[ACGT]GC
FokI 2105 GGATG GGATG(9/13)
HindIII 806 AAGCTT A^AGCTT
ItaI 380 GCAGC GC^[ACGT]GC
MamI 233 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 485 GATC ^GATC
MvaI 259 CCAGG CC^[AT]GG
NdeII 485 GATC ^GATC
PpuMI 1600 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 1234 ATTAAT AT^TAAT
SapI 274 GAAGAGC GCTCTTC(1/4)
Sau3AI 485 GATC ^GATC
ScrFI 259 CCAGG CC^[ACGT]GG
SfcI 1062 CTATAG C^T[GA][CT]AG
SpeI 214 ACTAGT A^CTAGT
TaqI 2167 TCGA T^CGA
TseI 380 GCAGC G^C[AT]GC
Tsp45I 1837 GTCAC ^GT[GC]AC
TthHB8I 2167 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 1152 CCGC CCGC(-3/-1)
800 GCGG
BbsI 204 GAAGAC GAAGAC(2/6)
927 GAAGAC
BfaI 76 CTAG C^TAG
215 CTAG
BpuAI 204 GAAGAC GAAGAC(2/6)
927 GAAGAC
Cfr13I 1601 GGACC G^G[ACGT]CC
2039 GGCCC
DraI 324 TTTAAA TTT^AAA
1792 TTTAAA
DraII 1600 AGGACCC [GA]G^G[ACGT]CC[CT]
2038 AGGCCCT
EcoO109I 1600 AGGACCC [GA]G^G[ACGT]CC[CT]
2038 AGGCCCT
EcoT22I 1267 ATGCAT ATGCA^T
2107 ATGCAT
HphI 856 GGTGA GGTGA(8/7)
1484 TCACC
MaeI 76 CTAG C^TAG
215 CTAG
MwoI 640 GCCTTCATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1668 GCTTACCGTGC
NlaIV 3 GGAGCC GG[ACGT]^[ACGT]CC
1601 GGACCC
NsiI 1267 ATGCAT ATGCA^T
2107 ATGCAT
NspI 460 ACATGC [GA]CATG^[CT]
1833 ACATGT
Sau96I 1601 GGACC G^G[ACGT]CC
2039 GGCCC
SwaI 323 ATTTAAAT ATTT^AAAT
1791 ATTTAAAT
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BmyI BpmI BsiYI BslI BsmI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
DdeI EarI EcoRI HaeIII HinfI
Ksp632I MaeIII MboII MnlI MseI
MslI NlaIII PleI RsaI SfaNI
SmlI SspBI StuI TfiI Tru9I
Tsp509I TspRI XmnI