Restriction map for
pF1KSDA1355
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AclI AcyI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI AspI AviII AvrII BbeI
BbrPI BcgI BfrI BglII BlnI
BsaBI BsaHI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII EarI EcoO65I
EcoRV EcoT22I FspI Hin1I HincII
HindII HindIII HpaI KasI Ksp632I
MamI MfeI MluI MseI MunI
NarI NcoI NdeI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI Tru9I Tth111I
XbaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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Acc65I 1204 GGTACC G^GTACC
AccIII 1806 TCCGGA T^CCGGA
Aor51HI 3088 AGCGCT AGC^GCT
Asp718I 1204 GGTACC G^GTACC
AspHI 955 GTGCTC G[AT]GC[AT]^C
BclI 1139 TGATCA T^GATCA
BlpI 3343 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3343 GCTGAGC GC^T[ACGT]AGC
BsaI 289 GGTCTC GGTCTC(1/5)
BseAI 1806 TCCGGA T^CCGGA
BsiHKAI 955 GTGCTC G[AT]GC[AT]^C
BsmBI 2136 CGTCTC CGTCTC(1/5)
BspEI 1806 TCCGGA T^CCGGA
BssSI 2086 CTCGTG CACGAG(-5/-1)
CelII 3343 GCTGAGC GC^T[ACGT]AGC
DrdI 2349 GACCGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3088 AGCGCT AGC^GCT
FbaI 1139 TGATCA T^GATCA
KpnI 1204 GGTACC GGTAC^C
KspI 1879 CCGCGG CCGC^GG
MroI 1806 TCCGGA T^CCGGA
NheI 1752 GCTAGC G^CTAGC
PaeR7I 3050 CTCGAG C^TCGAG
SacII 1879 CCGCGG CCGC^GG
SfiI 1476 GGCCCGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1010 GCATGC GCATG^C
SspI 1280 AATATT AAT^ATT
XhoI 3050 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 3271 GAATTC [GA]^AATT[CT]
3442 GAATTC
AocI 70 CCTGAGG CC^T[ACGT]AGG
3412 CCTGAGG
ApoI 3271 GAATTC [GA]^AATT[CT]
3442 GAATTC
Asp700I 1810 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2935 GAATCACTTC
BciVI 2868 GGATAC GTATCC(6/5)
2923 GTATCC
BglI 1477 GCCCGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1979 GCCGGCAAGGC
BsaAI 241 TACGTG [CT]AC^GT[GA]
1504 TACGTG
BsaWI 1806 TCCGGA [AT]^CCGG[AT]
2146 TCCGGT
BsmI 1453 GAATGC GAATGC(1/-1)
1655 GAATGC
BspMI 125 ACCTGC ACCTGC(4/8)
613 ACCTGC
BsrBI 1264 GAGCGG CCGCTC(-3/-3)
3016 GAGCGG
BsrDI 911 GCAATG GCAATG(2/0)
1466 GCAATG
BstAPI 754 GCATACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2365 GCACCCTCTGC
BstXI 3069 CCAGAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3399 CCATGTTACTGG
Bsu36I 70 CCTGAGG CC^T[ACGT]AGG
3412 CCTGAGG
EagI 137 CGGCCG C^GGCCG
141 CGGCCG
EclXI 137 CGGCCG C^GGCCG
141 CGGCCG
Eco52I 137 CGGCCG C^GGCCG
141 CGGCCG
Eco81I 70 CCTGAGG CC^T[ACGT]AGG
3412 CCTGAGG
EcoRI 3271 GAATTC G^AATTC
3442 GAATTC
FseI 138 GGCCGGCC GGCCGG^CC
142 GGCCGGCC
StuI 2052 AGGCCT AGG^CCT
3030 AGGCCT
XmnI 1810 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2935 GAATCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AfaI AhdI
AluI AlwI AlwNI ApaI AspEI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiEI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI