Restriction map for
pF1KSDA1360
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI Asp700I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI Ksp632I
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 738 GGTACC G^GTACC
AccI 1082 GTCTAC GT^[AC][GT]AC
AflIII 489 ACATGT A^C[GA][CT]GT
Alw44I 1711 GTGCAC G^TGCAC
AlwNI 1675 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 472 CCTGAGG CC^T[ACGT]AGG
ApaLI 1711 GTGCAC G^TGCAC
AseI 804 ATTAAT AT^TAAT
AsnI 804 ATTAAT AT^TAAT
Asp718I 738 GGTACC G^GTACC
BanI 738 GGTACC G^G[CT][GA]CC
BanII 991 GAGCTC G[GA]GC[CT]^C
BbsI 260 GTCTTC GAAGAC(2/6)
BbvCI 772 GCTGAGG CCTCAGC(-5/-2)
BglII 2370 AGATCT A^GATCT
BpuAI 260 GTCTTC GAAGAC(2/6)
BsaBI 532 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 778 GAATGC GAATGC(1/-1)
BspLU11I 489 ACATGT A^CATGT
BspMI 1212 ACCTGC ACCTGC(4/8)
BsrDI 2357 GCAATG GCAATG(2/0)
BstXI 2176 CCAAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 472 CCTGAGG CC^T[ACGT]AGG
DraI 2364 TTTAAA TTT^AAA
DraII 236 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 1292 TGGCCG [CT]^GGCC[GA]
Eco81I 472 CCTGAGG CC^T[ACGT]AGG
EcoNI 2203 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 236 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 1174 GATATC GAT^ATC
HapII 1295 CCGG C^CGG
HincII 388 GTCAAC GT[CT]^[GA]AC
HindII 388 GTCAAC GT[CT]^[GA]AC
HpaII 1295 CCGG C^CGG
HphI 1277 TCACC GGTGA(8/7)
KpnI 738 GGTACC GGTAC^C
MaeII 2255 ACGT A^CGT
MamI 532 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1288 CAGCTG C[AC]G^C[GT]G
MspI 1295 CCGG C^CGG
PpuMI 236 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 804 ATTAAT AT^TAAT
PvuII 1288 CAGCTG CAG^CTG
SacI 991 GAGCTC GAGCT^C
ScaI 1825 AGTACT AGT^ACT
SfcI 1977 CTACAG C^T[GA][CT]AG
SmlI 2207 CTCAAG C^T[CT][GA]AG
Tsp45I 2002 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AciI 1102 GCGG CCGC(-3/-1)
1216 GCGG
AvaII 237 GGACC G^G[AT]CC
1218 GGTCC
BpmI 5 CTGGAG CTGGAG(16/14)
1423 CTGGAG
BseRI 2204 CTCCTC GAGGAG(10/8)
1492 GAGGAG
BsgI 575 GTGCAG GTGCAG(16/14)
688 GTGCAG
BsmFI 236 GGGAC GGGAC(10/14)
2242 GGGAC
DsaI 947 CCATGG C^C[GA][CT]GG
1847 CCATGG
NcoI 947 CCATGG C^CATGG
1847 CCATGG
NheI 856 GCTAGC G^CTAGC
2353 GCTAGC
NspI 489 ACATGT [GA]CATG^[CT]
1949 ACATGC
SfaNI 532 GATGC GCATC(5/9)
1621 GCATC
XcmI 1280 CCAAAGAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2006 CCAAGATGACTCTGG
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
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AcsI AfaI AluI AlwI ApoI
AspHI BbvI BfaI BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SspI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII