Restriction map for
pF1KSDA1368
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccIII AflII AflIII AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BfrI BglII BlnI BseAI BsiEI
BsmBI BspDI BspEI BspLU11I Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FseI FspI HindIII
HpaI KasI KpnI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PmaCI PmeI PmlI PshAI PshBI
PvuI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 819 GACGTC GACGT^C
AcyI 819 GACGTC G[GA]^CG[CT]C
AgeI 2743 ACCGGT A^CCGGT
Alw44I 2962 GTGCAC G^TGCAC
ApaLI 2962 GTGCAC G^TGCAC
Asp700I 1075 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 1618 GGTGCC G^G[CT][GA]CC
BglI 2566 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2163 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2163 GCTCAGC GC^T[ACGT]AGC
BsaAI 2536 TACGTG [CT]AC^GT[GA]
BsaHI 819 GACGTC G[GA]^CG[CT]C
BsaI 1586 GAGACC GGTCTC(1/5)
BsiWI 3055 CGTACG C^GTACG
Bsp1407I 3136 TGTACA T^GTACA
BspHI 188 TCATGA T^CATGA
BspMI 2456 ACCTGC ACCTGC(4/8)
BsrBI 1186 CCGCTC CCGCTC(-3/-3)
BsrGI 3136 TGTACA T^GTACA
BssHII 835 GCGCGC G^CGCGC
BssSI 2826 CACGAG CACGAG(-5/-1)
BstYI 790 GGATCT [GA]^GATC[CT]
CelII 2163 GCTCAGC GC^T[ACGT]AGC
CpoI 622 CGGACCG CG^G[AT]CCG
DraII 2715 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1303 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 2778 CTCTTC CTCTTC(1/4)
EcoO109I 2715 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 472 GAATTC G^AATTC
EcoT22I 389 ATGCAT ATGCA^T
HaeII 2574 GGCGCT [GA]GCGC^[CT]
Hin1I 819 GACGTC G[GA]^CG[CT]C
Ksp632I 2778 CTCTTC CTCTTC(1/4)
KspI 2364 CCGCGG CCGC^GG
MflI 790 GGATCT [GA]^GATC[CT]
NdeI 497 CATATG CA^TATG
NsiI 389 ATGCAT ATGCA^T
PaeR7I 3000 CTCGAG C^TCGAG
PflMI 1591 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2743 ACCGGT A^CCGGT
PpuMI 2715 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 188 TCATGA T^CATGA
RsrII 622 CGGACCG CG^G[AT]CCG
SacI 2119 GAGCTC GAGCT^C
SacII 2364 CCGCGG CCGC^GG
SmaI 2721 CCCGGG CCC^GGG
SphI 1466 GCATGC GCATG^C
SspBI 3136 TGTACA T^GTACA
StuI 2705 AGGCCT AGG^CCT
Van91I 1591 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 295 TCTAGA T^CTAGA
XhoI 3000 CTCGAG C^TCGAG
XhoII 790 GGATCT [GA]^GATC[CT]
XmaCI 2721 CCCGGG C^CCGGG
XmaI 2721 CCCGGG C^CCGGG
XmnI 1075 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 310 GTAGAC GT^[AC][GT]AC
2059 GTCTAC
AclI 517 AACGTT AA^CGTT
939 AACGTT
AcsI 263 AAATTT [GA]^AATT[CT]
472 GAATTC
ApoI 263 AAATTT [GA]^AATT[CT]
472 GAATTC
AspHI 2119 GAGCTC G[AT]GC[AT]^C
2962 GTGCAC
AspI 580 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3036 GACAAGGTC
BciVI 447 GGATAC GTATCC(6/5)
111 GTATCC
BclI 185 TGATCA T^GATCA
2073 TGATCA
BsaBI 180 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
906 GATTCGTATC
BsgI 2409 CTGCAC GTGCAG(16/14)
2101 GTGCAG
BsiHKAI 2119 GAGCTC G[AT]GC[AT]^C
2962 GTGCAC
BsmI 1856 GAATGC GAATGC(1/-1)
1782 GCATTC
BsrFI 1538 GCCGGT [GA]^CCGG[CT]
2743 ACCGGT
BstAPI 523 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
976 GCAGTCTGTGC
Cfr10I 1538 GCCGGT [GA]^CCGG[CT]
2743 ACCGGT
FbaI 185 TGATCA T^GATCA
2073 TGATCA
HincII 2328 GTCAAC GT[CT]^[GA]AC
2791 GTTGAC
HindII 2328 GTCAAC GT[CT]^[GA]AC
2791 GTTGAC
MamI 180 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
906 GATTCGTATC
MseI 362 TTAA T^TAA
2279 TTAA
PflFI 580 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3036 GACAAGGTC
Psp1406I 517 AACGTT AA^CGTT
939 AACGTT
SmlI 1829 CTCAAG C^T[CT][GA]AG
3000 CTCGAG
Tru9I 362 TTAA T^TAA
2279 TTAA
Tth111I 580 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3036 GACAAGGTC
XcmI 1262 CCAAAATTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1591 CCATATTGTGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I