Restriction map for
pF1KSDA1369
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BanI
BbeI BbvCI BcgI BfrI BlnI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
BspDI BspEI BspHI BsrBI BssHII
BstAPI BstBI BstEII BstPI Bsu36I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
HindIII KasI KpnI KspI MamI
MluI MroI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AflIII 705 ACATGT A^C[GA][CT]GT
Alw44I 1315 GTGCAC G^TGCAC
ApaI 96 GGGCCC GGGCC^C
ApaLI 1315 GTGCAC G^TGCAC
Asp700I 703 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1244 TGGCCA TGG^CCA
BamHI 411 GGATCC G^GATCC
BbrPI 232 CACGTG CAC^GTG
BclI 1359 TGATCA T^GATCA
BglI 1029 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1323 AGATCT A^GATCT
BlpI 844 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 844 GCTGAGC GC^T[ACGT]AGC
BsaAI 232 CACGTG [CT]AC^GT[GA]
BsaI 1710 GAGACC GGTCTC(1/5)
Bsp1407I 1538 TGTACA T^GTACA
BspLU11I 705 ACATGT A^CATGT
BspMI 655 GCAGGT ACCTGC(4/8)
BsrDI 795 GCAATG GCAATG(2/0)
BsrFI 72 GCCGGC [GA]^CCGG[CT]
BsrGI 1538 TGTACA T^GTACA
BssSI 725 CACGAG CACGAG(-5/-1)
Bst1107I 541 GTATAC GTA^TAC
BstZ17I 541 GTATAC GTA^TAC
CelII 844 GCTGAGC GC^T[ACGT]AGC
Cfr10I 72 GCCGGC [GA]^CCGG[CT]
DraIII 690 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 594 CCTGATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 464 CCTTGG C^C[AT][AT]GG
FbaI 1359 TGATCA T^GATCA
HpaI 1765 GTTAAC GTT^AAC
MfeI 1010 CAATTG C^AATTG
MluNI 1244 TGGCCA TGG^CCA
MscI 1244 TGGCCA TGG^CCA
MunI 1010 CAATTG C^AATTG
NaeI 72 GCCGGC GCC^GGC
NgoMIV 72 GCCGGC G^CCGGC
NspI 705 ACATGT [GA]CATG^[CT]
PmaCI 232 CACGTG CAC^GTG
PmlI 232 CACGTG CAC^GTG
PpuMI 629 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1381 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1732 CTGCAG CTGCA^G
SalI 960 GTCGAC G^TCGAC
SspBI 1538 TGTACA T^GTACA
StyI 464 CCTTGG C^C[AT][AT]GG
Tsp45I 329 GTGAC ^GT[GC]AC
XcmI 1004 CCAGTTCAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 703 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 541 GTATAC GT^[AC][GT]AC
960 GTCGAC
AccII 31 CGCG CG^CG
693 CGCG
AlwNI 1021 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1726 CAGCTGCTG
BbsI 312 GTCTTC GAAGAC(2/6)
659 GTCTTC
BciVI 529 GGATAC GTATCC(6/5)
434 GTATCC
BfaI 1051 CTAG C^TAG
1561 CTAG
BpuAI 312 GTCTTC GAAGAC(2/6)
659 GTCTTC
BseRI 37 GAGGAG GAGGAG(10/8)
1054 GAGGAG
BsgI 1138 CTGCAC GTGCAG(16/14)
1395 CTGCAC
BstUI 31 CGCG CG^CG
693 CGCG
BstXI 975 CCAGGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1749 CCAAAATTCTGG
CfoI 30 GCGC GCG^C
67 GCGC
DraII 95 AGGGCCC [GA]G^G[ACGT]CC[CT]
629 AGGTCCT
EaeI 74 CGGCCA [CT]^GGCC[GA]
1244 TGGCCA
EcoO109I 95 AGGGCCC [GA]G^G[ACGT]CC[CT]
629 AGGTCCT
FauI 88 CCCGC CCCGC(4/6)
1200 GCGGG
HhaI 30 GCGC GCG^C
67 GCGC
HinP1I 30 GCGC G^CGC
67 GCGC
MaeI 1051 CTAG C^TAG
1561 CTAG
MaeIII 329 GTGAC ^GT[ACGT]AC
485 GTTAC
MvnI 31 CGCG CG^CG
693 CGCG
PleI 958 GAGTC GAGTC(4/5)
1222 GAGTC
SspI 673 AATATT AAT^ATT
1339 AATATT
TspRI 888 CACTG CA[GC]TG[ACGT][ACGT]^
1661 CAGTG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp509I
TthHB8I XhoII